			
											         01/31/05

Explanation regarding roi project data files submitted to NOAA 

We would like to submit to the NOAA data center all data that has come out of the HCRI funded project Feeding biology of the introduced fish roi (Cephalopholis argus), and its impact on Hawaiian reef fishes and fisheries between January 2004 and January 2005. 
The compiled data is currently located in four separate files. It is our goal to incorporate all files into a single Access database as soon as possible. As work on the integrated database progresses, and as additional data becomes available from the roi project, we will submit updates to the data that we are submitting at this time.


Files submitted:

The following files currently contain the output from the roi project:

1. The file Roi project database NOAA 1-05 in Access database format. This file contains the majority of all data that has come out of the roi project, in particular
- all information pertaining to the sample collection from the field, including parameter describing the sampled roi individuals (such as Length, Weight, sex, etc.).
- all information about the sample sites where roi were collected.
- all data from the Ciguatera analysis performed on the roi sample.
- all data from the Otolith analysis performed on the sample.

2. The file Stomach content analysis 1-15-05 in Excel format.

3. The file Feeding experiment 1-15-05 in Excel format.

4. The file Digestion experiment 1-15-05 in Excel format.


File explanations:

The column headings and parameters of all four files will be summarized in the following paragraphs to allow meaningful interpretation of the data.


1. Roi project database NOAA 1-05 (Access database)

The database contains the two tables Roi and sample sites. The columns occurring in each of the tables are listed below, together with a brief explanation of the data occurring in the respective columns.

- Sample sites:

Column header
Explanation/comments regarding data content of column
ID
Unique database identifier of each site
Site name
Name of site (official)
Alternative site name
Additional site names commonly used
Island
Island where site is located
Site location information
Additional site information for repeat visits of sites
Position comment
Comment regarding position fix of site (e.g., if position was not taken
Latitude degrees N
Position fix of site
Latitude minutes N
See above
Longitude degrees W
See above
Longitude minutes W
See above
Site access
Self explanatory
Site description
General impression of dive site
Depth max
Max. depth reached during dive
Depth average
Avg. depth of dive
Roi abundance (perception)
Self explanatory
Sampling date
Self explanatory
Sampling time
Self explanatory
# caught
Number of roi caught by entire team during dive
Remarks
Remarks regarding sampling effort
Divers
Divers by name
# of divers spearing
Of these divers, actively spearing
Dive time
Average dive time of all divers
Catch per unit effort
Not calculated yet, future work
Comments
General comments


- Roi:

Column header
Explanation/comments regarding data content of column
Fish
Unique database fish identifier number
Alt
Alternative number from original sampling book
Comments Ciguatera sample
Comments regarding the samples that were used for Ciguatera analysis (e.g., lost sample)
Column header (cont.)
Explanation (cont.)
Comments age analysis
Comments regarding Otolith analysis
Origin
Island origin of the sample (Oahu or Big Island)
Tray
Refers to storage of samples after collection
Position
Refers to storage of samples after collection
Species
Roi (original collection was also for taape)
Standard length (cm)
Self explanatory
Forklength
Self explanatory
Weight (g)
Self explanatory
Sex
Self explanatory
Maturity
Mature: gonads contain egg or sperm
Age (years)
Self explanatory
Gen. taken?
Identifier for genetic samples taken by researchers from the Smithsonian Institute, not relevant for this project
Date collected
Self explanatory
Site
Collection site
Remarks
Comments regarding sample
Regurgitation
Did the fish regurgitate food during collection?
Ciguatera, date tested
Test of Ciguatera test with MIA test
Solution date
MIA solution, date of production
Run by
Name of person running MIA test
Ciguatera remarks
General remarks regarding test of the sample
Head (Jan reading)
Three tests were run per fish, one with tissue from the head of the fish, one from the belly, and one from the tail (Name of person interpreting the test result in parentheses).
Belly (Jan)
See above
Tail (Jan)
See above
Score (Jan)
The three tests run per fish are added up to a total Ciguatera score
Head (reading Courtney)
Second interpretation of tests from above, conducted to analyze observer consistency
Belly (Courtney)

Tail (Courtney)
...
Score (Courtney)

Retest date
Second test run on a piece of tissue previously tested, conducted to analyze replicability of results
Solution date retest
See above
Retest run by
See above
Retest remarks
See above
Head (retest)
See above
Belly (retest)
See above
Tail (retest)
See above
Score (retest)
See above



2. Stomach content analysis 1-15-05 (Excel)

This file contains all data from the stomach content analysis performed on the Kona and Oahu roi samples. The Excel file contains the three worksheets Oahu (results from the Oahu sample, Kona (results from the Kona sample), and Analysis (summary and synthesis of some of the data from the Oahu and Kona worksheets). Terminology on the analysis worksheet is self-explanatory, therefore only the column headers from the other two worksheets are explained below.

