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NCCOS Assessment: Digitized histological slides for a collaborative project on Stony Coral Tissue Loss Disease (SCTLD) resistance and susceptibility in Orbicella faveolata, collected in Broward County and Florida Keys reefs between May-June 2021 (NCEI Accession 0303739)

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This data is part of a multi-investigator, multi-disciplinary collaboration called the SCTLD resistance research consortium (RRC) investigating resistance and susceptibility among Orbicella faveolata populations off Broward and the Lower Florida Keys. These digitized histological slides include tissue biopsies from wild O. faveolata colonies from two regions (the Kristin Jacobs Coral Reef Ecosystem Conservation Area in Broward County, and Looe Key and Sand Key in the Florida Keys National Marine Sanctuary) and three resistance categories (“Low Resistance”, “Medium Resistance”, “High Resistance”) collected in late-May – mid-June, 2021. All slides were stained with hematoxylin and eosin and scanned on a digital slide scanner to a maximum magnification of 40x. All images are in SVS format. This dataset only includes the raw histological slide collection and associated metadata from biopsied coral colonies. Results from the analysis of the histological slides will be available from partner labs in separate associated datasets.
  • Cite as: Walker, Brian; Aine, Hawthorne; Peters, Esther; Renegar, D. Abigail; Holstein, Daniel; Woodley, Cheryl (2025). NCCOS Assessment: Digitized histological slides for a collaborative project on Stony Coral Tissue Loss Disease (SCTLD) resistance and susceptibility in Orbicella faveolata, collected in Broward County and Florida Keys reefs between May-June 2021 (NCEI Accession 0303739). https://www.ncei.noaa.gov/archive/accession/0303739. In Walker, Brian; Aine, Hawthorne; Peters, Esther; Renegar, D. Abigail; Holstein, Daniel; Woodley, Cheryl. NCCOS Assessment: Digitized histological slides for a collaborative project on Stony Coral Tissue Loss Disease (SCTLD) resistance and susceptibility in Orbicella faveolata, collected in Broward County and Florida Keys reefs between 2021-05-28 and 2022-03-19. [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/f254-yd56. Accessed [date].
gov.noaa.nodc:0303739
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Distributor NOAA National Centers for Environmental Information
+1-301-713-3277
ncei.info@noaa.gov
Dataset Point of Contact NOAA National Centers for Environmental Information
ncei.info@noaa.gov
Time Period 2021-05-28 to 2021-06-21
Spatial Bounding Box Coordinates
West: -81.5263
East: -79.739933
South: 24.015609
North: 26.09
Spatial Coverage Map
General Documentation
Associated Resources
  • NCCOS Assessment: Digitized histological slides for a collaborative project on Stony Coral Tissue Loss Disease (SCTLD) resistance and susceptibility in Orbicella faveolata, collected in Broward County and Florida Keys reefs between 2021-05-28 and 2022-03-19
  • FWC Digital Pathology database (eSlide manager): FWC Digital Pathology | FWC (https://myfwc.com/research/about/resources/eslide/)
  • Renegar, D. A., Noren, H. K. G., Turner, N. R., Figueiredo, J., & Walker, B. K. (2024). Southeast Florida large Orbicella faveolata are highly fecund without evident disease intervention effects. Frontiers in Marine Science, 11. https://doi.org/10.3389/fmars.2024.1369222
  • Klein, A. M., Sturm, A. B., Eckert, R. J., Walker, B. K., Neely, K. L., & Voss, J. D. (2024). Algal symbiont genera but not coral host genotypes correlate to stony coral tissue loss disease susceptibility among Orbicella faveolata colonies in South Florida. Frontiers in Marine Science, 11. https://doi.org/10.3389/fmars.2024.1287457
  • National Centers for Coastal Ocean Science, 2025: NCCOS Assessment: Use of Omics to Support Coral Interventions: Proteomics of gametes collected from Acropora cervicornis, Acropora palmata, Orbicella faveolata to Optimize Sexual Reproduction in corals from Key Largo, FL, from 2022-08-13 to 2022-08-17, 2023-08-04 to 2023-08-05, and 2023-09-05, https://www.fisheries.noaa.gov/inport/item/73852.
