
<!-- saved from url=(0087)https://mermaid.ncddc.noaa.gov/org/disl/RKiene/Lab_work/Antarctica/Ross_Sea/previewTEXT -->
<html><head><meta http-equiv="Content-Type" content="text/html; charset=UTF-8"></head><body><pre style="word-wrap: break-word; white-space: pre-wrap;">Metadata:
  Identification_Information:
    Citation:
      Citation_Information:
        Originator: DISL: Kiene Lab
        Originator: SUNY Environmental Science and Forestry: Kieber Lab
        Publication_Date: Unpublished material
        Title: Collaborative Research: Impact of Solar Radiation and Nutrients on Biogeochemical Cycling of Dimethylsulfoniopropionate (DMSP) and Dimethylsulfide (DMS) in the Ross Sea, Antarctica
        Online_Linkage: http://www.esf.edu/antarctica/
    Description:
      Abstract: This dataset contains transect data from two research cruises to the Ross Sea, Antarctica, aboard the RV Nathaniel B. Palmer (NBP) in December 2004 to January 2005 (NBP04-09) and in October to November 2005 (NBP05-08). Water samples were collected from the ship's underway pumping system in the aft laboratory, which has an intake at 4 m depth, and from CTD casts with Niskin bottle sampling (up to 737 m). Samples were analyzed for concentration and biological turnover rate of sulfur compounds, particularly dimethylsulfide (DMS) and dimethylsulfoniopropionate (DMSP).
      Purpose: To improve our understanding of the biogeochemical dynamics of dimethylsulfide (DMS), dimethylsulfoniopropionate (DMSP), and dimethyl sulfoxide (DMSO) in the Ross Sea, Antarctica, and to evaluate the impact of ultraviolet radiation and dissolved nutrients on organosulfur cycling in these waters.
    Time_Period_of_Content:
      Time_Period_Information:
        Range_of_Dates/Times:
          Beginning_Date: 20041220
          Ending_Date: 20051130
      Currentness_Reference: ground condition
    Status:
      Progress: Complete
      Maintenance_and_Update_Frequency: None Planned
    Spatial_Domain:
      Bounding_Coordinates:
        West_Bounding_Coordinate: -179.49
        East_Bounding_Coordinate: -166.97
        North_Bounding_Coordinate: -49.01
        South_Bounding_Coordinate: -77.64
    Keywords:
      Theme:
        Theme_Keyword_Thesaurus: None
        Theme_Keyword: DMS
        Theme_Keyword: dimethylsulfide
        Theme_Keyword: DMSOd
        Theme_Keyword: dissolved dimethylsulfoxide
        Theme_Keyword: DMSPt
        Theme_Keyword: total dimethylsulfoniopropionate
        Theme_Keyword: DMSPd
        Theme_Keyword: dissolved dimethylsulfoniopropionate
        Theme_Keyword: DMSPp
        Theme_Keyword: particulate dimethylsulfoniopropionate
        Theme_Keyword: chl a
        Theme_Keyword: chlorophyll a
        Theme_Keyword: Phaeocystis antarctica
        Theme_Keyword: organic sulfur cycle
        Theme_Keyword: ocean chemistry
        Theme_Keyword: sulfur compounds
        Theme_Keyword: carbon dioxide
        Theme_Keyword: atmospheric chemistry
        Theme_Keyword: climate change
        Theme_Keyword: algal-derived trace gases
        Theme_Keyword: water quality
      Theme:
        Theme_Keyword_Thesaurus: ISO 19115 Topic Category
        Theme_Keyword: oceans
        Theme_Keyword: biota
        Theme_Keyword: climatologyMeteorologyAtmosphere
      Place:
        Place_Keyword_Thesaurus: None
        Place_Keyword: Antarctica
        Place_Keyword: Ross Sea
        Place_Keyword: New Zealand
        Place_Keyword: Southern Ocean
        Place_Keyword: Polar
        Place_Keyword: South Pole
        Place_Keyword: DISL
        Place_Keyword: Dauphin Island Sea Lab
    Access_Constraints: Permission to access these data must be given by Dr. Ron Kiene.
