Ship (station) name: R/V "Professor Vodyanitsky" (IBSS, Sevastopol)
Expedition: cruise number 13, July, 1982.
Parameters: depth,  temperature  (T),  chlorophyll"a" (chl"a"),
            phosphate (PO4),  nitrite (NO2), nitrate (NO3),
            oxygen (O2), primary production (Pr.P), .
Instruments and methods:  Depth,  temperature,  salinity were measured
with a bathysonde "Istok".  
Samples for PO4,  NO2,  NO3 and O2 were collected
from the most sampled depths.  The concentrations of PO4,  NO3 and  O2
were determined by the methods of Grasshoff (1976). For measurement of
chlorophyll"a" 3-5 l water was filtered through Whatman GF/C glass fi-
ber  filters.  Determination of chlorophyll was carried out soon after
sampling on board. The analysis followed the spectrophotometric method
(UNESCO,  1966).  Primary  production was measured using simulation in
situ.  Water samples from each depth were incubated in 0,25 l polycar-
bonate bottles, inoculated with purified NaH14CO3 (20 mk Ci or 74*10^4
Bq) and placed into the appropriate incubator compartment with flowing
seawater.  Each  of  the 8 incubator compartments was irradiated using
neutral lights filters to reduce light to the level  corresponding  to
each depth.  The incubator was exposed on desk for 4-5 h the day time,
usually from between 7 to 12 a.m.  Incubation temperature was the same
as  the at of the sea serface.  The light intensity for each incubator
section was 100, 63, 34, 12, 5, 2, 1 and 0,5% of surface (Io). Samples
for  measurement  of  primary production were filtered through 0,4 mkm
membrane filters.  Filtration was initiated under vacuum (0,1  to  0,2
atm).  Filters  were placed in scintillation coctail and radioactivity
was measured using a 1215 RackBeta (LKB Wallac Co.).
Accuracy: The error of: depth -  +-1 dbar;
                        temperature -  +-0,01 grad C
                        salinity -  +-0,03 %o
                        P-PO4 -  +-0,05 mkM
                        N-NO2 -  +-15%
                        N-NO3 -  +-2%
                        O2  -  +-1 mkM
                        chlorophyll"a" - +-0,01 mg/m^3
                        primary production - +-15%
Processing (if applied):
Principal investigator(s): Z.Z.Finenko (Chief of Department of ecological
                       physiology of phytoplankton,IBSS, Sevastopol, Ukraine)
Digitization: single entry at the IBSS (Sevastopol, Ukraine)
Remarks: Date are obtained by qualified staff and are reliable.
Completed by:  Z.Z.Finenko (Chief of Department of ecological
              physiology of phytoplankton,IBSS, Sevastopol, Ukraine)
Date: 29.05.1997
Source of data: IBSS-Department of ecological physiology of
                  phytoplankton (IBSS, Sevastopol, Ukraine)