  • Janech, Michael; Duselis, Elizabeth; Neely, Benjamin; Woodley, Cheryl. Raw sequence reads and assemblies for metagenomes from various stony corals in the Florida Keys in 2019, either with or without visible signs of active stony coral tissue loss disease. Accession: PRJNA726962 ID: 726962. National Center for Biotechnology Information (NCBI).2021. https://www.ncbi.nlm.nih.gov/bioproject/PRJNA726962/
  • ProteomeXchange dataset. (n.d.). https://doi.org/10.6019/pxd026192
  • ProteomeXchange dataset. (n.d.). https://doi.org/10.6019/pxd038195
    • Parent ID (indicates this dataset is related to other data):
    • gov.noaa.nodc:NCCOS-SCTLD-Histology-Florida
Publication Dates
  • publication: 2025-09-12
Data Presentation Form Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status Complete - production of the data has been completed
Historical archive - data has been stored in an offline storage facility
Data Update Frequency As needed
Purpose Stony coral tissue loss disease (SCTLD) is a lethal disease affecting Florida’s coral reefs since 2014 whose etiology and etiological agent(s) are still unknown at the time of writing. The purpose of this project is to identify functional characteristics of the SCTLD phenotype at a cellular level to gain insights into the pathological mechanisms governing this disease. Understanding the functional changes associated with disease progression and resistance will provide a foundation for developing new treatments and diagnostics for intervention and management of SCTLD and other coral diseases. These digitized slides were collected as part of the histological analysis performed by the Resistance Research Consortium (RRC), a multi-institutional and multi-disciplinary collaboration of scientists working to understand the mechanisms of susceptibility and resistance to SCTLD.
Use Limitations
  • accessLevel: Public
  • Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
Dataset Citation
  • Cite as: Walker, Brian; Aine, Hawthorne; Peters, Esther; Renegar, D. Abigail; Holstein, Daniel; Woodley, Cheryl (2025). NCCOS Assessment: Digitized histological slides for a collaborative project on Stony Coral Tissue Loss Disease (SCTLD) resistance and susceptibility in Orbicella faveolata, collected in Broward County and Florida Keys reefs between May-June 2021 (NCEI Accession 0303739). https://www.ncei.noaa.gov/archive/accession/0303739. In Walker, Brian; Aine, Hawthorne; Peters, Esther; Renegar, D. Abigail; Holstein, Daniel; Woodley, Cheryl. NCCOS Assessment: Digitized histological slides for a collaborative project on Stony Coral Tissue Loss Disease (SCTLD) resistance and susceptibility in Orbicella faveolata, collected in Broward County and Florida Keys reefs between 2021-05-28 and 2022-03-19. [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/f254-yd56. Accessed [date].
Cited Authors
Principal Investigators
Collaborators
Contributors
Resource Providers
Points of Contact
Publishers
Acknowledgments
  • Funding Agency: US DOC; NOAA; NOS; National Centers for Coastal Ocean Science (NCCOS)
  • Funding Agency: NOAA Coral Reef Conservation Program, Use of ‘omics for coral restoration and disease intervention #31618
  • Funding Agency: Florida Department of Environmental Protection Award No. B96800
  • Funding Agency: Coral Reef Conservation Program (CRCP)
  • Funding Agency: NOAA National Centers for Coastal Ocean Science (NCCOS)
Theme keywords NODC DATA TYPES THESAURUS NODC OBSERVATION TYPES THESAURUS WMO_CategoryCode
  • oceanography
CoRIS Discovery Thesaurus
  • Visual Images > Corals
  • Visual Images > Histology
  • Visual Images > Invertebrates
CoRIS Theme Thesaurus
  • EARTH SCIENCE > Biosphere > Zoology > Corals
  • EARTH SCIENCE > Biosphere > Zoology > Corals > Coral Condition
  • EARTH SCIENCE > Biosphere > Zoology > Corals > Coral Diseases
  • EARTH SCIENCE > Biosphere > Zoology > Corals > Coral Mortality
  • EARTH SCIENCE > Oceans > Marine Biology > Coral
Global Change Master Directory (GCMD) Science Keywords NCCOS Research Areas
  • NCCOS Research Priority > Stressor Impacts and Mitigation
  • NCCOS Research Topic > Regional Ecosystem Science
NCCOS Research Data Types
  • NCCOS Research Data Type > Field Experiment
Provider Keywords
  • Coral histology
  • Coral pathology
  • Digitized histology slides of diseased coral tissues
  • Orbicella faveolata
  • Stony coral tissue loss disease (SCTLD)
Data Center keywords NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS Global Change Master Directory (GCMD) Data Center Keywords
Instrument keywords NODC INSTRUMENT TYPES THESAURUS Global Change Master Directory (GCMD) Instrument Keywords Provider Instruments
  • Digital Slide Scanner (40x) with EasyScanInfinity software
  • n/a
Place keywords NODC SEA AREA NAMES THESAURUS CoRIS Place Thesaurus
  • COUNTRY/TERRITORY > United States of America > Florida > Broward County > Broward County (26N080W0006)
  • COUNTRY/TERRITORY > United States of America > Florida > Broward County > Fort Lauderdale (26N080W0003)