    Use_Constraints: Acknowledgment of the Dauphin Island Sea Lab: Kiene Lab, State University of New York Environmental Science and Forestry, Woods Hole Oceanographic Institution, the Office of Polar Programs, and the National Science Foundation is required in products developed from these data, and such acknowledgment as is standard for citation and legal practices for data source is expected by users of these data. Users should be aware that comparison with other data sets for the same area from other time periods may be inaccurate due to inconsistencies resulting from changes in mapping conventions, data collection, and computer processes over time. The distributor shall not be liable for improper or incorrect use of these data, based on the description of appropriate/inappropriate uses described in the metadata document. The Kiene Lab makes no warranty regarding these data, expressed or implied, nor does the fact of distribution constitute such a warranty. The Kiene Lab cannot assume liability for any damages caused by any errors or omissions in these data, nor as a result of the failure of these data to function on a particular system. These data are not legal documents and are not to be used as such.


    Point_of_Contact:
      Contact_Information:
        Contact_Person_Primary:
          Contact_Person: Ron Kiene
          Contact_Organization: DISL: Kiene Lab
        Contact_Position: Principal Investigator
        Contact_Address:
          Address_Type: mailing and physical
          Address: 101 Bienville Blvd.
          City: Dauphin Island
          State_or_Province: Al
          Postal_Code: 36528
          Country: USA
        Contact_Voice_Telephone: 251-861-2141 ext. 2269 or 7525
        Contact_Electronic_Mail_Address: rkiene@disl.org
        Hours_of_Service: 8-5:00 CST
        Contact_Instructions: Please email Ron Kiene for further information.
    Native_Data_Set_Environment: Microsoft Excel
  Data_Quality_Information:
    Attribute_Accuracy:
      Attribute_Accuracy_Report: Post cruise, we discovered that the acidification procedure used to measure DMSP may result in loss of DMSP in samples containing colonial Phaeocystis sp. Acidification causes a very rapid loss of DMSP and some conversion into DMS. This was not noticed on the cruise and no corrections could be made.  The amount of loss for these samples is not known. Because we found this only when colonial Phaeocystis was present and since during NBP-0409 (January 2005) the phytoplankton population was dominated by single celled Phaeocystis and diatoms, it is likely not as severe a problem as it was during NBP-0508 (November cruise).
    Logical_Consistency_Report: N/A
    Completeness_Report: Data were obtained during two research cruises to the Ross Sea aboard the RV Nathaniel B. Palmer in December 2004 to January 2005 (NBP04-09) and in October to November 2005 (NBP05-08).
    Lineage:
      Process_Step:
        Process_Description: Two types of data were collected during the research cruises. 

A) Along-track transect samples were collected from the ship's underway pumping system, with an intake at 4 m depth, and additional CTD casts with Niskin bottle sampling were conducted approximately once per day at a depth of 4 m during NBP05-08.

B) Vertical profile samples were collected by CTD cast with Niskin bottle sampling up to a depth of 737 m.
        Process_Date: 20051130
  Entity_and_Attribute_Information:
    Detailed_Description:
      Entity_Type:
        Entity_Type_Label: NBP Transect Data DMS-DMSP-DMSO-physical
        Entity_Type_Definition: Transect sample data collected from the ship's underway pumping system, a flow-through fluorometer (fluorescence data), or CTD Niskin to 4 m depth. The data are organized into two MS Excel spreadsheets, one for each cruise.
