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Laboratory carbon dioxide exposure experiments measuring scope for growth for juvenile Atlantic sea scallops (Placopecten magellanicus) from 2019-10-23 to 2019-12-19 (NCEI Accession 0276024)

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This dataset contains the biological response for Atlantic sea scallops (Placopecten magellanicus) exposed to three different levels of carbon dioxide enrichment (low, medium, high). The experiment took place from October 23, 2019 to December 19, 2019 (8 weeks). Salinity, temperature, dissolved oxygen, dissolved inorganic carbon, pH, chlorophyll-a, and seston counts are reported for the seawater during the 8 week exposure. Physiological measurements (feeding, respiration, and excretion rates) were taken 4 times during the experiment at the following temperatures (13.1C, 9.4C, 7.4C, and 6.1C). For feeding rates, the clearance rate, organic ingestion rate, assimilation rate, and assimilation efficiency are reported. From the respiration rate and excretion rate the atomic oxygen to nitrogen ratio is also reported. Scope for growth (the amount of energy available to grow) is calculated from the assimilated energy minus the energy for catabolic processes. Growth parameters were also taken during the 8 week experiment every 2 weeks. For growth parameters, dry tissue weight, dry shell weight, length, width, and thickness are reported.
  • Cite as: Pousse, Emilien; Poach, Matthew; Redman, Dylan H.; Sennefelder, George; Hubbard, William; Osborne, Kristin; Munroe, Daphne; Hart, Deborah R.; Hennen, Daniel R.; Dixon, Mark S.; Li, Yaqin; Milke, Lisa M.; Wikfors, Gary H.; Meseck, Shannon L. (2022). Laboratory carbon dioxide exposure experiments measuring scope for growth for juvenile Atlantic sea scallops (Placopecten magellanicus) from 2019-10-23 to 2019-12-19 (NCEI Accession 0276024). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/ftfq-zn70. Accessed [date].
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Distributor NOAA National Centers for Environmental Information
+1-301-713-3277
ncei.info@noaa.gov
Dataset Point of Contact NOAA National Centers for Environmental Information
ncei.info@noaa.gov
Time Period 2019-10-23 to 2019-12-19
Spatial Bounding Box Coordinates
West:
East:
South:
North:
Spatial Coverage Map
General Documentation
Associated Resources
  • NOAA National Centers for Environmental Information (2022). Ocean Carbon and Acidification Data System (OCADS). NOAA National Centers for Environmental Information. https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system
  • Enochs, Ian C.; Soderberg, Nash (2021). Lab-based measurements of carbonate substrate bioerosion and calcification using living date mussels (Lithophaga) collected outside Kaneohe Bay, at Site OAH-OCC-005, Hawaiian Islands, Pacific Ocean, from 2019-11-21 to 2020-01-08 (NCEI Accession 0224130). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/k43b-7176.
  • Feely, Richard A.; Alin, Simone R.; Hales, Burke; Johnson, Gregory C.; Juranek, Laurie W.; Byrne, Robert H.; Peterson, William T.; Goni, Miguel; Liu, Xuewu; Greeley, Dana (2015). Dissolved inorganic carbon, total alkalinity, pH, temperature, salinity and other variables collected from profile and discrete sample observations using CTD, Niskin bottle, and other instruments from R/V Wecoma in the U.S. West Coast California Current System during the 2011 West Coast Ocean Acidification Cruise (WCOA2011) from 2011-08-12 to 2011-08-30 (NCEI Accession 0123467). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.7289/v5jq0xz1.
  • Feely, Richard A.; Alin, Simone R.; Hales, Burke; Johnson, Gregory C.; Byrne, Robert H.; Peterson, William T.; Liu, Xuewu; Greeley, Dana (2015). Dissolved inorganic carbon, total alkalinity, pH on total scale and other variables collected from profile and discrete sample observations on NOAA Ship Fairweather (EXPOCODE 317W20130803) and R/V Point Sur (EXPOCODE 32P020130821) in the U.S. West Coast California Current System during the 2013 West Coast Ocean Acidification Cruise (WCOA2013) from 2013-08-03 to 2013-08-29 (NCEI Accession 0132082). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.7289/v5c53hxp.
  • Wieczorek, Daniel; Poach, Matthew (2014). Dissolved inorganic carbon, pH and other variables measured from laboratory experiment studies from an experimental carbonate exposure system from 2012-02-29 to 2012-05-11 (NCEI Accession 0123315). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.7289/v5z03646.
  • Wieczorek, Daniel; Poach, Matthew (2014). Dissolved inorganic carbon, pH and other variables measured from laboratory experiment studies from an experimental carbonate exposure system from 2013-05-15 to 2013-07-12 (NCEI Accession 0123316). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.7289/v5t72fdg.
  • Perry, Dean M.; Redman, Dylan H.; Widman, James C., Jr.; Meseck, Shannon L.; King, Andrew L.; Pereira, Jose J. (2014). Effects of ocean acidification on growth and otolith condition of juvenile scup, Stenotomus chrysops from laboratory experiment studies from 2011-08-24 to 2011-10-19 (NCEI Accession 0117506). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.7289/v5h70crk.
  • Slesinger, Emily; Mundorff, Samantha; Laurel, Benjamin J.; Hurst, Thomas P. (2024). Growth and development impacts of laboratory experimental ocean warming and ocean acidification conditions in Pacific cod embryos and larvae from 2022-04-06 to 2022-06-02 (NCEI Accession 0295839). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/bzqv-xz96.
  • Padilla, Dianna K.; Meseck, Shannon L. (2024). Blue mussel fertilization to early juvenile development under ocean acidification for 5 populations from Long Island Sound from 2017-04-20 to 2017-06-04 (NCEI Accession 0299732). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/614j-nz60.