  • COUNTRY/TERRITORY > United States of America > Florida > Florida Reef Tract (26N080W0007)
  • COUNTRY/TERRITORY > United States of America > Florida > Monroe County > Florida Keys (24N081W0007)
  • COUNTRY/TERRITORY > United States of America > Florida > Monroe County > Long Key (24N080W0017)
  • COUNTRY/TERRITORY > United States of America > Florida > Monroe County > Looe Key Reef (24N081W0002)
  • COUNTRY/TERRITORY > United States of America > Florida > Monroe County > Sand Key (24N081W0012)
  • OCEAN BASIN > Atlantic Ocean
  • OCEAN BASIN > Atlantic Ocean > North Atlantic Ocean > Florida Reef Tract (26N080W0007)
  • OCEAN BASIN > Atlantic Ocean > North Atlantic Ocean > Florida Reef Tract > Biscayne Bay > Sand Key (25N080W0032)
  • OCEAN BASIN > Atlantic Ocean > North Atlantic Ocean > Florida Reef Tract > Broward County (26N080W0006)
  • OCEAN BASIN > Atlantic Ocean > North Atlantic Ocean > Florida Reef Tract > Florida Keys (24N081W0007)
  • OCEAN BASIN > Atlantic Ocean > North Atlantic Ocean > Florida Reef Tract > Fort Lauderdale (26N080W0003)
  • OCEAN BASIN > Atlantic Ocean > North Atlantic Ocean > Florida Reef Tract > Lower Florida Keys > Looe Key Reef (24N081W0002)
  • OCEAN BASIN > Atlantic Ocean > North Atlantic Ocean > Florida Reef Tract > Upper Florida Keys > Long Key (24N080W0017)
Global Change Master Directory (GCMD) Location Keywords NCCOS Regions of Study
  • NCCOS Research Location > Region > Atlantic Ocean
  • NCCOS Research Location > U.S. States and Territories > Florida
Provider Place Names
  • Kristin Jacob’s Coral Reef Ecosystem Conservation Area
Project keywords NODC PROJECT NAMES THESAURUS CRCP Project Name
  • 31618
  • NOAA Coral Reef Conservation Program, Use of ‘omics for coral restoration and disease intervention
Keywords NCEI ACCESSION NUMBER
Keywords Send2NCEI Submission Package ID
  • 8DM68G
Use Constraints
  • Cite as: Walker, Brian; Aine, Hawthorne; Peters, Esther; Renegar, D. Abigail; Holstein, Daniel; Woodley, Cheryl (2025). NCCOS Assessment: Digitized histological slides for a collaborative project on Stony Coral Tissue Loss Disease (SCTLD) resistance and susceptibility in Orbicella faveolata, collected in Broward County and Florida Keys reefs between May-June 2021 (NCEI Accession 0303739). https://www.ncei.noaa.gov/archive/accession/0303739. In Walker, Brian; Aine, Hawthorne; Peters, Esther; Renegar, D. Abigail; Holstein, Daniel; Woodley, Cheryl. NCCOS Assessment: Digitized histological slides for a collaborative project on Stony Coral Tissue Loss Disease (SCTLD) resistance and susceptibility in Orbicella faveolata, collected in Broward County and Florida Keys reefs between 2021-05-28 and 2022-03-19. [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/f254-yd56. Accessed [date].
Data License
Access Constraints
  • Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
Fees
  • In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
Lineage information for: dataset
Processing Steps
  • 2025-09-12T19:03:59Z - NCEI Accession 0303739 v1.1 was published.
Output Datasets
Lineage information for: dataset
Processing Steps
  • Parameter or Variable: Stony coral tissue loss disease (SCTLD) (measured); Units: n/a; Observation Category: in situ; Sampling Instrument: n/a
  • Parameter or Variable: Coral pathology (measured); Units: n/a; Observation Category: laboratory analysis; Sampling Instrument: n/a
  • Parameter or Variable: Coral histology (measured); Units: n/a; Observation Category: laboratory analysis; Sampling Instrument: n/a
  • Parameter or Variable: Orbicella faveolata (measured); Units: n/a; Observation Category: in situ; Sampling Instrument: n/a
  • Parameter or Variable: Digitized histology slides of diseased coral tissues (measured); Units: n/a; Observation Category: other; Sampling Instrument: Digital Slide Scanner (40x) with EasyScanInfinity software; Sampling and Analyzing Method: Tissue biopsies were taken from targeted coral colonies in situ and fixed in zinc-formalin to preserve tissue integrity. Histological sections were prepared by the US Geological Survey (USGS) under the direction of Aine Hawthorne using standard processing techniques including dehydrating tissues with a graduated series of alcohols, infiltrating tissues with paraffin wax, embedding tissues in paraffin blocks, sectioning tissues and mounting onto glass slides for staining. Staining with hematoxylin and eosin was performed by IDEXX BioAnalytics (Columbia, MO). Stained slides were scanned on a digital slide scanner at a magnification of 40x using EasyScanInfinity software. No histological analysis is included in this dataset-any data resulting from the analysis of the histological slides will be available from partner labs in separate associated datasets.; Data Quality Method: NA (no histological analysis is included in this dataset-any data resulting from the analysis of the histological slides will be available from partner labs in separate associated datasets).
Acquisition Information (collection)
Instrument
  • camera
Last Modified: 2025-10-01T22:49:42Z
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