        Entity_Type_Definition_Source: DISL: Kiene Lab
      Attribute:
        Attribute_Label: Sample #
        Attribute_Definition: Sample number. All transect samples for NBP 04-09, and samples without trailing letters in NBP 05-08, are from the underway pump system with intake at 4 m. Samples with letters in NBP 05-08 (highlighted in light blue) are as follows: B= bucket sample. C= CTD sample 4m.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 1
            Range_Domain_Maximum: 45
      Attribute:
        Attribute_Label: Local date &amp; time
        Attribute_Definition: Date and time at the sampling location
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 12/20/2004 7:22
            Range_Domain_Maximum: 08.11.05   19:26
            Attribute_Units_of_Measure: NBP 04-09: month/day/year HH:MM (24-hour clock)
NBP 05-08: day.month.year HH:MM (24-hour clock)
      Attribute:
        Attribute_Label: Julian Day
        Attribute_Definition: Day of year.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 300
            Range_Domain_Maximum: 362
      Attribute:
        Attribute_Label: time (GMT)
        Attribute_Definition: Time of sampling in Greenwich Mean Time.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 00:00
            Range_Domain_Maximum: 23:59
      Attribute:
        Attribute_Label: Decimal Lat
        Attribute_Definition: Latitude in decimal degrees
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: -76.510
            Range_Domain_Maximum: -49.014
            Attribute_Units_of_Measure: decimal degrees
      Attribute:
        Attribute_Label: Decimal Lon
        Attribute_Definition: Longitude in decimal degrees
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: -179.200
            Range_Domain_Maximum: 179.818
            Attribute_Units_of_Measure: decimal degrees
      Attribute:
        Attribute_Label: Salinity
        Attribute_Definition: Salinity
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 33.504
            Range_Domain_Maximum: 34.598
      Attribute:
        Attribute_Label: Ext. SW Temp
        Attribute_Definition: Water temperature
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: -1.869
            Range_Domain_Maximum: 8.680
            Attribute_Units_of_Measure: Celsius
      Attribute:
        Attribute_Label: Fluor V
        Attribute_Definition: Fluorescence. Data were collected with a flow through fluorometer (Turner Designs).
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 2.920
            Range_Domain_Maximum: 63.200
            Attribute_Units_of_Measure: voltage
      Attribute:
        Attribute_Label: Beam C
        Attribute_Definition: Beam attenuation. Calculated as Beam C = -1/x * ln(Trans %) where x is the path length (25 cm according to NBP Data report).
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.001
            Range_Domain_Maximum: 0.012
            Attribute_Units_of_Measure: percent per centimeter
      Attribute:
        Attribute_Label: pCO2
        Attribute_Definition: Partial pressure of carbon dioxide.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 245
            Range_Domain_Maximum: 427.9
            Attribute_Units_of_Measure: parts per million
      Attribute:
        Attribute_Label: DMS [nM]
        Attribute_Definition: Concentration of dimethylsulfide. Samples were collected from the ship underway system by attaching a 0.2 um PolyCap Nylong filter capsule to the pump outflow and filling a 60 ml teflon bottle with minimal gas exchange.  These filtered samples were analyzed by purge and trap gas chromatography within 1 h of collection.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.383
            Range_Domain_Maximum: 30.244
            Attribute_Units_of_Measure: nanomol
      Attribute:
        Attribute_Label: Stdev DMS
        Attribute_Definition: Standard deviation for concentration of dimethylsulfide, as calculated for cruise NBP 05-08.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.009
            Range_Domain_Maximum: 0.882
            Attribute_Units_of_Measure: nanomol
      Attribute:
        Attribute_Label: DMS err
        Attribute_Definition: Standard error for concentration of dimethylsulfide, as calculated for cruise NBP 04-09.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.043
            Range_Domain_Maximum: 1.390
            Attribute_Units_of_Measure: nanomol
      Attribute:
        Attribute_Label: DMSPt or DMSP-tot [nM]
        Attribute_Definition: Total concentration of dimethylsulfoniopropionate. 

Ten ml of unfiltered seawater was collected directly from the pump outflow into 15 ml storage tube containing 50 ul of 50% H2SO4. Two replicate storage tubes per sample.   After &gt;12 h to allow DMS to oxidize, a 1.5 ml sub-sample from storage tube was pipetted into 14 ml serum vial and treated with 1 ml of 5 N NaOH.  Sample was purged entirely into cryotrop for quantification of DMS by FPD.  Corrections were made for Air-NaOH blanks which were low or absent during the cruise. 

Note: the acidification procedure may have resulted in loss of DMSP in samples containing colonial Phaeocystis sp.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 4.040
            Range_Domain_Maximum: 143.470
            Attribute_Units_of_Measure: nanomol
      Attribute:
        Attribute_Label: Stdev DMSPt [nM]
        Attribute_Definition: Standard deviation for concentration of dimethylsulfoniopropionate, as calculated for cruise NBP 05-08.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.147
            Range_Domain_Maximum: 8.586
            Attribute_Units_of_Measure: nanomol
      Attribute:
        Attribute_Label: DMSP err
        Attribute_Definition: Standard error of concentration for dimethylsulfoniopropionate, as calculated for cruise NBP 04-09.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0
            Range_Domain_Maximum: 9.021
            Attribute_Units_of_Measure: nanomol
      Attribute:
        Attribute_Label: DMSPd [nM]
        Attribute_Definition: Concentration of dissolved dimethylsulfoniopropionate. 

