{"_index":"metadata_v1","_type":"_doc","_id":"gov.noaa.nodc:0309778","_version":98,"_seq_no":90432090,"_primary_term":205,"_ignored":["processStep.keyword"],"found":true,"_source":{ "src_source_type_s": "WAF", "src_source_uri_s": "WAF:https://data.noaa.gov/waf/NOAA/NESDIS/ncei/accessions/iso/xml/", "src_source_name_s": "NCEI-Accessions", "src_uri_s": "https://data.noaa.gov/waf/NOAA/NESDIS/ncei/accessions/iso/xml/0309778.xml", "src_lastupdate_dt": "2026-06-12T00:36:28Z", "src_source_ref_s": "b405284fdbcb42788bf94e755ca24d01", "src_task_ref_s": "93eb30b75e5544c1a8a52b262a5f3575", "sys_created_dt": "2025-11-27T16:19:34.659Z", "sys_modified_dt": "2026-06-13T19:13:15.757Z", "sys_xmlmodified_dt": "2026-06-12T18:07:05.434Z", "sys_owner_s": "NCEI", "sys_owner_txt": "NCEI", "sys_metadatatype_s": "iso19115-2", "sys_xml_clob": "\n \n gov.noaa.nodc:0309778\n \n \n eng\n \n \n utf8\n \n \n nonGeographicDataset\n \n \n \n \n NOAA National Centers for Environmental Information\n \n \n \n \n \n \n ncei.info@noaa.gov\n \n \n \n \n \n \n https://www.ncei.noaa.gov/contact\n \n \n HTTPS\n \n \n NOAA Contact Information\n \n \n Information for contacts at NCEI.\n \n \n information\n \n \n \n \n \n \n custodian\n \n \n \n \n 2026-05-06T12:33:59Z\n \n \n ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data\n \n \n ISO 19115-2:2009(E)\n \n \n \n \n \n \n Ontogenetic and environmental responses in metabolic enzyme activity of Pacific Arctic larval gadids from laboratory experiment studies from 2022-04-13 to 2023-06-28 (NCEI Accession 0309778)\n \n \n \n \n 2025-12-02\n \n \n publication\n \n \n \n \n \n \n 2025-11-24\n \n \n creation\n \n \n \n \n \n \n \n \n \n NOAA National Centers for Environmental Information\n \n \n \n \n \n gov.noaa.nodc:0309778\n \n \n \n \n \n \n \n \n NCEI Archive Management System\n \n \n \n \n \n 0309778\n \n \n \n \n \n \n NOAA National Centers for Environmental Information\n \n \n \n \n \n \n ncei.info@noaa.gov\n \n \n \n \n \n \n https://www.ncei.noaa.gov/contact\n \n \n HTTPS\n \n \n NCEI Contact Information\n \n \n Information for contacts at NCEI.\n \n \n information\n \n \n \n \n \n \n publisher\n \n \n \n \n \n \n Mara L. Spencer\n \n \n NOAA Alaska Fisheries Science Center (NMFS/AFSC)\n \n \n \n \n \n \n Mara.Spencer@noaa.gov\n \n \n \n \n \n \n http://www.afsc.noaa.gov\n \n \n HTTP\n \n \n NOAA Alaska Fisheries Science Center (NMFS/AFSC) website\n \n \n Institution web page\n \n \n information\n \n \n \n \n \n \n resourceProvider\n \n \n \n \n \n \n NOAA Alaska Fisheries Science Center (NMFS/AFSC)\n \n \n \n \n \n \n http://www.afsc.noaa.gov\n \n \n HTTP\n \n \n NOAA Alaska Fisheries Science Center (NMFS/AFSC) website\n \n \n Institution web page\n \n \n information\n \n \n \n \n \n \n resourceProvider\n \n \n \n \n \n \n Emily Slesinger\n \n \n NOAA Alaska Fisheries Science Center (NMFS/AFSC)\n \n \n \n \n \n \n emily.slesinger@noaa.gov\n \n \n \n \n \n \n http://www.afsc.noaa.gov\n \n \n HTTP\n \n \n NOAA Alaska Fisheries Science Center (NMFS/AFSC) website\n \n \n Institution web page\n \n \n information\n \n \n \n \n \n \n principalInvestigator\n \n \n \n \n \n \n Erik V. Thuesen\n \n \n The Evergreen State College\n \n \n \n \n \n \n https://www.evergreen.edu/\n \n \n HTTPS\n \n \n The Evergreen State College website\n \n \n Institution web page\n \n \n information\n \n \n \n \n \n \n principalInvestigator\n \n \n \n \n \n \n Thomas Hurst\n \n \n NOAA Alaska Fisheries Science Center (NMFS/AFSC)\n \n \n \n \n \n \n Thomas.Hurst@noaa.gov\n \n \n \n \n \n \n http://www.afsc.noaa.gov\n \n \n HTTP\n \n \n NOAA Alaska Fisheries Science Center (NMFS/AFSC) website\n \n \n Institution web page\n \n \n information\n \n \n \n \n \n \n principalInvestigator\n \n \n \n \n \n \n Slesinger, Emily\n \n \n NOAA Alaska Fisheries Science Center (NMFS/AFSC)\n \n \n \n \n \n \n http://www.afsc.noaa.gov\n \n \n HTTP\n \n \n NOAA Alaska Fisheries Science Center (NMFS/AFSC) website\n \n \n Institution web page\n \n \n information\n \n \n \n \n \n \n author\n \n \n \n \n \n \n Thuesen, Erik V.