  • Pousse, Emilien; Poach, Matthew; Redman, Dylan H.; Sennefelder, George; Munroe, Daphne; Hart, Deborah R.; Hennen, Daniel R.; Dixon, Mark S.; Li, Yaqin; Wikfors, Gary H.; Meseck, Shannon L. (2022). Biological effects of ocean acidification on juvenile Atlantic surfclams (Spisula solidissima) observed in laboratory experiments from 2019-04-10 to 2019-07-03 (NCEI Accession 0245952). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/fesj-kv54.
  • Gurr, Samuel J.; Meseck, Shannon L.; McFarland, Katherine (2025). Eastern oyster larva exposed to mutliple drivers of aragonite undersaturation from 2021-04-29 to 2021-05-21 (NCEI Accession 0300468). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/2ycy-7409.
  • Long, W. Christopher; Swiney, Katherine M.; Foy, Robert J. (2019). Effects of ocean acidification on the embryos and larvae of red king crab, Paralithodes camtschaticus (NCEI Accession 0170752). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/vkb2-nx25.
  • Meseck, Shannon L.; Alix, Jennifer H.; Swiney, Katherine M.; Long, W. Christopher; Wikfors, Gary H.; Foy, Robert J. (2015). The effects of ocean acidification on hemocyte of crab species in Alaska from laboratory experiment studies from 2011-07-01 to 2013-07-06 (NCEI Accession 0123400). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.7289/v59884zw.
  • Milke, Lisa M.; McCorkle, Daniel; Cohen, Anne; Widman, James C., Jr. (2014). The impact of ocean acidification on the early life stages of surf clams and the interactive effects of feeding and temperature from laboratory experiment studies from 2011-07-12 to 2012-06-17 (NCEI Accession 0123314). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.7289/v56h4fcz.
  • Hurst, Thomas P. (2015). Effects of ocean acidification on hatch size and larval growth of walleye pollock (Theragra chalcogramma) from laboratory experiment studies from 2010-03-01 to 2011-05-31 (NCEI Accession 0125007). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.7289/v5x0650t.
  • Swiney, Katherine M.; Long, W. Christopher; Foy, Robert J. (2019). The effects of ocean acidification and increased temperatures on the survival, growth, and morphology of red king crab (Paralithodes camtschaticus) (NCEI Accession 0170753). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/b9kq-wf98.
  • Stillman, Jonathon H.; Fay, Scott A.; Swiney, Katherine M.; Foy, Robert J. (2019). Transcriptomic Response of Juvenile Red King Crab, Paralithodes Camtschaticus, to the Interactive Effects of Ocean Acidification and Warming (NCEI Accession 0170754). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/gv7k-9315.
  • Long, W. Christopher; Swiney, Katherine M.; Foy, Robert J. (2022). Observations of embryo development and larval hatching of snow crabs (Chionoecetes opilio) collected in laboratory experiments for the Physiological Response of Commercially Important Crab Species to Predicted Increases in pCO2 Project in Kodiak, Alaska from 2014-08-06 to 2016-06-16 (NCEI Accession 0259411). NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0259411.
  • Long, W. Christopher; Swiney, Katherine M.; Foy, Robert J. (2022). Effects of ocean acidification on the survival and growth of young-of-the-year golden king crab (Lithodes aequispinus) observed in laboratory experiments for the Effects of Ocean Acidification on Federally Managed Crab Species in Alaska project from 2014-04-14 to 2014-08-19 (NCEI Accession 0243465). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/18ry-tq39.
  • Dickinson, Gary H.; Bejerano, Shai; Salvador, Trina; Makdisi, Christine; Patel, Shrey; Long, W. Christopher; Swiney, Katherine M.; Foy, Robert J.; Steffel, Brittan V.; Smith, Kathryn E.; Aronson, Richard B. (2022). Observations of the effects of ocean acidification on the exoskeleton in adult Tanner crabs (Chionoecetes bairdi) collected in laboratory experiments for the Effects of Ocean Acidification on Federally Managed Crab Species project in Kodiak, Alaska from 2011-06-21 to 2013-07-14 (NCEI Accession 0248337). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/sebb-sa64.
  • Meseck, Shannon L.; Redman, Dylan H.; Clark, Paul; Mercaldo-Allen, Renee; Perry, Dean M. (2021). Black sea bass, Centropristis striata, first 48 hour response to increased levels of carbon dioxide from 2013-07-13 to 2015-07-10 (NCEI Accession 0225335). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/swbw-0w83.
  • King, Andrew L.; Meseck, Shannon L.; Milke, Lisa M.; Wikfors, Gary H.; Jenkins, Bethany (2014). Effects of ocean acidification on phytoplankton physiology and nutrition for fishery-based food webs from laboratory experiment studies from 2011-05-31 to 2013-02-07 (NCEI Accession 0121255). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.7289/v51v5bx5.
  • Enochs, Ian C.; Morris, John T. (2021). Lab based measurements of ocean acidification on Caribbean bioeroders (endolithic algae and reef-excavating sponges): implications for coral reef persistence, Cheeca Rocks Reef, Florida Keys, Northwest Atlantic Ocean, from 2018-06-11 to 2018-07-12 (NCEI Accession 0224590). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/tjs5-hn36.
  • Bednaršek, Nina; Feely, Richard A. (2016). Limacina helicina shell dissolution due to ocean acidification in the California Current Ecosystem from 2011-08-11 to 2013-08-29 (NCEI Accession 0155173). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.7289/v5rn35z7.