Samples for DMSP were collected by Niskin bottle on CTD casts to 4 m and subjected to the gentle, small volume drip filtration procedure described in Kiene &amp; Slezak (2006).  Briefly, unfiltered water samples were collected from Niskins directly into Plastic Gelman magnetic filter tower. About 50 ml of water was allowed to fill the tower, but only the first 3.5 ml of filtrate was collected into a 15 ml centrifuge tube that already contained 50 ul of 50% H2SO4.  Filter towers were rinsed with Q water and thorougly dried between each use. Analysis was as for DMSP-total only that 3 ml of sample were analyzed.  For more details, see: Kiene, R.P., Slezak, D., 2006. Low dissolved DMSP concentrations in seawater revealed by small volume gravity filtration and dialysis sampling. Limnol. Oceanog. Methods 4, 80-95. 

Occasionally, samples from the underway pump system were also collected by the small volume drip filtration procedure for comparison to the Niskin collections.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.382
            Range_Domain_Maximum: 4.188
            Attribute_Units_of_Measure: nanomol
      Attribute:
        Attribute_Label: Stdev DMSPd
        Attribute_Definition: Standard deviation for concentration of dissolved dimethylsulfoniopropionate.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.021
            Range_Domain_Maximum: 0.501
            Attribute_Units_of_Measure: nanomol
      Attribute:
        Attribute_Label: DMSOd [nM]
        Attribute_Definition: Concentration of dimethyl sulfoxide. 

Samples were collected from the ship underway system by attaching a 0.2 um PolyCap Nylong filter capsule to the pump outflow and filling a 60 ml teflon bottle. The samples were then frozen for later analysis. After thawing the samples were sparged to remove any pre-existing DMS, and then a sub-sample of 1 ml was treated with 0.3 ml of Acros TiCl3 (30%) and the vial immediately capped with a teflon faced septum. After capping with a new teflon faced septum, vials were heated at 50 deg C for 60 minutes.   After cooling to room temperature, the samples were analyzed by sparging the entire sample into the cryotrap. Samples were corrected for TiCl3 blanks.  Q water on the NBP was an inappropriate blank because it had some DMSO in it.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0
            Range_Domain_Maximum: 53.618
            Attribute_Units_of_Measure: nanomol
      Attribute:
        Attribute_Label: Stdev DMSOd
        Attribute_Definition: Standard deviation for concentration of dimethyl sulfoxide, as calculated for cruise NBP 05-08.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.007
            Range_Domain_Maximum: 0.555
            Attribute_Units_of_Measure: nanomol
      Attribute:
        Attribute_Label: DMSO err
        Attribute_Definition: Standard error for concentration of dimethyl sulfoxide, as calculated for cruise NBP 04-09.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Unrepresentable_Domain: This column is blank.
      Attribute:
        Attribute_Label: DMS/DMSPt
        Attribute_Definition: Concentration of dimethylsulfide divided by concentration of total dimethylsulfoniopropionate.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.031
            Range_Domain_Maximum: 0.380
      Attribute:
        Attribute_Label: notes
        Attribute_Definition: Miscellaneous notes about the sample or station.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Unrepresentable_Domain: Additional information in any form.
    Detailed_Description:
      Entity_Type:
        Entity_Type_Label: NBP Depth Profiles DMS-DMSP-DMSO concentrations and rates
        Entity_Type_Definition: Summary data for depth profiles of DMS, DMSP, and DMSO concentrations and fluxes, collected from CTD casts with Niskin bottles to depths up to 737 m. The data are organized into two MS Excel spreadsheets, one for each cruise.
        Entity_Type_Definition_Source: DISL: Kiene Lab
      Attribute:
        Attribute_Label: Station Information
        Attribute_Definition: Information includes station name, time of cast in GMT, and cast name.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Unrepresentable_Domain: Information includes station name, time of cast in GMT, and cast name.