\n \n \n The Evergreen State College\n \n \n \n \n \n \n https://www.evergreen.edu/\n \n \n HTTPS\n \n \n The Evergreen State College website\n \n \n Institution web page\n \n \n information\n \n \n \n \n \n \n author\n \n \n \n \n \n \n Hurst, Thomas P.\n \n \n NOAA Alaska Fisheries Science Center (NMFS/AFSC)\n \n \n \n \n \n \n http://www.afsc.noaa.gov\n \n \n HTTP\n \n \n NOAA Alaska Fisheries Science Center (NMFS/AFSC) website\n \n \n Institution web page\n \n \n information\n \n \n \n \n \n \n author\n \n \n \n \n \n \n NOAA Alaska Fisheries Science Center (NMFS/AFSC)\n \n \n \n \n \n \n http://www.afsc.noaa.gov\n \n \n HTTP\n \n \n NOAA Alaska Fisheries Science Center (NMFS/AFSC) website\n \n \n Institution web page\n \n \n information\n \n \n \n \n \n \n contributor\n \n \n \n \n \n \n The Evergreen State College\n \n \n \n \n \n \n https://www.evergreen.edu/\n \n \n HTTPS\n \n \n The Evergreen State College website\n \n \n Institution web page\n \n \n information\n \n \n \n \n \n \n contributor\n \n \n \n \n tableDigital\n \n \n \n \n This dataset contains laboratory experiment data that were collected to examine potential effects of warming and ocean acidification in high latitude marine ecosystems. Warming in high latitude marine ecosystems is leading to the borealization of Arctic communities. Species-specific responses to temperature provide insight into potential co-occurrence or competitive advantage between Arctic and boreal species. Ocean acidification may also lead to unique species-specific responses. At the Pacific-Arctic interface, larval distributions of the boreal Pacific cod (Gadus macrocephalus) are increasingly overlapping with those of Arctic cod (Boreogadus saida). We assessed larval metabolic capacities by measuring metabolic enzyme activities of citrate synthase (CS; aerobic metabolism), lactate dehydrogenase (LDH; anaerobic metabolism), and β-hydroxyacyl CoA dehydrogenase (HOAD; fatty acid metabolism). Throughout early development, Pacific cod enzyme activities, including glycolytic capacity, were higher, and fatty acid metabolism lower than Arctic cod enzyme activities. These responses may reflect a more active larval lifestyle of Pacific cod. Separately, larvae were reared in multiple temperatures (Pacific cod: 3, 6, 10°C; Arctic cod 1.8, 5, 7.3°C) and pCO2 levels (ambient = ~350 μatm; high = ~1500 μatm). At the cold temperature, Pacific cod enzyme activities were higher than at the control temperature, indicating they were acclimating but less cold adapted than Arctic cod. Arctic cod HOAD activity and LDH:CS ratio were elevated under warmer temperatures suggesting increased energy demand. Elevated pCO2 levels only affected larvae at their control temperature and resulted in decreased Pacific cod HOAD activity and increased Arctic cod CS and HOAD activities. This indicates differing sensitivities to ocean acidification between the species. Overall, Pacific cod may continue to be constrained in their northern habitat by cold temperatures, but under slight warming to optimal growing temperatures, Pacific cod will have competitive advantage over Arctic cod.\n \n \n This dataset is available to the public for a wide variety of uses including scientific research and analysis.