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Nitrate reduction rates and sediment physicochemical conditions from a natural and constructed marsh in coastal Alabama from 2018-11-30 to 2019-09-06 (NCEI Accession 0210961)

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This dataset includes nitrate reduction rates and physicochemical characteristics from sediments collected from a natural (NAT) and a 32-year-old constructed (CON-2) marsh on the Alabama (USA) coast. Three cores were collected from each marsh on three dates (30 November 2018, 26 March 2019, 06 September 2019) to a depth of 25 cm. Cores were partitioned to 5 cm depth increments for all measurements. Nitrogen removal (denitrification) and retention (dissimilatory nitrate reduction to ammonium, DNRA) were measured with and without glucose additions using isotope pairing on sediment slurries. Plant belowground biomass, extractable ammonium, and organic matter content were measured as well. The percent contribution of denitrification to total nitrate reduction was calculated to determine how much nitrogen is removed vs. retained.

Dataset Citation

Cite as: Tatariw, Corianne; Mortazavi, Behzad; Ledford, Taylor C.; Starr, Sommer F.; Smyth, Erin; Griffin Wood, Abigail; Simpson, Loraé T.; Cherry, Julia (2020). Nitrate reduction rates and sediment physicochemical conditions from a natural and constructed marsh in coastal Alabama from 2018-11-30 to 2019-09-06 (NCEI Accession 0210961). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0210961. Accessed [date].

Dataset Identifiers

ISO 19115-2 Metadata

gov.noaa.nodc:0210961

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Distribution Formats	XLSX CSV PDF
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Distributor	NOAA National Centers for Environmental Information +1-301-713-3277 ncei.info@noaa.gov
Dataset Point of Contact	NOAA National Centers for Environmental Information ncei.info@noaa.gov

Time Period	2018-11-30 to 2019-09-06
Spatial Bounding Box Coordinates	West: -88.163156 East: -88.149499 South: 30.365625 North: 30.369162
Spatial Coverage Map

General Documentation

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Descriptive Information
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Publication Dates	publication: 2020-07-10
Data Presentation Form	Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status	Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility
Data Update Frequency	As needed
Purpose	This dataset is available to the public for a wide variety of uses including scientific research and analysis.
Use Limitations	accessLevel: Public Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.

Dataset Citation	Cite as: Tatariw, Corianne; Mortazavi, Behzad; Ledford, Taylor C.; Starr, Sommer F.; Smyth, Erin; Griffin Wood, Abigail; Simpson, Loraé T.; Cherry, Julia (2020). Nitrate reduction rates and sediment physicochemical conditions from a natural and constructed marsh in coastal Alabama from 2018-11-30 to 2019-09-06 (NCEI Accession 0210961). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0210961. Accessed [date].
Cited Authors	Tatariw, Corianne University of Alabama Mortazavi, Behzad Ledford, Taylor C. Smithsonian Environmental Research Center Starr, Sommer F. Smyth, Erin Griffin Wood, Abigail Simpson, Loraé T. Cherry, Julia
Contributors	University of Alabama (UA)
Resource Providers	Corianne Tatariw University of Alabama (UA) University of Alabama (UA)
Points of Contact	Corianne Tatariw University of Alabama (UA)
Publishers	NOAA National Centers for Environmental Information
Acknowledgments	Funding Agency: National Science Foundation CBET 1643486 Funding Agency: National Science Foundation CBET 1438092

Theme keywords	NODC DATA TYPES THESAURUS CARBON - ORGANIC - SEDIMENT CHEMISTRY - SEDIMENT SEDIMENT PROPERTIES NODC OBSERVATION TYPES THESAURUS in situ laboratory analyses WMO_CategoryCode oceanography Global Change Master Directory (GCMD) Science Keywords EARTH SCIENCE > OCEANS > MARINE SEDIMENTS EARTH SCIENCE > OCEANS > MARINE SEDIMENTS > SEDIMENT CHEMISTRY EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > ORGANIC CARBON Provider Keywords Constructed marsh DNRA Rates (+C) DNRA Rates (Ambient) Denitrification Rate (Ambient) Denitrification Rates (+C) Depth Interval Marsh Natural marsh Percent denitrification (+C) Percent denitrification (Ambient) Plant Belowground Biomass Sediment extractable ammonium Sediment organic matter content
Data Center keywords	NODC COLLECTING INSTITUTION NAMES THESAURUS University of Alabama NODC SUBMITTING INSTITUTION NAMES THESAURUS University of Alabama
Instrument keywords	NODC INSTRUMENT TYPES THESAURUS fluorometer mass spectrometer Global Change Master Directory (GCMD) Instrument Keywords FLUOROMETERS > FLUOROMETERS MASS SPECTROMETERS > MASS SPECTROMETERS Provider Instruments Balance Calendar Membrane inlet mass spectrometer Muffle furnace, balance Ruler
Place keywords	NODC SEA AREA NAMES THESAURUS Coastal Waters of Alabama Gulf of Mexico Global Change Master Directory (GCMD) Location Keywords OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN > GULF OF MEXICO Provider Place Names Mobile Bay, Alabama
Keywords	NCEI ACCESSION NUMBER 0210961
Keywords	Send2NCEI Submission Package ID DMBF58