  • Long, W. Christopher; Swiney, Katherine M.; Foy, Robert J. (2022). Observations of larval snow crab (Chionoecetes opilio) survival and conditions in laboratory experiments for the Physiological Response of Commercially Important Crab Species to Predicted Increases in pCO2 Project in Kodiak, Alaska from 2015-04-20 to 2016-06-13 (NCEI Accession 0259404). NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0259404.
  • Andrade, Jessica F.; Hurst, Thomas P.; Miller, Jessica A. (2018). The effect of ocean acidification and predation-associated cues on juvenile speckled sanddab from laboratory experiment studies from 2015-10-12 to 2016-01-22 (NCEI Accession 0175257). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/8k2g-v257.
  • Gobler, Christopher J.; Nye, Janet A.; Grear, Jason S.; Griffith, Andrew W.; Wallace, Ryan B.; Clark, Hannah R.; DePasquale, Elizabeth L. (2018). The impacts of ocean acidification and multiple estuarine stressors on early-life stage of bivalve shellfish from a laboratory experiment study (NCEI Accession 0172041). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.7289/v5z036gp.
  • Hurst, Thomas P.; Copeman, Louise A.; Andrade, Jessica F.; Stowell, Michelle A.; Al-Samarrie, Colleen E.; Sanders, Justin L.; Kent, Michael L. (2022). Laboratory experimental growth and development responses of larval walleye pollock (Gadus chalcogrammus) experiencing ocean acidification conditions from 2018-04-04 to 2018-05-17 (NCEI Accession 0258039). NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0258039.
  • Hurst, Thomas P.; Laurel, Benjamin J.; Hanneman, Eric H.; Haines, Scott A.; Ottmar, Michele L. (2017). The effect of ocean acidification and prey availability on the growth of larval northern rock sole (Lepidopsetta polyxystra) (NCEI Accession 0157081). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.7289/v5n877vh.
  • Long, W. Christopher; Pruisner, Paige; Swiney, Katherine M.; Foy, Robert J. (2022). Measurements of respiration, feeding, and growth of juvenile red and blue king crabs collected in laboratory experiments for the Effects of Ocean Acidification on Federally Managed Crab Species project in Kodiak, Alaska from 2013-06-10 to 2013-07-14 (NCEI Accession 0247209). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/z0tj-bg93.
  • Wieczorek, Daniel; Poach, Matthew (2014). Dissolved inorganic carbon, pH and other variables measured from laboratory experiment studies from an experimental carbonate exposure system from 2013-12-09 to 2014-01-31 (NCEI Accession 0123317). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.7289/v5pg1ppr.
  • Hurst, Thomas P. (2015). Effects of elevated CO2 levels on eggs and larvae of a North Pacific flatfish (northern rock sole, Lepidopsetta polyxystra) from laboratory experiment studies from 2012-02-01 to 2013-09-30 (NCEI Accession 0136906). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.7289/v5zw1hzw.
  • Hurst, Thomas P.; Copeman, Louise A.; Haines, Scott A.; Meredith, Summer D.; Daniels, Kamilya; Hubbard, Kalyn M. (2019). Effects of elevated carbon dioxide on Pacific cod larvae size and behavior from laboratory experiment studies from 2015-02-12 to 2016-07-02 (NCEI Accession 0202775). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/y8xm-3306.
  • Long, W. Christopher; Van Sant, Scott B.; Swiney, Katherine M.; Foy, Robert J. (2022). Observations of survival, growth, and morphology of blue king crabs collected in laboratory experiments for the Effects of Ocean Acidification on Federally Managed Crab Species project in Kodiak, Alaska from 2011-06-16 to 2012-06-18 (NCEI Accession 0247437). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/9734-ye13.
  • Meseck, Shannon L.; Gurr, Samuel J.; McFarland, Katherine; Padilla, Dianna K. (2025). Dissolved inorganic carbon, pH, alkalinity, temperature, salinity, and biological response variables (growth, survival, deformities) collected from laboratory experiments for the research project (OAP- Research Organization Registry 02bfn4816; grant # 21268) for bay scallop larvae, Argopecten irradians, for 2 generations conducted at the NOAA Milford Laboratory in Milford, Connecticut from 2021-07-26 to 2022-08-30 (NCEI Accession 0302112). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/92td-sm58.
  • Busch, D. Shallin; Maher, Michael; Thibodeau, Patricia S.; McElhany, Paul (2015). Effects of ocean acidification on shell condition and survival of Puget Sound pteropods from laboratory experiment studies from 2012-05-10 to 2012-07-12 (NCEI Accession 0125008). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.7289/v5s75d83.
  • Gurr, Samuel J.; McFarland, Katherine; Bernatchez, Genevieve; Dixon, Mark S.; Guy, Lisa; Milke, Lisa M.; Poach, Matthew; Hart, Deborah R.; Plough, Louis V.; Redman, Dylan H.; Sennefelder, George; Stiles, Sheila; Wikfors, Gary H.; Padilla, Dianna K.; Meseck, Shannon L. (2024). Effects of food supply on northern bay scallops (Argopecten irradians) reared under two pCO2 conditions from 2021-09-14 to 2021-10-26 (NCEI Accession 0289954). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/rnt3-6g61.
  • Long, W. Christopher; Swiney, Katherine M.; Foy, Robert J. (2024). Observations of larval red king crab (Paralithodes camtschatica) survival and conditions in laboratory experiments for the Physiological Response of Commercially Important Crab Species to Predicted Increases in pCO2 project in Kodiak, Alaska from 2017-04-10 to 2017-07-08 (NCEI Accession 0292530). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/9k0s-4905.