\n \n \n Funding Information: NOAA’s Ocean Acidification Program (#20903 to Thomas P. Hurst)\n \n \n completed\n \n \n historicalArchive\n \n \n \n \n NOAA National Centers for Environmental Information\n \n \n \n \n \n \n ncei.info@noaa.gov\n \n \n \n \n \n \n https://www.ncei.noaa.gov/contact\n \n \n HTTPS\n \n \n NCEI Contact Information\n \n \n Information for contacts at NCEI.\n \n \n information\n \n \n \n \n \n \n pointOfContact\n \n \n \n \n \n \n asNeeded\n \n \n \n \n \n \n https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0309778\n \n \n Preview graphic\n \n \n PNG\n \n \n \n \n \n \n 0309778\n \n \n \n \n NCEI ACCESSION NUMBER\n \n \n \n \n 2025-11-21\n \n \n publication\n \n \n \n \n \n \n \n \n \n \n DISSOLVED INORGANIC CARBON (DIC)\n \n \n partial pressure of carbon dioxide - water\n \n \n pH\n \n \n SPECIES IDENTIFICATION\n \n \n SPECIES IDENTIFICATION - DRY WEIGHT\n \n \n total alkalinity\n \n \n WATER TEMPERATURE\n \n \n theme\n \n \n \n \n NODC DATA TYPES THESAURUS\n \n \n \n \n \n \n \n \n \n carbon dioxide (CO2) gas analyzer\n \n \n pH sensor\n \n \n temperature sensor\n \n \n titrator\n \n \n instrument\n \n \n \n \n NODC INSTRUMENT TYPES THESAURUS\n \n \n \n \n \n \n \n \n \n biological\n \n \n chemical\n \n \n laboratory experiments\n \n \n physical\n \n \n survey - biological\n \n \n theme\n \n \n \n \n NODC OBSERVATION TYPES THESAURUS\n \n \n \n \n \n \n \n \n \n NOAA Alaska Fisheries Science Center\n \n \n The Evergreen State College\n \n \n dataCentre\n \n \n \n \n NODC COLLECTING INSTITUTION NAMES THESAURUS\n \n \n \n \n \n \n \n \n \n NOAA Alaska Fisheries Science Center\n \n \n dataCentre\n \n \n \n \n NODC SUBMITTING INSTITUTION NAMES THESAURUS\n \n \n \n \n \n \n \n \n \n Ocean Carbon and Acidification Data System (OCADS)\n \n \n US DOC; NOAA; Office of Oceanic and Atmospheric Research; Ocean Acidification Program (OAP)\n \n \n project\n \n \n \n \n NODC PROJECT NAMES THESAURUS\n \n \n \n \n \n \n \n \n \n Arctic Ocean\n \n \n place\n \n \n \n \n NODC SEA AREA NAMES THESAURUS\n \n \n \n \n \n \n \n \n \n oceanography\n \n \n theme\n \n \n \n \n WMO_CategoryCode\n \n \n \n \n 2012-09-15\n \n \n publication\n \n \n \n \n \n \n \n \n \n \n DOC/NOAA/NMFS/AFSC > Alaska Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce\n \n \n dataCentre\n \n \n \n \n Global Change Master Directory (GCMD) Data Center Keywords\n \n \n \n \n 2026\n \n \n revision\n \n \n \n \n 24\n \n \n \n \n Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA)\n \n \n \n \n \n \n Greenbelt\n \n \n MD\n \n \n \n \n \n \n https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords\n \n \n HTTPS\n \n \n GCMD Keyword Forum Page\n \n \n Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords\n \n \n information\n \n \n \n \n \n \n custodian\n \n \n \n \n \n \n \n \n \n \n Ocean Acidification Program (OAP)\n \n \n Ocean Carbon and Acidification Data System (OCADS) Project\n \n \n project\n \n \n \n \n Ocean Acidification Search Keywords\n \n \n \n \n \n \n \n \n \n EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > ALKALINITY\n \n \n EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CARBON DIOXIDE\n \n \n EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > INORGANIC CARBON\n \n \n EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PH\n \n \n EARTH SCIENCE > OCEANS > OCEAN TEMPERATURE > WATER TEMPERATURE\n \n \n theme\n \n \n \n \n Global Change Master Directory (GCMD) Science Keywords\n \n \n \n \n 2026\n \n \n revision\n \n \n \n \n 24\n \n \n \n \n Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA)\n \n \n \n \n \n \n Greenbelt\n \n \n MD\n \n \n \n \n \n \n