Use Constraints	Cite as: Tatariw, Corianne; Mortazavi, Behzad; Ledford, Taylor C.; Starr, Sommer F.; Smyth, Erin; Griffin Wood, Abigail; Simpson, Loraé T.; Cherry, Julia (2020). Nitrate reduction rates and sediment physicochemical conditions from a natural and constructed marsh in coastal Alabama from 2018-11-30 to 2019-09-06 (NCEI Accession 0210961). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0210961. Accessed [date].
Access Constraints	Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
Fees	In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.

Lineage information for: dataset
Processing Steps	2020-07-10T18:46:16Z - NCEI Accession 0210961 v1.1 was published.
Output Datasets	NCEI Accession 0210961 v1.1 NCEI Accession 0210961 v1.1 (download) published 2020-07-10T18:46:16Z

Lineage information for: dataset
Processing Steps	Parameter or Variable: Plant Belowground Biomass (measured); Units: kg/m^2; Observation Category: laboratory analysis; Sampling Instrument: balance; Sampling and Analyzing Method: Cores were collected from three locations per marsh to 25 cm depth using a metal t-corer (i.d.=7.9 cm). Cores were extruded onto a tray and subdivided into 5 cm increments by depth (i.e. 0-5 cm, 5-10 cm, 10-15 cm, 15-20 cm, 20-25 cm). Back at the lab, roots were separated from soil by rinsing with deionized water through a 2 mm sieve. The remaining plant material was oven dried at 60°C to a constant weight.. Parameter or Variable: Sediment extractable ammonium (measured); Units: nmol N/g; Observation Category: laboratory analysis; Sampling Instrument: fluorometer; Sampling and Analyzing Method: Cores were collected from three locations per marsh to 25 cm depth using a metal t-corer (i.d.=7.9 cm). Cores were extruded onto a tray and subdivided into 5 cm increments by depth (i.e. 0-5 cm, 5-10 cm, 10-15 cm, 15-20 cm, 20-25 cm). Each 5 cm section was sub-sampled using a syringe corer (i.d.=1.3 cm). Soil extractable NH4+ was determined by mixing soil with 2 N potassium chloride (KCl) on a shaker table. After 24 hours, slurries were centrifuged and the supernatant was filtered (0.45 µm nylon membrane filter) and frozen until analysis. Soil extractable NH4+ was determined fluorometrically Turner Designs 7200-002 fluorometer equipped with a CDOM/NH4 UV module.. Parameter or Variable: Sediment organic matter content (measured); Units: %; Observation Category: laboratory analysis; Sampling Instrument: muffle furnace, balance; Sampling and Analyzing Method: Cores were collected from three locations per marsh to 25 cm depth using a metal t-corer (i.d.=7.9 cm). Cores were extruded onto a tray and subdivided into 5 cm increments by depth (i.e. 0-5 cm, 5-10 cm, 10-15 cm, 15-20 cm, 20-25 cm). Each 5 cm section was sub-sampled using a syringe corer (i.d.=1.3 cm). Soil percent organic matter (%OM) was determined by loss-on-ignition (LOI). Samples were oven dried at 60°C and ground with a mortar and pestle. After re-drying in the oven, sub-samples were ashed in a muffle furnace (Lindberg/Blue M Moldatherm Box Furnace, Thermo Scientific) at 600°C for six hours. Sediment organic matter content was not measured on 2018-11-30.. Parameter or Variable: Denitrification Rate (Ambient) (measured); Units: µmol N kg dry weight-1 h-1; Observation Category: laboratory analysis; Sampling Instrument: Membrane inlet mass spectrometer; Sampling and Analyzing Method: Cores were collected from three locations per marsh to 25 cm depth using a metal t-corer (i.d.=7.9 cm). Cores were extruded onto a tray and subdivided into 5 cm increments by depth (i.e. 0-5 cm, 5-10 cm, 10-15 cm, 15-20 cm, 20-25 cm). Sediment samples were stored in Ziploc bags and homogenized manually. Roughly one quarter of each sample was slurried with artificial sea water (ASW) adjusted to the salinity of the West Fowl River at the time of sampling (~10 PPT). Slurries were bubbled with N2 gas to produce anoxic conditions and siphoned into 12 mL Exetainer vials (Labco). Following an overnight incubation on a shaker table to remove residual NO3- and O2, slurries were spiked to ~50 µM Na15NO3 (99 atom %; Cambridge Isotope Laboratories, Inc.). One slurry tube for each sample was immediately spiked with zinc chloride (ZnCl2, 50% W/V) to stop biological