Ship (station) name: R/V "Professor Vodyanitsky" (IBSS, Sevastopol)
Expedition: cruise number 7, December, 1979.
Parameters: depth, temperature (T), chlorophyll"a" (chl"a"),
            phosphate (PO4), particulate organic carbon (POC),
            primary production (Pr.P)
Instruments and methods:  Depth,  temperature,  salinity were measured
with a  bathysonde "Istok". 
Samples for PO4 were collected from the most sampled depths.
  The  concentrations  were  determined  by  the methods of
Grasshoff (1976). Samples for particulate organic carbon were filtered
onto precombusted 25 mm Whatman GF/C filters. Filters were analysed by
combustion in a CHN-analyzer.  For measurement of chlorophyll"a" 3-5 l
water was filtered through Whatman GF/C glass fiber filters.  Determi-
nation of chlorophyll was carried out soon after  sampling  on  board.
The analysis  followed  the spectrophotometric method (UNESCO,  1966).
Primary production was measured using simulation in situ.  Water samp-
les from  each  depth  were incubated in 0,25 l polycarbonate bottles,
inoculated with purified NaH14CO3 (20 mk Ci or 74*10^4 Bq) and  placed
into the appropriate incubator compartment with flowing seawater. Each
of the 8 incubator compartments was irradiated  using  neutral  lights
filters to reduce light to the level corresponding to each depth.  The
incubator was exposed on desk for 4-5 h the  day  time,  usually  from
between 7 to 12 a.m.  Incubation temperature was the same as the at of
the sea serface.  The light intensity for each incubator  section  was
100, 63,  34, 12, 5, 2, 1 and 0,5% of surface (Io). Samples for measu-
rement of primary production were filtered through  0,4  mkm  membrane
filters. Filtration was initiated under vacuum (0,1 to 0,2 atm).  Fil-
ters were placed in scintillation coctail and radioactivity was measu-
red using a 1215 RackBeta (LKB Wallac Co.).  Photosynthetically active
irradiation was continuously measured  with  a  pyrnometer  sensor  at
400-700 nm coupled to a recording integrator.  Diffuse attenuation co-
efficient was measured with a submersible quantum sensor of the spect-
rum at 480 nm.  The sensor has a cosine collector, a colour filter and
a selenium element.

Accuracy: The error of: depth -  +-1 dbar;
                        temperature -  +-0,01 grad C
                        salinity -  +-0,03 %o
                        P-PO4 -  +-0,05 mkM
                        POC -  +-5%
                        chlorophyll"a" - +-0,01 mg/m^3
                        primary production - +-15%
                        Irradiation - +-5%
                        Attenuation coefficient - +-0,01 m^-1
Processing (if applied):
Principal investigator(s): Z.Z.Finenko (Chief of Department of ecological
                       physiology of phytoplankton,IBSS, Sevastopol, Ukraine)
Digitization: single entry at the IBSS (Sevastopol, Ukraine)
Remarks: Date are obtained by qualified staff and are reliable.
Completed by:  Z.Z.Finenko (Chief of Department of ecological
              physiology of phytoplankton,IBSS, Sevastopol, Ukraine)
Date: 29.05.1997
Source of data: IBSS-Department of ecological physiology of
                  phytoplankton (IBSS, Sevastopol, Ukraine)