https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords\n \n \n HTTPS\n \n \n GCMD Keyword Forum Page\n \n \n Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords\n \n \n information\n \n \n \n \n \n \n custodian\n \n \n \n \n \n \n \n \n \n \n Laboratory experiment\n \n \n theme\n \n \n \n \n OCADS Study Type\n \n \n \n \n \n \n \n \n \n CO2_trmt\n \n \n CS_P\n \n \n CS_WW\n \n \n DIC\n \n \n DPH\n \n \n DW\n \n \n DW_CONC\n \n \n Date\n \n \n HOAD_CS\n \n \n HOAD_P\n \n \n HOAD_WW\n \n \n LDH_CS\n \n \n LDH_P\n \n \n LDH_WW\n \n \n LEN\n \n \n PROTEIN_CONC\n \n \n SPP\n \n \n TA\n \n \n TEMP_trmt\n \n \n pCO2\n \n \n pH\n \n \n theme\n \n \n \n \n Provider Variable Abbreviations\n \n \n \n \n \n \n \n \n \n CO2 ANALYZERS > CO2 ANALYZERS\n \n \n PH METERS > PH METERS\n \n \n TEMPERATURE SENSORS > TEMPERATURE SENSORS\n \n \n instrument\n \n \n \n \n Global Change Master Directory (GCMD) Instrument Keywords\n \n \n \n \n 2026\n \n \n revision\n \n \n \n \n 24\n \n \n \n \n Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA)\n \n \n \n \n \n \n Greenbelt\n \n \n MD\n \n \n \n \n \n \n https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords\n \n \n HTTPS\n \n \n GCMD Keyword Forum Page\n \n \n Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords\n \n \n information\n \n \n \n \n \n \n custodian\n \n \n \n \n \n \n \n \n \n \n OCEAN > ARCTIC OCEAN\n \n \n place\n \n \n \n \n Global Change Master Directory (GCMD) Location Keywords\n \n \n \n \n 2026\n \n \n revision\n \n \n \n \n 24\n \n \n \n \n Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA)\n \n \n \n \n \n \n Greenbelt\n \n \n MD\n \n \n \n \n \n \n https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords\n \n \n HTTPS\n \n \n GCMD Keyword Forum Page\n \n \n Global Change Master Directory (GCMD). 2025. GCMD Keywords, Version 21. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords\n \n \n information\n \n \n \n \n \n \n custodian\n \n \n \n \n \n \n \n \n \n \n NOAA AFSC Laboratory\n \n \n Newport\n \n \n Oregon\n \n \n place\n \n \n \n \n Provider Geographic Names\n \n \n \n \n \n \n \n \n \n accessLevel: public\n \n \n \n \n \n \n otherRestrictions\n \n \n Cite as: Slesinger, Emily; Thuesen, Erik V.; Hurst, Thomas P. (2025). Ontogenetic and environmental responses in metabolic enzyme activity of Pacific Arctic larval gadids from laboratory experiment studies from 2022-04-13 to 2023-06-28 (NCEI Accession 0309778). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0309778. Accessed [date].\n \n \n \n \n \n \n Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.\n \n \n \n \n \n \n otherRestrictions\n \n \n Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.\n \n \n \n \n \n \n license\n \n \n These data were produced by NOAA and are not subject to copyright protection in the United States. NOAA waives any potential copyright and related rights in these data worldwide through the Creative Commons Zero 1.0 Universal Public Domain Dedication (CC0-1.0).\n \n \n SPDX License: Creative Commons Zero v1.0 Universal (CC0-1.0)\n \n \n \n \n \n \n \n \n NOAA National Centers for Environmental Information (2022). Ocean Carbon and Acidification Data System (OCADS). NOAA National Centers for Environmental Information. https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system\n \n \n \n \n 2022-04\n \n \n publication\n \n \n \n \n \n \n \n \n \n \n \n https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system\n \n \n HTTPS\n \n \n https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system\n \n \n OCADS website\n \n \n information\n \n \n \n \n \n \n \n \n \n \n \n NOAA National Centers for Environmental