activity (t0). Three slurry tubes per sample were incubated for ~6 hours at ambient water temperature then spiked with ZnCl2 (t6). Denitrification was measured based on the concentration of 29N2 and 30N2 concentrations in slurry water with a membrane inlet mass spectrometer (MIMS) outfitted with a copper reduction column to remove O2.. Parameter or Variable: DNRA Rates (Ambient) (measured); Units: µmol N kg dry weight-1 h-1; Observation Category: laboratory analysis; Sampling Instrument: Membrane inlet mass spectrometer; Sampling and Analyzing Method: Cores were collected from three locations per marsh to 25 cm depth using a metal t-corer (i.d.=7.9 cm). Cores were extruded onto a tray and subdivided into 5 cm increments by depth (i.e. 0-5 cm, 5-10 cm, 10-15 cm, 15-20 cm, 20-25 cm). Sediment samples were stored in Ziploc bags and homogenized manually. Roughly one quarter of each sample was slurried with artificial sea water (ASW) adjusted to the salinity of the West Fowl River at the time of sampling (~10 PPT). Slurries were bubbled with N2 gas to produce anoxic conditions and siphoned into 12 mL Exetainer vials (Labco). Following an overnight incubation on a shaker table to remove residual NO3- and O2, slurries were spiked to ~50 µM Na15NO3 (99 atom %; Cambridge Isotope Laboratories, Inc.). One slurry tube for each sample was immediately spiked with zinc chloride (ZnCl2, 50% W/V) to stop biological activity (t0). Three slurry tubes per sample were incubated for ~6 hours at ambient water temperature then spiked with ZnCl2 (t6). DNRA tubes were bubbled with N2 to purge 29N2 and 30N2 produced by denitrification. Samples were then amended with 200 µL sodium hypobromite, which converts NH4+ to N2. The resulting 29N2 and 30N2 concentrations were measured on the MIMS.. Parameter or Variable: Denitrification Rates (+C) (measured); Units: µmol N kg dry weight-1 h-1; Observation Category: laboratory analysis; Sampling Instrument: Membrane inlet mass spectrometer; Sampling and Analyzing Method: Denitrification rates were measured as described for Denitrification Rates (Ambient), but one t6 tube for each sample was amended with glucose solution (final concentration: ~50 µM C) at the beginning of the incubation.. Parameter or Variable: DNRA Rates (+C) (measured); Units: µmol N kg dry weight-1 h-1; Observation Category: laboratory analysis; Sampling Instrument: Membrane inlet mass spectrometer; Sampling and Analyzing Method: DNRA rates were measured as described for DNRA Rates (Ambient), but one t6 tube for each sample was amended with glucose solution (final concentration: ~50 µM C) at the beginning of the incubation.. Parameter or Variable: Percent denitrification (Ambient) (calculated); Units: %; Observation Category: other; Sampling Instrument: other (calculation); Sampling and Analyzing Method: To determine the percent of nitrate removed by denitrification out of total NO3- reduction was calculated on ambient rates: [Denitrification/(Denitrification+DNRA)] x 100. Parameter or Variable: Percent denitrification (+C) (calculated); Units: %; Observation Category: other; Sampling Instrument: other (calculation); Sampling and Analyzing Method: To determine the percent of nitrate removed by denitrification out of total NO3- reduction was calculated on +C rates: [Denitrification/(Denitrification+DNRA)] x 100. Parameter or Variable: Collection Date (measured); Units: yyyy-mm-dd; Observation Category: other; Sampling Instrument: calendar; Sampling and Analyzing Method: Date that samples were collected in the field.. Parameter or Variable: Marsh (measured); Units: none; Observation Category: other; Sampling Instrument: none; Sampling and Analyzing Method: Refers to marsh type sampled. NAT = natural marsh, CON-2 = constructed marsh. Parameter or Variable: Replicate (measured); Units: none; Observation Category: other; Sampling Instrument: none; Sampling and Analyzing Method: Triplicate samples were collected from each marsh on each sampling date.. Parameter or Variable: Depth Interval (measured); Units: cm; Observation Category: in situ; Sampling Instrument: ruler; Sampling and Analyzing Method: Sediment cores (25 cm) were divided into 5-5cm depth intervals (0-5 cm, 5-10 cm, 10-15 cm, 15-20 cm, 20-25 cm).