  • Hauzer, Hagar; Evans, David; Müller, Wolfgang; Rosenthal, Yair; Erez, Jonathan (2024). The effect of carbonate chemistry on trace elements incorporation in the laboratory cultured large benthic foraminifer Operculina ammonoides from 2018-06-03 to 2019-08-04 (NCEI Accession 0290763). NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0290763.
  • Schweitzer, Cara C.; Bonin, Carolina A.; Magel, Christopher; Hurst, Thomas P.; Horodysky, Andrij Z. (2024). Sensory and gene expression impacts of laboratory experimental ocean acidification conditions in juvenile Arctic cod (Boreogadus saida) from 2019-04-05 to 2019-07-16 (NCEI Accession 0292280). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/502b-2s30.
  • Punt, André E.; Poljak, Dušanka; Dalton, Michael G.; Foy, Robert J. (2015). Effects of ocean acidification on fishery yields and profits of red king crab in Bristol Bay from model studies (NCEI Accession 0127395). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.7289/v5ng4njc.
  • Meseck, Shannon L.; McFarland, Katherine; Gurr, Samuel J.; Wikfors, Gary H. (2025). Biological response variables of hemolymph (cell viability, mtROS, and mitochondrial membrane potential) and dissolved inorganic carbon, pH, alkalinity, temperature, salinity, and collected from laboratory experiments for the research project (OAP-Research Organization Registry 02bfn4816) grant # 21268) for adult bay scallop, Argopecten irradians from 2023-05-02 to 2023-05-16 (NCEI Accession 0306498). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/wq5f-6k66.
Publication Dates
  • publication: 2022-12-23
  • creation: 2022-12-13
Data Presentation Form Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status Complete - production of the data has been completed
Historical archive - data has been stored in an offline storage facility
Data Update Frequency As needed
Supplemental Information
This data package (Submission ID: BGGLEMYLP) was acquired by NCEI from the Scientific Data Integration System (SDIS) at the NOAA Pacific Marine Environmental Laboratory (PMEL) in accordance with the archival submission agreement between NCEI and PMEL.

The approximate collection location of the scallops in this laboratory study: Latitude: 43.593577, Longitude: -70.181889

The location of the laboratory where the experiment was conducted: Latitude: 41.737548, Longitude: -70.625153
Purpose This dataset is available to the public for a wide variety of uses including scientific research and analysis.
Use Limitations
  • accessLevel: Public
  • Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
Dataset Citation
  • Cite as: Pousse, Emilien; Poach, Matthew; Redman, Dylan H.; Sennefelder, George; Hubbard, William; Osborne, Kristin; Munroe, Daphne; Hart, Deborah R.; Hennen, Daniel R.; Dixon, Mark S.; Li, Yaqin; Milke, Lisa M.; Wikfors, Gary H.; Meseck, Shannon L. (2022). Laboratory carbon dioxide exposure experiments measuring scope for growth for juvenile Atlantic sea scallops (Placopecten magellanicus) from 2019-10-23 to 2019-12-19 (NCEI Accession 0276024). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/ftfq-zn70. Accessed [date].
Cited Authors
Principal Investigators
Collaborators
  • Mark S. Dixon
Contributors
Resource Providers
Publishers
Acknowledgments
  • Funding Information: NOAA Ocean Acidification Program (OA Growth Affects on Eastern oysters Atlantic surfclams and Atlantic sea scallops (Meseck NEFSC), 17911)
Theme keywords NODC DATA TYPES THESAURUS NODC OBSERVATION TYPES THESAURUS WMO_CategoryCode
  • oceanography
Global Change Master Directory (GCMD) Science Keywords OCADS Study Type
  • Laboratory experiment
Provider Keywords
  • Atlantic sea scallops
  • Placopecten magellanicus
Provider Variable Abbreviations
  • A
  • AE
  • AR
  • C
  • CR
  • Chla
  • DE
  • DIC
  • DO
  • DSW
  • DTW
  • Date
  • E
  • ER
  • Food
  • H
  • ID
  • MTpH
  • OIR
  • O_N
  • PIM
  • POM
  • R
  • RR
  • R_U
  • Rep
  • SFG
  • S_in
  • Salinity
  • T
  • TSM
  • Temp
  • W
  • pH
Data Center keywords NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS Global Change Master Directory (GCMD) Data Center Keywords
Instrument keywords NODC INSTRUMENT TYPES THESAURUS Global Change Master Directory (GCMD) Instrument Keywords
Place keywords NODC SEA AREA NAMES THESAURUS Global Change Master Directory (GCMD) Location Keywords
Project keywords NODC PROJECT NAMES THESAURUS Ocean Acidification Search Keywords
  • Ocean Acidification Program (OAP)
  • Ocean Carbon and Acidification Data System (OCADS) Project
Keywords NCEI ACCESSION NUMBER
Use Constraints
  • Cite as: Pousse, Emilien; Poach, Matthew; Redman, Dylan H.; Sennefelder, George; Hubbard, William; Osborne, Kristin; Munroe, Daphne; Hart, Deborah R.; Hennen, Daniel R.; Dixon, Mark S.; Li, Yaqin; Milke, Lisa M.; Wikfors, Gary H.; Meseck, Shannon L. (2022). Laboratory carbon dioxide exposure experiments measuring scope for growth for juvenile Atlantic sea scallops (Placopecten magellanicus) from 2019-10-23 to 2019-12-19 (NCEI Accession 0276024). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/ftfq-zn70. Accessed [date].
Access Constraints
  • Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
Fees
  • In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
Lineage information for: dataset
Processing Steps
  • 2022-12-23T19:11:22Z - NCEI Accession 0276024 v1.1 was published.