Information\n \n \n author\n \n \n \n \n \n \n NOAA National Centers for Environmental Information (NCEI)\n \n \n publisher\n \n \n \n \n documentDigital\n \n \n [accessed 2024-02-08]\n \n \n \n \n crossReference\n \n \n program\n \n \n \n \n \n \n \n \n Slesinger, Emily; Tyler, Erin F.; Mundorff, Samantha; Youn, Kathryn R.; Edmondson, Leo; Hurst, Thomas P. (2026). Data collected from laboratory experiments on the tolerance of egg and yolk-sac larval yellowfin sole (Limanda aspera) to ocean warming and acidification from 2022-07-01 to 2024-08-31 (NCEI Accession 0313059). NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.25921/cvec-sa43.\n \n \n \n \n 2026-03-06\n \n \n publication\n \n \n \n \n \n \n \n \n International DOI Foundation (IDF)\n \n \n \n \n \n https://doi.org/10.25921/cvec-sa43\n \n \n \n \n \n \n \n \n NOAA National Centers for Environmental Information\n \n \n \n \n \n gov.noaa.nodc:0313059\n \n \n \n \n \n \n \n \n \n \n \n https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0313059.html\n \n \n HTTPS\n \n \n https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0313059.html\n \n \n Related dataset\n \n \n information\n \n \n \n \n \n \n \n \n \n \n \n Slesinger, Emily\n \n \n NOAA Alaska Fisheries Science Center (NMFS/AFSC)\n \n \n \n \n \n \n http://www.afsc.noaa.gov\n \n \n HTTP\n \n \n NOAA Alaska Fisheries Science Center (NMFS/AFSC) website\n \n \n Institution web page\n \n \n information\n \n \n \n \n \n \n author\n \n \n \n \n \n \n Tyler, Erin F.\n \n \n Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS)\n \n \n \n \n \n \n https://cimers.oregonstate.edu/\n \n \n HTTPS\n \n \n Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS) website\n \n \n Institution web page\n \n \n information\n \n \n \n \n \n \n author\n \n \n \n \n \n \n Mundorff, Samantha\n \n \n Northeastern University\n \n \n \n \n \n \n http://www.northeastern.edu\n \n \n HTTP\n \n \n Northeastern University website\n \n \n Institution web page\n \n \n information\n \n \n \n \n \n \n author\n \n \n \n \n \n \n Youn, Kathryn R.\n \n \n Hendrix College\n \n \n author\n \n \n \n \n \n \n Edmondson, Leo\n \n \n University of New England\n \n \n author\n \n \n \n \n \n \n Hurst, Thomas P.\n \n \n NOAA Alaska Fisheries Science Center (NMFS/AFSC)\n \n \n \n \n \n \n http://www.afsc.noaa.gov\n \n \n HTTP\n \n \n NOAA Alaska Fisheries Science Center (NMFS/AFSC) website\n \n \n Institution web page\n \n \n information\n \n \n \n \n \n \n author\n \n \n \n \n \n \n NOAA National Centers for Environmental Information (NCEI)\n \n \n publisher\n \n \n \n \n tableDigital\n \n \n \n \n crossReference\n \n \n project\n \n \n \n \n eng; USA\n \n \n utf8\n \n \n environment\n \n \n oceans\n \n \n biota\n \n \n \n \n \n \n -124.043955\n \n \n -124.043955\n \n \n 44.621316\n \n \n 44.621316\n \n \n \n \n \n \n \n 2022-04-13\n 2023-06-28\n \n \n \n \n \n \n \n This data package (Submission ID: BJKHBMF0Y) was acquired by NCEI from the Scientific Data Integration System (SDIS) at the NOAA Pacific Marine Environmental Laboratory (PMEL) in accordance with the archival submission agreement between NCEI and PMEL.\n \n \n \n \n \n \n \n \n \n \n NOAA National Centers for Environmental Information\n \n \n \n \n \n \n +1-301-713-3277\n \n \n \n \n \n \n ncei.info@noaa.gov\n \n \n \n \n \n \n pointOfContact\n \n \n \n \n \n \n In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.\n \n \n Contact NCEI for other distribution options and instructions.\n \n \n \n \n \n \n Originator data format\n \n \n \n \n \n \n \n 0.108\n \n \n \n \n https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0309778.html\n \n \n HTTPS\n \n \n Web browser\n \n \n Project Metadata\n \n \n Navigate directly to the URL for a descriptive web page with download links.