Lineage information for: dataset

Processing Steps

Parameter or Variable: Plant Belowground Biomass (measured); Units: kg/m^2; Observation Category: laboratory analysis; Sampling Instrument: balance; Sampling and Analyzing Method: Cores were collected from three locations per marsh to 25 cm depth using a metal t-corer (i.d.=7.9 cm). Cores were extruded onto a tray and subdivided into 5 cm increments by depth (i.e. 0-5 cm, 5-10 cm, 10-15 cm, 15-20 cm, 20-25 cm). Back at the lab, roots were separated from soil by rinsing with deionized water through a 2 mm sieve. The remaining plant material was oven dried at 60°C to a constant weight..
Parameter or Variable: Sediment extractable ammonium (measured); Units: nmol N/g; Observation Category: laboratory analysis; Sampling Instrument: fluorometer; Sampling and Analyzing Method: Cores were collected from three locations per marsh to 25 cm depth using a metal t-corer (i.d.=7.9 cm). Cores were extruded onto a tray and subdivided into 5 cm increments by depth (i.e. 0-5 cm, 5-10 cm, 10-15 cm, 15-20 cm, 20-25 cm). Each 5 cm section was sub-sampled using a syringe corer (i.d.=1.3 cm). Soil extractable NH4+ was determined by mixing soil with 2 N potassium chloride (KCl) on a shaker table. After 24 hours, slurries were centrifuged and the supernatant was filtered (0.45 µm nylon membrane filter) and frozen until analysis. Soil extractable NH4+ was determined fluorometrically Turner Designs 7200-002 fluorometer equipped with a CDOM/NH4 UV module..
Parameter or Variable: Sediment organic matter content (measured); Units: %; Observation Category: laboratory analysis; Sampling Instrument: muffle furnace, balance; Sampling and Analyzing Method: Cores were collected from three locations per marsh to 25 cm depth using a metal t-corer (i.d.=7.9 cm). Cores were extruded onto a tray and subdivided into 5 cm increments by depth (i.e. 0-5 cm, 5-10 cm, 10-15 cm, 15-20 cm, 20-25 cm). Each 5 cm section was sub-sampled using a syringe corer (i.d.=1.3 cm). Soil percent organic matter (%OM) was determined by loss-on-ignition (LOI). Samples were oven dried at 60°C and ground with a mortar and pestle. After re-drying in the oven, sub-samples were ashed in a muffle furnace (Lindberg/Blue M Moldatherm Box Furnace, Thermo Scientific) at 600°C for six hours. Sediment organic matter content was not measured on 2018-11-30..
Parameter or Variable: Denitrification Rate (Ambient) (measured); Units: µmol N kg dry weight-1 h-1; Observation Category: laboratory analysis; Sampling Instrument: Membrane inlet mass spectrometer; Sampling and Analyzing Method: Cores were collected from three locations per marsh to 25 cm depth using a metal t-corer (i.d.=7.9 cm). Cores were extruded onto a tray and subdivided into 5 cm increments by depth (i.e. 0-5 cm, 5-10 cm, 10-15 cm, 15-20 cm, 20-25 cm). Sediment samples were stored in Ziploc bags and homogenized manually. Roughly one quarter of each sample was slurried with artificial sea water (ASW) adjusted to the salinity of the West Fowl River at the time of sampling (~10 PPT). Slurries were bubbled with N2 gas to produce anoxic conditions and siphoned into 12 mL Exetainer vials (Labco). Following an overnight incubation on a shaker table to remove residual NO3- and O2, slurries were spiked to ~50 µM Na15NO3 (99 atom %; Cambridge Isotope Laboratories, Inc.). One slurry tube for each sample was immediately spiked with zinc chloride (ZnCl2, 50% W/V) to stop biological activity (t0). Three slurry tubes per sample were incubated for ~6 hours at ambient water temperature then spiked with ZnCl2 (t6). Denitrification was measured based on the concentration of 29N2 and 30N2 concentrations in slurry water with a membrane inlet mass spectrometer (MIMS) outfitted with a copper reduction column to remove O2..