Output Datasets
Lineage information for: nonGeographicDataset
Processing Steps
  • Parameter or Variable: Dissolved Inorganic Carbon; Abbreviation: DIC; Unit: umol/kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: Measured; Sampling instrument: Polypropylene bottle; Analyzing instrument: Discrete dissolved inorganic carbon (DIC) measurement (Non-dispersive infrared, or NDIR); Detailed sampling and analyzing information: Certified reference material was used to ensure the accuracy of the measurements. The values were within 2.7 umol/kg of the reported CRM value; Standardization description: CRMs; Standardization frequency: daily; CRM manufacturer: Andrew Dickson's lab at Scripps Institute of Oceanography.; Preservation method: run within minutes of collection; Preservative volume: none; Preservative correction: none
  • Parameter or Variable: pH; Abbreviation: pH; pH scale: Seawater scale (SWS); Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: Measured; Sampling instrument: Polypropylene bottle; Analyzing instrument: Spectrophotometer; Detailed sampling and analyzing information: Temperature for pH analysis was ~20 to 21 C and recorded for each measurement. Tris was used to ensure accuracy with a standard deviation of 0.0014 for pH.; Standardization description: TRIS BUFFER, Andrew Dickson Acid; Standardization frequency: daily; Temperature of standardization: in situ; Temperature correction method: water bath; At what temperature was pH reported: 20 ◦C; Uncertainty: ± 0.0014
  • Parameter or Variable: Sample ID; Abbreviation: ID; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Duration: 8 weeks; Detailed sampling and analyzing information: Sampling id for carbon dioxide enrichment. Containers were enriched with either low, medium, or high CO2 using mass flow controllers.; Uncertainty: 0; Biological subject: Placopecten magellanicus; Species ID: 79718; Researcher name: Shannon Meseck; Researcher institution: NOAA NMFS
  • Parameter or Variable: Replication; Abbreviation: Rep; Unit: A, B, or C; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: Measured; Duration: 8 weeks; Detailed sampling and analyzing information: Replicate treatment for each carbon dioxide level. Each co2 enrichment level had 3 replicates (A, B, or C).; Biological subject: Placopecten magellanicus; Species ID: 79718
  • Parameter or Variable: Water temperature; Abbreviation: Temp; Unit: C; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: in-situ observation; Measured or calculated: Measured; Sampling instrument: YSI - handheld multi-parameter instrument; Analyzing instrument: YSI - handheld multi-parameter instrument; Duration: 8 weeks; Detailed sampling and analyzing information: Temperature of bin on each date; Biological subject: Placopecten magellanicus; Species ID: 79718
  • Parameter or Variable: Salinity; Abbreviation: Salinity; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: in-situ observation; Sampling instrument: YSI - handheld multi-parameter instrument; Duration: 8 weeks; Detailed sampling and analyzing information: Samples for salinity was taken on a YSI that uses conductivity. No units; Uncertainty: 0.1; Biological subject: Placopecten magellanicus; Species ID: Placopecten magellanicus; Researcher name: Shannon Meseck; Researcher institution: NOAA NMFS
  • Parameter or Variable: Temperature for pH analysis; Abbreviation: MTpH; Unit: C; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: in-situ observation; Measured or calculated: Measured; Sampling instrument: Thermometer; Analyzing instrument: Agilent Cary 100 Spectrometer; Duration: 8 weeks; Detailed sampling and analyzing information: Temperature that the pH was measured at. The CARY 100 is hooked up to a water bath. Temperature was measured during analysis and recorded to ensure accuracy of pH.; Biological subject: Placopecten magellanicus; Species ID: 79718
  • Parameter or Variable: Dissolved oxygen (DO); Abbreviation: DO; Unit: mg/l; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: in-situ observation; Measured or calculated: Measured; Sampling instrument: YSI - handheld multi-parameter instrument; Analyzing instrument: YSI - handheld multi-parameter instrument; Duration: 8 weeks; Detailed sampling and analyzing information: Dissolved oxygen in mg/L measured in each container; Uncertainty: 0.2; Biological subject: Placopecten magellanicus; Species ID: 79718; Researcher name: Shannon Meseck; Researcher institution: NOAA NMFS
  • Parameter or Variable: seston in buckets; Abbreviation: S_in; Unit: cells per ml; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: in-situ observation; Measured or calculated: Measured; Sampling instrument: Centrifuge tubes 50 ml; Analyzing instrument: Accuri C6+; Duration: 8 weeks; Detailed sampling and analyzing information: The incoming water was natural phytoplankton cells and the addition of Tetraselmis chui (PLY429) and Chaetoceros neogracile (Chaet-B). The counts were run on a Accuri C6+ (BD Biosceince San Jose, CA, USA) after being preserved in formaldehyde. For each sample 48 ml of seawater was taken with 2 ml of formaldehyde. The range was set on high chlorophyll-a region FL3 (633 nm) to count those cells. Samples were run for 30 seconds. During 30 seconds 33 ul of sample is analyzed. The count is what was being received by the sea scallops as food. Daily variability is because of variability in the natural seston and density of the two cultures of algae. On 11/24/2019 two samples were dropped during analysis breaking the tubes preventing cell counts for Medium A and Medium B that is why no cell count is reported.; Uncertainty: 5% of total counts; Biological subject: Placopecten magellanicus; Species ID: 79718; Researcher name: Shannon Meseck; Researcher institution: NOAA NMFS
  • Parameter or Variable: Date; Abbreviation: Date; Unit: d; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: in-situ observation; Measured or calculated: Measured; Sampling instrument: Calendar; Analyzing instrument: calendar; Duration: 8 weeks; Detailed sampling and analyzing information: Day that the sample was taken.; Uncertainty: 0; Biological subject: Placopecten magellanicus; Species ID: 79718; Researcher name: Shannon Meseck; Researcher institution: NOAA NMFS