      Attribute:
        Attribute_Label: Local date &amp; time
        Attribute_Definition: Date and time at the sampling location
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 12/20/2004 7:22
            Range_Domain_Maximum: 08.11.05   19:26
            Attribute_Units_of_Measure: NBP 04-09: month/day/year HH:MM (24-hour clock) NBP 05-08: day.month.year HH:MM (24-hour clock)
      Attribute:
        Attribute_Label: Decimal Lat
        Attribute_Definition: Latitude in decimal degrees
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: -76.510
            Range_Domain_Maximum: -49.014
            Attribute_Units_of_Measure: decimal degrees
      Attribute:
        Attribute_Label: Decimal Lon
        Attribute_Definition: Longitude in decimal degrees
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: -179.200
            Range_Domain_Maximum: 179.818
            Attribute_Units_of_Measure: decimal degrees
      Attribute:
        Attribute_Label: Depth (m)
        Attribute_Definition: Depth below water surface where the sample was collected.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 1
            Range_Domain_Maximum: 737
            Attribute_Units_of_Measure: meter
      Attribute:
        Attribute_Label: Plot depth
        Attribute_Definition: Negative depth of sample collection. For making quick depth column figures in Excel with the surface at the top.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: -737
            Range_Domain_Maximum: -1
            Attribute_Units_of_Measure: meter
      Attribute:
        Attribute_Label: DMS [nM]
        Attribute_Definition: Concentration of dimethylsulfide.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: -0.04
            Range_Domain_Maximum: 70.425
            Attribute_Units_of_Measure: nanomol
      Attribute:
        Attribute_Label: DMSPt [nM]
        Attribute_Definition: Total concentration of dimethylsulfoniopropionate.   Ten ml of unfiltered seawater was collected directly from Niskin into 15 ml storage tube containing 50 ul of 50% H2SO4. Two replicate storage tubes per depth, both from the same Niskin.   After &gt;12 h to allow DMS to oxidize, a 1.5 ml sub-sample from storage tube was pipetted into 14 ml serum vial and treated with 1 ml of 5 N NaOH.  Sample was purged entirely into cryotrop for quantification of DMS by FPD.  Corrections were made for Air-NaOH blanks which were low or absent during the cruise. Note: the acidification procedure may have resulted in loss of DMSP in samples containing colonial Phaeocystis sp.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0
            Range_Domain_Maximum: 86.059
            Attribute_Units_of_Measure: nanomol
      Attribute:
        Attribute_Label: DMSPd [nM]
        Attribute_Definition: Concentration of dissolved dimethylsulfoniopropionate.   Unfiltered water samples were collected from Niskins directly into Plastic Gelman magnetic filter tower. About 50 ml of water was allowed to fill the tower, but only the first 3.5 ml of filtrate was collected into a 15 ml centrifuge tube that already contained 50 ul of 50% H2SO4.  Filter towers were rinsed with Q water and thorougly dried between each use.     Analysis was as for DMSP-total except that 3 ml of sample were analyzed.  For more details, see: Kiene, R.P., Slezak, D., 2006. Low dissolved DMSP concentrations in seawater revealed by small volume gravity filtration and dialysis sampling. Limnol. Oceanog. Methods 4, 80-95.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.08
            Range_Domain_Maximum: 5.415
            Attribute_Units_of_Measure: nanomol
      Attribute:
        Attribute_Label: DMSPp (total - dissolved) [nM]
        Attribute_Definition: Particulate dimethylsulfoniopropionate was calculated as the difference between DMSP-total and DMSPd.  Error was propagated.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.57
            Range_Domain_Maximum: 85.376
            Attribute_Units_of_Measure: nanomol
      Attribute:
        Attribute_Label: DMSOd [nM]
        Attribute_Definition: Concentration of dissolved dimethyl sulfoxide. Whole water was drip filtered through muffled GF/F into 15 ml centri tubes (&lt; 8 ml collected). The samples were then frozen for later analysis. After thawing the samples were sparged to remove any pre-existing DMS, and then a sub-sample of 1 ml was treated with 0.3 ml of Acros TiCl3 (30%) and the vial immediately capped with a teflon faced septum. After capping with a new teflon faced septum, vials were heated at 50 deg C for 60 minutes.   After cooling to room temperature, the samples were analyzed by sparging the entire sample into the cryotrap. Samples were corrected for TiCl3 blanks.  Q water on the NBP was an inappropriate blank because it had some DMSO in it.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.042