\n \n \n information\n \n \n \n \n \n \n https://www.ncei.noaa.gov/archive/accession/0309778\n \n \n HTTPS\n \n \n Web browser\n \n \n NCEI Dataset Landing Page\n \n \n Navigate directly to the URL for a descriptive web page with download links.\n \n \n information\n \n \n \n \n \n \n https://www.ncei.noaa.gov/archive/accession/oas/309778\n \n \n HTTPS\n \n \n Web browser\n \n \n Descriptive Information\n \n \n Navigate directly to the URL for a descriptive web page with download links.\n \n \n information\n \n \n \n \n \n \n https://www.ncei.noaa.gov/archive/accession/download/309778\n \n \n HTTPS\n \n \n Web browser\n \n \n HTTPS\n \n \n Navigate directly to the URL for data access and direct download.\n \n \n download\n \n \n \n \n \n \n ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0237/0309778/\n \n \n FTP\n \n \n Any FTP client\n \n \n FTP\n \n \n These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP).\n \n \n download\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n dataset\n \n \n \n \n \n \n \n \n NCEI Accession 0309778 v1.1 was published.\n \n \n 2025-12-02T18:11:33Z\n \n \n \n \n \n \n NCEI Accession 0309778 v1.1\n \n \n \n \n \n publication\n \n \n \n \n \n \n \n \n \n \n \n https://www.ncei.noaa.gov/archive/accession/0309778/1.1\n \n \n HTTPS\n \n \n NCEI Accession 0309778 v1.1\n \n \n published 2025-12-02T18:11:33Z\n \n \n download\n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n \n nonGeographicDataset\n \n \n \n \n \n \n \n \n Parameter or Variable: Dissolved Inorganic Carbon;\nAbbreviation: DIC;\nUnit: μmol/kg;\nObservation type: Laboratory experiment;\nIn-situ / Manipulation / Response variable: MANIPULATIONCONDITION;\nMeasured or calculated: MEASURED;\nSampling instrument: Siphoning tube;\nAnalyzing instrument: AIRICA (Automated InfraRed Inorganic Carbon Analyzer);\nPreservation method: saturated HgCl2;\nPreservative correction: no correction;\nUncertainty: <0.05%;\nResearcher name: Natalie Monacci;\nResearcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks\n \n \n \n \n \n \n Parameter or Variable: Total alkalinity;\nAbbreviation: TA;\nUnit: μmol/kg;\nObservation type: Laboratory experiment;\nIn-situ / Manipulation / Response variable: MANIPULATIONCONDITION;\nMeasured or calculated: MEASURED;\nSampling instrument: Siphoning tube;\nAnalyzing instrument: VINDTA 3C (Versatile Instrument for the Determination of dissolved inorganic carbon and Total Alkalinity);\nCRM manufacturer: Andrew Dickson's lab at Scripps Institute of Oceanography;\nPreservation method: saturated HgCl2;\nPreservative correction: no correction;\nUncertainty: <0.05%;\nResearcher name: Natalie Monacci;\nResearcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks\n \n \n \n \n \n \n Parameter or Variable: pH;\nAbbreviation: pH;\npH scale: SEAWATERSCALE(SWS);\nIn-situ / Manipulation / Response variable: MANIPULATIONCONDITION;\nMeasured or calculated: CALCULATED;\nDetailed sampling and analyzing information: calculated from DIC, TA, temperature, and salinity;\nAt what temperature was pH reported: 5.9±0.4°C;\nMethod reference: Lewis and Wallace 1995. Basic programs for the CO2 system in seawater. Brookhaven National Laboratory. BNL-61827;\nResearcher name: Natalie Monacci;\nResearcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks\n \n \n \n \n \n \n Parameter or Variable: pCO2 (fCO2) autonomous;\nAbbreviation: pCO2;\nUnit: µatm;\nObservation type: Laboratory experiment;\nIn-situ / Manipulation / Response variable: MANIPULATIONCONDITION;\nMeasured or