Ship (station) name: R/V "Professor Vodyanitsky" (IBSS, Sevastopol)
Expedition: cruise number 24, June, 1987.
Parameters: depth,  temperature (T), chlorophyll"a" (chl"a"),
            phaeopigments (Ph),  primary production (Pr.P),
            Secchi disk depth, Zooplankton Biomass
Instruments and methods:  Depth,  temperature,  salinity were measured
with a bathysonde "Istok". 
For  measurement of chlorophyll"a" and phaeopigments 3-5 l water were
filtered through 0,6 mkm membrane filters.  Determination 
of chlorophyll"a" and phaeopigments were carried out soon
after sampling on board. Chlorophyll"a" and phaeopigments concentrati-
ons were determined fluorimetrically (Yentsch and Menzel,1965;  Loren-
zen,1967).  The analysis of these parameters were carried by use labo-
ratory  fluorimeter constracted on the base of spectrophotometer "Spe-
col" fitted with a red sensitive photomultiplication,  blue lamp, blue
filter  and red filter.  Calibration of fluorimeter was made with pure
chlorophyll"a" in 90% acetone with its concentration first measured on
a spectrophotometers. Primary production was measured using simulation
in situ.  Water samples from each depth were incubated in 0,25 l poly-
carbonate  bottles,  inoculated  with  purified  NaH14CO3 (20 mk Ci or
74*10^4 Bq) and placed into the appropriate incubator compartment with
flowing seawater.  Each of the 8 incubator compartments was irradiated
using neutral lights filters to reduce light to the  level  correspon-
ding  to  each depth.  The incubator was exposed on desk for 4-5 h the
day time, usually from between 7 to 12 a.m. Incubation temperature was
the  same  as the at of the sea serface.  The light intensity for each
incubator section was 100,  63,  34,  12,  5, 2, 1 and 0,5% of surface
(Io).  Samples  for  measurement  of  primary production were filtered
through 0,4 mkm membrane filters. Filtration was initiated under vacu-
um (0,1 to 0,2 atm).  Filters were placed in scintillation coctail and
radioactivity was measured using a 1215 RackBeta (LKB Wallac  Co.).  A
white  disk  approximately 20 cm in diameter which is lowered into the
water.  The depth at wich the disk disappears from view is defined  to
be the Secchi depth. This depth my be related to a broad waveband dif-
fuse attenuation coefficient: K=1.44/Trans.S (Kirk,1986)
 Accuracy: The error of: depth -  +-1 dbar;
                        temperature -  +-0,01 grad C
                        salinity -  +-0,03 %o
                        chlorophyll"a" - +-0,01 mg/m^3
                        phaeopigments -  +-10%
                        primary production - +-15%
Processing (if applied):
Principal investigator(s): Z.Z.Finenko (Chief of Department of ecological
                       physiology of phytoplankton,IBSS, Sevastopol, Ukraine)
Digitization: single entry at the IBSS (Sevastopol, Ukraine)
Remarks: Date are obtained by qualified staff and are reliable.
Completed by:  Z.Z.Finenko (Chief of Department of ecological
              physiology of phytoplankton,IBSS, Sevastopol, Ukraine)
Date: 29.05.1997
Source of data: IBSS-Department of ecological physiology of
                  phytoplankton (IBSS, Sevastopol, Ukraine)