Parameter or Variable: DNRA Rates (Ambient) (measured); Units: µmol N kg dry weight-1 h-1; Observation Category: laboratory analysis; Sampling Instrument: Membrane inlet mass spectrometer; Sampling and Analyzing Method: Cores were collected from three locations per marsh to 25 cm depth using a metal t-corer (i.d.=7.9 cm). Cores were extruded onto a tray and subdivided into 5 cm increments by depth (i.e. 0-5 cm, 5-10 cm, 10-15 cm, 15-20 cm, 20-25 cm). Sediment samples were stored in Ziploc bags and homogenized manually. Roughly one quarter of each sample was slurried with artificial sea water (ASW) adjusted to the salinity of the West Fowl River at the time of sampling (~10 PPT). Slurries were bubbled with N2 gas to produce anoxic conditions and siphoned into 12 mL Exetainer vials (Labco). Following an overnight incubation on a shaker table to remove residual NO3- and O2, slurries were spiked to ~50 µM Na15NO3 (99 atom %; Cambridge Isotope Laboratories, Inc.). One slurry tube for each sample was immediately spiked with zinc chloride (ZnCl2, 50% W/V) to stop biological activity (t0). Three slurry tubes per sample were incubated for ~6 hours at ambient water temperature then spiked with ZnCl2 (t6). DNRA tubes were bubbled with N2 to purge 29N2 and 30N2 produced by denitrification. Samples were then amended with 200 µL sodium hypobromite, which converts NH4+ to N2. The resulting 29N2 and 30N2 concentrations were measured on the MIMS..
Parameter or Variable: Denitrification Rates (+C) (measured); Units: µmol N kg dry weight-1 h-1; Observation Category: laboratory analysis; Sampling Instrument: Membrane inlet mass spectrometer; Sampling and Analyzing Method: Denitrification rates were measured as described for Denitrification Rates (Ambient), but one t6 tube for each sample was amended with glucose solution (final concentration: ~50 µM C) at the beginning of the incubation..
Parameter or Variable: DNRA Rates (+C) (measured); Units: µmol N kg dry weight-1 h-1; Observation Category: laboratory analysis; Sampling Instrument: Membrane inlet mass spectrometer; Sampling and Analyzing Method: DNRA rates were measured as described for DNRA Rates (Ambient), but one t6 tube for each sample was amended with glucose solution (final concentration: ~50 µM C) at the beginning of the incubation..
Parameter or Variable: Percent denitrification (Ambient) (calculated); Units: %; Observation Category: other; Sampling Instrument: other (calculation); Sampling and Analyzing Method: To determine the percent of nitrate removed by denitrification out of total NO3- reduction was calculated on ambient rates: [Denitrification/(Denitrification+DNRA)] x 100.
Parameter or Variable: Percent denitrification (+C) (calculated); Units: %; Observation Category: other; Sampling Instrument: other (calculation); Sampling and Analyzing Method: To determine the percent of nitrate removed by denitrification out of total NO3- reduction was calculated on +C rates: [Denitrification/(Denitrification+DNRA)] x 100.
Parameter or Variable: Collection Date (measured); Units: yyyy-mm-dd; Observation Category: other; Sampling Instrument: calendar; Sampling and Analyzing Method: Date that samples were collected in the field..
Parameter or Variable: Marsh (measured); Units: none; Observation Category: other; Sampling Instrument: none; Sampling and Analyzing Method: Refers to marsh type sampled. NAT = natural marsh, CON-2 = constructed marsh.
Parameter or Variable: Replicate (measured); Units: none; Observation Category: other; Sampling Instrument: none; Sampling and Analyzing Method: Triplicate samples were collected from each marsh on each sampling date..
Parameter or Variable: Depth Interval (measured); Units: cm; Observation Category: in situ; Sampling Instrument: ruler; Sampling and Analyzing Method: Sediment cores (25 cm) were divided into 5-5cm depth intervals (0-5 cm, 5-10 cm, 10-15 cm, 15-20 cm, 20-25 cm).

Acquisition Information (collection)
Instrument	fluorometer mass spectrometer

Last Modified: 2024-09-17T19:17:08Z
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Nitrate reduction rates and sediment physicochemical conditions from a natural and constructed marsh in coastal Alabama from 2018-11-30 to 2019-09-06 (NCEI Accession 0210961)

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