Ship (station) name: R/V "Professor Vodyanitsky" (IBSS, Sevastopol)
Expedition: cruise number 21, October, 1986.
Parameters: depth,  temperature (T), chlorophyll"a" (chl"a"),
            phaeopigments (Ph), phosphate  (PO4),  nitrite
            (NO2), nitrate (NO3), Zooplankton Biomasse
Instruments and methods:  Depth,  temperature,  salinity were measured
with a bathysonde "Istok". 
Samples for PO4, NO2 and NO3 were collected from
the most sampled depths.  The concentrations of PO4, NO2 and NO3  were
determined  by  the  methods  of Grasshoff (1976).  For measurement of
chlorophyll"a" and phaeopigments 3-5 l water were filtered through 0,6
mkm  membrane  filters.  Determination of chlorophyll"a" and phaeopig-
ments were carried out soon after sampling  on  board.  Chlorophyll"a"
and  phaeopigments  concentrations  were  determined  fluorimetrically
(Yentsch and Menzel,1965;  Lorenzen,1967). The analysis of these para-
meters  were  carried by use laboratory fluorimeter constracted on the
base of spectrophotometer "Specol" fitted with a red sensitive  photo-
multiplication,  blue lamp, blue filter and red filter. Calibration of
fluorimeter was made with pure chlorophyll"a" in 90%  acetone with its
concentration first measured on a spectrophotometers.  Primary produc-
tion was measured using simulation in situ.  Water samples  from  each
depth were incubated in 0,25 l polycarbonate bottles,  inoculated with
purified NaH14CO3 (20 mk Ci or 74*10^4 Bq) and placed into the approp-
riate incubator compartment with flowing seawater. Each of the 8 incu-
bator compartments was irradiated using neutral lights filters to  re-
duce light to the level corresponding to each depth. The incubator was
exposed on desk for 4-5 h the day time,  usually from between 7 to  12
a.m. Incubation temperature was the same as the at of the sea serface.
The light intensity for each incubator section was 100, 63, 34, 12, 5,
2, 1 and 0,5% of surface (Io). Samples for measurement of primary pro-
duction were filtered through 0,4 mkm membrane filters. Filtration was
initiated under vacuum (0,1 to 0,2 atm).  Filters were placed in scin-
tillation coctail and radioactivity was measured using a 1215 RackBeta
(LKB Wallac Co.).
Accuracy: The error of: depth -  +-1 dbar;
                        temperature -  +-0,01 grad C
                        salinity -  +-0,03 %o
                        P-PO4 -  +-0,05 mkM
                        N-NO2 -  +-15%
                        N-NO3 -  +-2%
                        chlorophyll"a" - +-0,01 mg/m^3
                        phaeopigments -  +-10%
                        primary production - +-15%
Processing (if applied):
Principal investigator(s): Z.Z.Finenko (Chief of Department of 
ecological physiology of phytoplankton,IBSS, Sevastopol, Ukraine)
Digitization: single entry at the IBSS (Sevastopol, Ukraine)
Remarks: Date are obtained by qualified staff and are reliable.
Completed by:  Z.Z.Finenko (Chief of Department of ecological
              physiology of phytoplankton,IBSS, Sevastopol, Ukraine)
Date: 29.05.1997
Source of data: IBSS-Department of ecological physiology of
                  phytoplankton (IBSS, Sevastopol, Ukraine)
