Ship (station) name: R/V "Professor Vodyanitsky" (IBSS, Sevastopol)
Expedition: cruise number 29, August, 1989.
Parameters: depth,  temperature (T), salinity, phosphate (PO4),
            nitrite (NO2),  nitrate (NO3), oxygen (O2), chlorophyll"a"
            (chl"a"),  phaeopigments (Ph),  primary production (Pr.P),
            Bioluminensence.
Instruments and methods:  Depth,  temperature,  salinity were measured
with  a  bathysonde "Istok".  
Samples for PO4,  NO2,  NO3 and O2 were collected
from  the most sampled depths.  The concentrations of PO4,  NO3 and O2
were determined by the methods of Grasshoff (1976). For measurement of
chlorophyll"a" and phaeopigments 3-5 l water were filtered through 0,6
mkm membrane filters.  Determination of chlorophyll"a"  and  phaeopig-
ments  were  carried out soon after sampling on board.  Chlorophyll"a"
and  phaeopigments  concentrations  were  determined  fluorimetrically
(Yentsch and Menzel,1965;  Lorenzen,1967). The analysis of these para-
meters were carried by use laboratory fluorimeter constracted  on  the
base  of spectrophotometer "Specol" fitted with a red sensitive photo-
multiplication,  blue lamp, blue filter and red filter. Calibration of
fluorimeter was made with pure chlorophyll"a" in 90%  acetone with its
concentration first measured on a spectrophotometers.  Primary produc-
tion  was  measured using simulation in situ.  Water samples from each
depth were incubated in 0,25 l polycarbonate bottles,  inoculated with
purified NaH14CO3 (20 mk Ci or 74*10^4 Bq) and placed into the approp-
riate incubator compartment with flowing seawater. Each of the 8 incu-
bator  compartments was irradiated using neutral lights filters to re-
duce light to the level corresponding to each depth. The incubator was
exposed  on desk for 4-5 h the day time,  usually from between 7 to 12
a.m. Incubation temperature was the same as the at of the sea serface.
The light intensity for each incubator section was 100, 63, 34, 12, 5,
2, 1 and 0,5% of surface (Io). Samples for measurement of primary pro-
duction were filtered through 0,4 mkm membrane filters. Filtration was
initiated under vacuum (0,1 to 0,2 atm).  Filters were placed in scin-
tillation coctail and radioactivity was measured using a 1215 RackBeta
(LKB Wallac Co.).  Photosynthetically active irradiation was  continu-
ously measured with a pyrnometer sensor at 400-700 nm coupled to a re-
cording integrator.
 Accuracy: The error of: depth -  +-1 dbar;
                        temperature -  +-0,01 grad C
                        salinity -  +-0,03 %o
                        P-PO4 -  +-0,05 mkM
                        N-NO2 -  +-15%
                        N-NO3 -  +-2%
                        O2  -  +-1 mkM
                        chlorophyll"a" - +-0,01 mg/m^3
                        phaeopigments -  +-10%
                        primary production - +-15%
Processing (if applied):
Principal investigator(s):  L.V.Ctelmach,  D.L.Gvozdeva (Department of
                       ecological  physiology  of  phytoplankton,IBSS,
                       Sevastopol, Ukraine)
Digitization: single entry at the IBSS (Sevastopol, Ukraine)
Remarks: Date are obtained by qualified staff and are reliable.
Completed by:  Z.Z.Finenko (Chief of Department of ecological
              physiology of phytoplankton,IBSS, Sevastopol, Ukraine)
Date: 29.05.1997
Source of data: IBSS-Department of ecological physiology of
                  phytoplankton (IBSS, Sevastopol, Ukraine)