  • Parameter or Variable: Height of shell; Abbreviation: H; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: digital vernier caliper; Analyzing instrument: digital vernier caliper; Duration: 8 weeks; Detailed sampling and analyzing information: Digital vernier caliper were used to measure from the umbo to the outer shell margin.; Uncertainty: 0.01; Biological subject: Placopecten magellanicus; Species ID: 79718; Researcher name: Shannon Meseck; Researcher institution: NOAA NMFS
  • Parameter or Variable: Width of shell; Abbreviation: W; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: digital vernier caliper; Analyzing instrument: digital vernier caliper; Duration: 8 weeks; Detailed sampling and analyzing information: Using digital vernier caliper the cross section of the sea scallop was measured parallel to the umbo.; Uncertainty: 0.01; Biological subject: Placopecten magellanicus; Species ID: 79718; Researcher name: Shannon Meseck; Researcher institution: NOAA NMFS
  • Parameter or Variable: Thickness of shell; Abbreviation: T; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: digital vernier caliper; Analyzing instrument: digital vernier caliper; Duration: 8 week; Detailed sampling and analyzing information: Digital vernier caliper was used to measure the thickenss of the shell at the center. The sea scallop was kept closed for this measurement.; Uncertainty: 0.01; Biological subject: Placopecten magellanicus; Species ID: 79718
  • Parameter or Variable: Dry Tissue Weight; Abbreviation: DTW; Unit: g; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: Balance; Analyzing instrument: Balance; Duration: 8 Weeks; Detailed sampling and analyzing information: DTW was determined by shucking the soft tissues and drying in an oven at 60°C to constant weight. This was for 5 days.; Biological subject: Placopecten magellanicus; Species ID: 79718; Researcher name: Shannon Meseck; Researcher institution: NOAA NMFS
  • Parameter or Variable: Dry Shell Weight; Abbreviation: DSW; Unit: g; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: Balance; Analyzing instrument: Balance; Duration: 8 weeks; Detailed sampling and analyzing information: For DSW (g), all fouling organisms and particulate material were removed with a freshwater rinse, then shells were dried to constant weight at 60°C. This took 5 days of drying.; Biological subject: Placopecten magellanicus; Species ID: 79718; Researcher name: Shannon Meseck; Researcher institution: NOAA NMFS
  • Parameter or Variable: Total suspended material; Abbreviation: TSM; Unit: g/ml; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: in-situ observation; Measured or calculated: Measured; Sampling instrument: Balance; Analyzing instrument: balance; Duration: 8 weeks; Detailed sampling and analyzing information: Water samples were taken for TSM for the biodeposition method. This is the mean TSM for the biodeposition timepoint. Samples were taken every 20 minutes for a minimum of 3 hours on pre-weighed, pre-combusted (450°C for 4 hours) GF/C filters which were rinsed with isotonic ammonium formate after filtration. Filters were then dried at 60°C until constant weight when they were weighed again. TSM was the difference in dry filter weight- original filter weight divided by total volume of water filtered.; Biological subject: Placopecten magellanicus; Species ID: 79718; Researcher name: Shannon Meseck; Researcher institution: NOAA NMFS
  • Parameter or Variable: Particulate inorganic material; Abbreviation: PIM; Unit: mg/l; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: in-situ observation; Measured or calculated: Measured; Sampling instrument: Balance and oven; Analyzing instrument: balance and oven; Duration: 8 weeks; Detailed sampling and analyzing information: PIM is the amount of particulate inorganic material in the biodeposition method. Samples were taken every 20 minutes for 3 hours during biodeposition. The mean is reported for this timeperiod. This is how much of the material was inorganic when the scallops were feeding. Samples were filtered on pre-weighed, pre-combusted (450°C for 4 hours) GF/C filters which were rinsed with isotonic ammonium formate after filtration. The inorganic fraction of each filter were calculated by drying the filters (60°C) until constant weight (~ 5 days) and then combusting at 450°C for 4 hours and weighing. PIM is then calculated as the material on the filter minus the initial weight of the filter divided by the volume of water filtered.; Biological subject: Placopecten magellanicus; Species ID: 79718; Researcher name: Shannon Meseck; Researcher institution: NOAA NMFS
  • Parameter or Variable: Particulate organic material; Abbreviation: POM; Unit: mg/l; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: in-situ observation; Measured or calculated: Measured; Sampling instrument: balance and oven; Duration: 8 weeks; Detailed sampling and analyzing information: POM is the amount of particulate organic material in the biodeposition method. Samples were taken every 20 minutes for 3 hours. This is how much of the material was organic when the scallops were feeding. Samples were filtered on pre-weighed, pre-combusted (450°C for 4 hours) GF/C filters which were rinsed with isotonic ammonium formate after filtration. The organic fraction of each filter were calculated by drying the filters (60°C) until constant weight (~ 5 days) and then combusting at 450°C for 4 hours and weighing. POM is then calculated as the material on the filter minus the dry filter weight divided by the volume of water filtered. The mean for biodeposition is reported.; Biological subject: Placopecten magellanicus; Species ID: 79718; Researcher name: Shannon Meseck; Researcher institution: NOAA NMFS
  • Parameter or Variable: Day of carbon dioxide exposure; Abbreviation: DE; Unit: Day; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: Measured; Sampling instrument: Calendar; Duration: 8 Weeks; Detailed sampling and analyzing information: This is the number of days that the sea scallops were exposed to carbon dioxide enrichment. The start of the experiment was 10/23/2019 day zero until December 19, 019. For day 0, 63 individualls were sampled for initial measurements. Every other sampling time point 12 individuals were sampled.; Uncertainty: 0; Biological subject: Placopecten magellanicus; Species ID: 79718