            Range_Domain_Maximum: 60.596
            Attribute_Units_of_Measure: nanomol
      Attribute:
        Attribute_Label: Chl a (ug/L)
        Attribute_Definition: Chlorophyll a. Water samples were filtered onto either GF/F or AE glass fiber filters and extracted in 90% acetone for 24 h at -20 deg C.  Fluorescence was determined based on the acidification method.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.0006419
            Range_Domain_Maximum: 8.46505625
            Attribute_Units_of_Measure: micrograms per liter
      Attribute:
        Attribute_Label: Leucine Incorporation (nM/day)
        Attribute_Definition: Bacterial biomass production (Leucine Incorporation) - the uptake rate of 3H-Leucine is reported for samples treated with 20 nM Leucine and incubated for 1-4 h in the dark.  Leucine is incorporated into bacterial proteins. For more details see: Smith, D.C., Azam, F., 1992. A simple, economical method for measuring bacterial protein synthesis rates in sea water using 3H-Leucine. Marine Microbial Food Webs 6, 107-114.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0
            Range_Domain_Maximum: 1.4347
            Attribute_Units_of_Measure: nanomol per day
      Attribute:
        Attribute_Label: DMS-bio rate const (per day)
        Attribute_Definition: Biological turnover rate of dimethylsulfide. 35S-tracer methods were used to obtain the consumption rate constant, k (the fraction of DMS consumed per unit time).  Tracer incubations lasted 4 - 8 h and were conducted in the dark at the in situ temperature.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.0299
            Range_Domain_Maximum: 6.165
            Attribute_Units_of_Measure: per day
      Attribute:
        Attribute_Label: DMS bio rate (nM DMS/day)
        Attribute_Definition: Biological turnover rate of dimethylsulfide. 35S-tracer methods were used to obtain the consumption rate constant, k (the fraction of DMS consumed per unit time).  Tracer incubations lasted 4 - 8 h and were conducted in the dark at the in situ temperature.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.008
            Range_Domain_Maximum: 13.089
            Attribute_Units_of_Measure: nanomol per day
      Attribute:
        Attribute_Label: DMSPd rate const (per day)
        Attribute_Definition: Biological turnover rate of dissolved dimethylsulfoniopropionate. 35S-tracer methods were used to obtain the consumption rate constant, k (the fraction of DMSPd consumed per unit time).  Tracer incubations lasted 4 - 8 h and were conducted in the dark at the in situ temperature.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0.0487
            Range_Domain_Maximum: 14.178
            Attribute_Units_of_Measure: per day
      Attribute:
        Attribute_Label: DMSPd turnover rate (nM/day)
        Attribute_Definition: Biological turnover rate of dissolved dimethylsulfoniopropionate. 35S-tracer methods were used to obtain the consumption rate constant, k (the fraction of DMSPd consumed per unit time).  Tracer incubations lasted 4 - 8 h and were conducted in the dark at the in situ temperature.
        Attribute_Definition_Source: DISL: Kiene Lab
        Attribute_Domain_Values:
          Range_Domain:
            Range_Domain_Minimum: 0
            Range_Domain_Maximum: 16.37
            Attribute_Units_of_Measure: nanomol per day
  Metadata_Reference_Information:
    Metadata_Date: 20131210
    Metadata_Contact:
      Contact_Information:
        Contact_Organization_Primary:
          Contact_Organization: Dauphin Island Sea Lab
        Contact_Position: Data Management Specialist
        Contact_Address:
          Address_Type: mailing and physical
          Address: 101 Bienville Blvd.
          City: Dauphin Island
          State_or_Province: Al
          Postal_Code: 36528
          Country: USA
        Contact_Voice_Telephone: 251-861-2141
        Contact_Electronic_Mail_Address: metadata@disl.org
        Hours_of_Service: 8-5:00 CST
        Contact_Instructions: Please email the metadata specialist for further information.
    Metadata_Standard_Name: FGDC Content Standard for Digital Geospatial Metadata
    Metadata_Standard_Version: FGDC-STD-001.1-1999
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