- Oahu and Kona:

Column header
Explanation/comments regarding data content of column
Fish #
Unique fish ID-number (same as in Access file), links this data to other tables and the Access database
Comments sample
General comments regarding sample, e.g., sample missing
Regurgitation?
Did the sampled roi regurgitate prey during capture?
Full/Empty
Stomach full or empty?
Content
General classification of full stomachs: Fish, Crustacean or Goo (if only indiscernible mass)
# of items
How many distinct prey items found in stomach?
Content comment
Comments regarding stomach content
Family
If ID-able to family level, enter family here
Species
If ID-able to species level, enter species name here
Goo description
Quantification of amount of goo
Goo classification
Translation of previous column into one of 5 categories (see key below the Oahu table
Digestion
How digested is the prey item?
Digestion class
Classification of strength of digestion (five-step scale), see key below Oahu table
IDable?
At time of first opening of stomach, does item in stomach look ID-able or not?
Other comments
Any other comments regarding this row?



3. Tank feeding experiment 1-15-05 (Excel format)

This file contains the four worksheets Fish information (information about the sample of live roi used in the tank feeding and digestion experiments), Feeding data (contains all logged data on daily feeding of each individual roi in tank systems between 6/17/04 and 7/21/04), Analysis (contains a synthesis of data from the feeding log to calculate daily and long-term food consumption rates by captive roi), and Decomposition experiment (shows data from a side-experiment to analyze the rate of decomposition of roi food in the tanks without roi influence).


- Fish information:

Column header
Explanation/comments regarding data content of column
Fish
Fish identifier number for the live roi sample (not connected to sample obtained by spearfishing
TL
Total length of individuals (in cm)
SL
Standard length (cm)
Weight
Weight of individuals (wet weight in g)


-Feeding data:

Column header
Explanation/comments regarding data content of column
Date
Date of feeding
Temperature
Temperature in tanks
Tank
Tank indentifier (tanks were labeled 1 till 12)
Fish
Fish identifier to link to fish information worksheet
W consumed 
Weight of food consumed (g)
Pieces consumed
Pieces of food consumed
W t0
Initial weight of food put into tank
W t1
Weight of food remaining in tank after feeding
Pieces in dish
Initial number of pieces prepared for specific tank/fish
Pieces consumed directly
Pieces of food observed as eaten during feeding
Pieces left (additional)
Pieces left in tank after roi stopped feeding
P left next feeding
Pieces still left at time of next feeding
Observations
Any sort of observation regarding experiment  fish health, behavior, etc.
Notes
Mark specific experiments

-Analysis:

Self-explanatory, same terminology as in Feeding data used in analysis of food consumption rates over time.

-Decomposition experiment:

Column header
Explanation/comments regarding data content of column
Date
Date of experiment
Temp.
Temperature in tanks (degree Celsius)
Tank
Tank identifier
Pieces
Number of pieces in experiment
Shape
General shape/size of pieces
W change
Change in weight over time through decomposition

4. Digestion experiment 1-15-05 (Excel)

This file contains five worksheet, Factsheet, Analysis sheet, Interval summary, W-V ratio prey, and Balance test. Of the five, the last three are self-explanatory.

-Factsheet:

Column header
Explanation/comments regarding data content of column
Date feeding
Day of feeding
Time feeding
Time that roi swallowed offered prey item (Parrotfish)
Date sacrifice
Day of sacrifice of roi
Time sacrifice
Time of sacrifice
Planned interval
Originally planned interval of digestion for individual
Fish
Roi identifier to link to other tank experiments
TL
Roi total length (cm)
SL
Roi standard length (cm)
True Weight
Roi weight excluding weight of prey item that was in stomach
Weight
Measured weight at time of sacrifice
Prey
Is prey remaining in roi stomach at time of weighing or not (some items were removed immediately after sacrifice, or regurgitated by roi during sacrifice)?
Parrotfish W start 
Prey item weight before digestion interval
Parrotfish W finish
Prey item weight after digestion interval
Remarks
Any remarks regarding experiment


-Analysis sheet:

Column header
Explanation/comments regarding data content of column
Fish
Fish identifier to tie to other tank experiments and files
W prey/W roi
Prey consumed in terms of roi body weight
Digestion interval (h)
Total interval of prey item spending in roi stomach
Absolute W digested
Weight digested (g)
Proportionate W digested
Proportion of prey item that was digested during  digestion interval
Proportionate W remaining
Proportion of prey item remaining after digestion interval
Remarks
Any remarks regarding experiment/analysis

Feeding biology of the introduced fish roi (Cephalopholis argus), and its impact on Hawaiian reef fishes and fisheries (HCRI 2003-2004)        Birkeland, Walsh and Dierking




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