  • Parameter or Variable: Clearance Rate; Abbreviation: CR; Unit: L/h; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: Balance and oven; Analyzing instrument: Balance; Duration: 8 weeks; Detailed sampling and analyzing information: Clearance rate is the amount of water moved through the sea scallop. All measurements were standardized to 0.1 g of dry tissue weight using a b factor of 0.68. On 12/18 sampling data there are missing values because of the COVID-19 pandemic samples got compromised. For all other dates the missing values are because after 5 hours of analysis the animal was not produce enough material to accurate measure their feces and pseudofeces on the filter.; Method reference: Galimany et al. 2013, 2017, 2018, Pousse et al. 2020; Biological subject: Placopecten magellanicus; Species ID: 79718
  • Parameter or Variable: Organic ingestion rate; Abbreviation: OIR; Unit: mg/h; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: Balance; Analyzing instrument: balance and oven; Duration: 8 weeks; Detailed sampling and analyzing information: The organic ingestion rate is the amount organic matter ingested. The rate was normalized to 0.1 g of dry tissue weight with a b factor 0f 0.68. On 12/18 sampling data there are missing values because of the COVID-19 pandemic samples got compromised. For all other dates the missing values are because after 5 hours of analysis the animal was not produce enough material to accurate measure their feces and pseudofeces on the filter.; Method reference: Galimany et al. 2013, 2017, 2018, Pousse et al. 2020; Biological subject: Placopecten magellanicus; Species ID: 79718
  • Parameter or Variable: Assimilation rate; Abbreviation: AR; Unit: mg/h; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: Balance; Analyzing instrument: balance and oven; Duration: 8 weeks; Detailed sampling and analyzing information: The assimilation rate is the amount of food that they ingest. All rates were normalized to 0.1 g of dry tissue weight with a b factor of 0.68. On 12/18 sampling data there are missing values because of the COVID-19 pandemic samples got compromised. For all other dates the missing values are because after 5 hours of analysis the animal was not produce enough material to accurate measure their feces and pseudofeces on the filter.; Method reference: Galimany et al. 2013, 2017, 2018, Pousse et al. 2020; Biological subject: Placopecten magellanicus; Species ID: 79718
  • Parameter or Variable: Assimilation efficiency; Abbreviation: AE; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: balance and oven; Duration: 8 weeks; Detailed sampling and analyzing information: The assimilation efficiency that sea scallops had on acquiring food. On 12/18 sampling data there are missing values because of the COVID-19 pandemic samples got compromised. For all other dates the missing values are because after 5 hours of analysis the animal was not produce enough material to accurate measure their feces and pseudofeces on the filter.; Method reference: Galimany et al. 2013, 2017, 2018, Pousse et al. 2020; Biological subject: Placopecten magellanicus; Species ID: 79718
  • Parameter or Variable: Respiration rate; Abbreviation: RR; Unit: mg O2/ h; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: Loligo systems; Analyzing instrument: Loligo systems; Duration: 8 weeks; Detailed sampling and analyzing information: Scallops first were starved in 0.35-µm-filtered seawater at appropriate CO2 enrichment for 24 hours so that basal metabolic rates for respiration (RR) could be measured; then placed in individual, closed chambers (68 ml minus biovolume of animal) filled with 0.35-µm-filtered seawater treated at appropriate CO2 enrichment. An eight-channel Loligo respirometry system (Viborg, Denmark) was used to measure RR on 7 individuals with 1 chamber as the control (empty shell). RR was calculated from a linear regression applied to oxygen depletion with the background noise from the control chamber subtracted out. To ensure that scallops were not stressed from low oxygen concentrations in the chamber, recordings were stopped once oxygen saturation reached 80%. Missing values were because oxygen levels were not depleted enough to determine an accurate rate. The rate was similar to the control chamber. All rates were normalized to 0.1 g of dry tissue weight and raised to a b factor of 0.71.; Method reference: Pousse et al. 2020; Biological subject: Placopecten magellanicus; Species ID: 79718
  • Parameter or Variable: Excretion Rate; Abbreviation: ER; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: Agilent Cary 100 Spectrometer; Analyzing instrument: Agilent Cary 100 Spectrometer; Duration: 8 weeks; Detailed sampling and analyzing information: The same individuals used in biodeposition were used to calculate ER. Scallops first were starved in 0.35-µm-filtered seawater at appropriate CO2 enrichment for 24 hours so that basal metabolic rates for excretion (ER) could be measured.ER samples were taken by removing 5 mL from each chamber for ammonia-N determination after respiration was done. HACH TNT-830 test kits (equivalent EPA 350.1, EPA 351.1, and EPA 351.2) were used to determine ammonia-N with a modified protocol detailed in Pousse et al. (15). Briefly, absorbance was measured at 690 nm after 3.5 h to allow for color development. The method had a detection limit of 0.02 mg L-1. Missing values are because the excretion rate was below the method of detection. All rates were normalized to 0.1 g of dry tissue weight and raised to a b factor of 0.40.; Uncertainty: 0.01; Method reference: Pousse et al. 2020; Biological subject: Placopecten magellanicus; Species ID: 79718
  • Parameter or Variable: Energy of Food; Abbreviation: Food; Unit: J/mg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: in-situ observation; Measured or calculated: Calculated; Duration: 8 weeks; Detailed sampling and analyzing information: The energetic content of the food (J mg-1) obtained from Δfood= (0.632+0.086 (%C))×4.184, where %C was the POM. All blank values are because there was no AE measured for a scallop therefore the Food energy could not be calculated.; Method reference: Pousse et al. 2020; Biological subject: Placopecten magellanicus; Species ID: 97918
  • Parameter or Variable: Assimilated energy; Abbreviation: A; Unit: J/h; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Calculated; Duration: 8 weeks; Detailed sampling and analyzing information: The absorbed energy (A) is calculated as A=CR*AE*Food. Blank values are because their was no CR or AE to calculate A. For samples on December 18, 2019 samples were compromised because of the COVID-19 pandemic. The blanks for the rest of the data is because the organisms did no feed during the 5 hours the measurement was going on.; Method reference: Pousse et al. 2020; Biological subject: Placopecten magellanicus; Species ID: 79718
  • Parameter or Variable: Catabolic energy; Abbreviation: R_U; Unit: J/h; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Calculated; Duration: 8 weeks; Detailed sampling and analyzing information: RR was converted to oxygen-catabolized energy (R) using 14.0 J/mg O2, ER converted to U using 24.83 J/mg NH4-N. The two were added together to get catabolic energy.; Method reference: Pousse et al. 2020; Biological subject: Placopecten magellanicus; Species ID: 79718
  • Parameter or Variable: Scope For Growth; Abbreviation: SFG; Unit: J/h; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Calculated; Duration: 8 weeks; Detailed sampling and analyzing information: The scope for growth is calculated from the assimilated energy (A) minus the sum of the catabolic energy (R_U).; Method reference: Pousse et al.; Biological subject: Placopecten magellanicus; Species ID: 79718
  • Parameter or Variable: atomic ratio of oxygen to nitrogen; Abbreviation: O_N; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Calculated; Duration: 8 weeks; Detailed sampling and analyzing information: The atomic ratio of RR divided by ER.; Method reference: Pousse et al. 2020; Biological subject: Placopecten magellanicus; Species ID: 79718
  • Parameter or Variable: Chlorophyll-a; Abbreviation: Chla; Unit: ug/l; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: in-situ observation; Measured or calculated: Measured; Sampling instrument: YSI EXO2; Analyzing instrument: YSI EXO2; Duration: 8 weeks; Detailed sampling and analyzing information: Chlorphyll-a samples were taken on a YSI EXO2 with a chlorophyll sensor. Missing values are when the sensor was taken offline for calibration and maintenance.; Uncertainty: 0.01; Biological subject: Placopecten magellanicus; Species ID: 79718
  • Parameter or Variable: Time; Abbreviation: T; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: in-situ observation; Measured or calculated: Measured; Sampling instrument: YSI EXO2; Analyzing instrument: YSI EXO2; Duration: 8 weeks; Detailed sampling and analyzing information: The time that the sample was taken. Time is reported in military time.; Biological subject: Placopecten magellanicus; Species ID: 79718
  • Parameter or Variable: Respiration rate at 12 C; Abbreviation: R; Unit: mg O2/h; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: Loligo systems; Analyzing instrument: Loligo systems; Duration: 8 weeks; Detailed sampling and analyzing information: Scallops first were starved in 0.35-µm-filtered seawater at appropriate CO2 enrichment for 24 hours so that basal metabolic rates for respiration (R) could be measured; then placed in individual, closed chambers (68 ml minus biovolume of animal) filled with 0.35-µm-filtered seawater treated at appropriate CO2 enrichment. An eight-channel Loligo respirometry system (Viborg, Denmark) was used to measure R on 7 individuals with 1 chamber as the control (empty shell). Respiration rates are at 12C. The data is fitted to a power function with weight on the x axis and the R rate on the y-axis. The exponent of the power function is used in physiological measurements to scale physiological RR to body weight.; Method reference: Pousse et al.; Biological subject: Placopecten magellanicus; Species ID: 79718
  • Parameter or Variable: excretion rate at 12 C; Abbreviation: E; Unit: mg N/h; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Sampling instrument: Agilent Cary 100 Spectrometer; Analyzing instrument: Agilent Cary 100 Spectrometer; Duration: 8 weeks; Detailed sampling and analyzing information: Scallops first were starved in 0.35-µm-filtered seawater at appropriate CO2 enrichment for 24 hours so that basal metabolic rates for respiration (E) could be measured; then placed in individual, closed chambers (68 ml minus biovolume of animal) filled with 0.35-µm-filtered seawater treated at appropriate CO2 enrichment. ER samples were taken by removing 5 mL from each chamber for ammonia-N determination. HACH TNT-830 test kits (equivalent EPA 350.1, EPA 351.1, and EPA 351.2) were used to determine ammonia-N with a modified protocol detailed in Pousse et al. (15). Briefly, absorbance was measured at 690 nm after 3.5 h to allow for color development. The method had a detection limit of 0.02 mg/l. Excretion rates are at 12C. The data is fitted to a power function with weight on the x axis and the E rate on the y-axis. The exponent of the power function is used in physiological measurements to scale physiological ER to body weight.; Uncertainty: 0.01 mg/l; Biological subject: Placopecten magellanicus; Species ID: 79718
  • Parameter or Variable: Clearance rate at 12 C; Abbreviation: C; Unit: l/h; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: Measured; Duration: 8 weeks; Detailed sampling and analyzing information: Clearance rate of sea scallops. Rates are at 12 C.; Method reference: Pousse et al. 2020; Biological subject: Placopecten magellanicus; Species ID: 79718
Acquisition Information (collection)
Instrument
  • bottle
  • calipers
  • mass spectrometer
  • multi-parameter water quality sensor
  • scale
  • thermometer
Last Modified: 2025-12-12T07:45:41Z
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