calculated: CALCULATED;\nDetailed sampling and analyzing information: calculated from DIC, TA, temperature, and salinity;\nMethod reference: Lewis and Wallace 1995. Basic programs for the CO2 system in seawater. Brookhaven National Laboratory. BNL-61827;\nResearcher name: Natalie Monacci;\nResearcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks\n \n \n \n \n \n \n Parameter or Variable: Species identification;\nAbbreviation: SPP;\nUnit: none;\nDetailed sampling and analyzing information: Coded as \"Acod\" for Arctic cod, \"Pcod\" for Pacific cod.;\nBiological subject: Arctic cod, Pacific cod;\nSpecies ID: Boreogadus saida, Gadus macrocephalus\n \n \n \n \n \n \n Parameter or Variable: CO2 treatment;\nAbbreviation: CO2_trmt;\nUnit: none;\nDetailed sampling and analyzing information: Coded as \"amb\" for ambient level or \"high\" for high treatment level. A Durafet III pH probe (Honeywell) in each of the high pCO2 treatment header tanks regulated the injection of CO2 based on achieving temperature-dependent pH targets (see Slesinger et al., 2024).\n \n \n \n \n \n \n Parameter or Variable: Water temperature treatment designation;\nAbbreviation: TEMP_trmt;\nUnit: °C;\nObservation type: Laboratory experiment;\nDetailed sampling and analyzing information: Coded as “1.8”, “5”, or “7.3” for the targeted treatment temperatures during the experiment.\n \n \n \n \n \n \n Parameter or Variable: lactate dehydrogenase to citrate synthase ratio;\nAbbreviation: LDH_CS;\nUnit: none;\nObservation type: Laboratory experiment;\nIn-situ / Manipulation / Response variable: RESPONSEVARIABLE;\nMeasured or calculated: CALCULATED;\nBiological subject: Arctic cod, Pacific cod;\nSpecies ID: Boreogadus saida, Gadus macrocephalus;\nResearcher name: Emily Slesinger;\nResearcher institution: NOAA Alaska Fisheries Science Center\n \n \n \n \n \n \n Parameter or Variable: β-hydroxyacyl CoA dehydrogenase to citrate synthase ratio;\nAbbreviation: HOAD_CS;\nUnit: none;\nObservation type: Laboratory experiment;\nIn-situ / Manipulation / Response variable: RESPONSEVARIABLE;\nMeasured or calculated: CALCULATED;\nBiological subject: Arctic cod, Pacific cod;\nSpecies ID: Boreogadus saida, Gadus macrocephalus;\nResearcher name: Emily Slesinger;\nResearcher institution: NOAA Alaska Fisheries Science Center\n \n \n \n \n \n \n Parameter or Variable: citrate synthase protein;\nAbbreviation: CS_P;\nUnit: mg protein;\nObservation type: Laboratory experiment;\nIn-situ / Manipulation / Response variable: RESPONSEVARIABLE;\nMeasured or calculated: MEASURED;\nAnalyzing instrument: Spectrophotometer (Genesys 150 UV-Vis, ThermoFisher);\nDetailed sampling and analyzing information: Maximum activities (Vmax) of CS, LDH and HOAD were measured spectrophotometrically (Genesys 150 UV-Vis, ThermoFisher) at 20°C in 1-mL cuvettes under non-limiting conditions. All assays were run in triplicate and the intra-assay variation (CV) was < 10% for each enzyme. CS activity was assayed in 50 mM Imidazole buffer (pH 7.8), 1.5 mM MgCl2, 0.1 mM DTNB, and 0.1 mM acetyl-CoA. Once the homogenate was added, background activity was measured, and then the reaction was initiated by adding 25 μL oxaloacetic acid for a final concentration of 1 mM. Activity was measured for 5 minutes at 412 nm, using an extinction coefficient of 13.6 OD μmol-1 mL-1 to calculate activity rates. LDH and HOAD activities were measured at 340 nm for 2 minutes and activity rates were calculated using an extinction coefficient of 6.22 OD μmol-1 mL-1. LDH was assayed in 80 mM Imidazole buffer (pH 7.2), 100 mM KCl, 5 mM pyruvate, and 0.15 mM NADH. HOAD was assayed in 50 mM Imidazole buffer (pH 7.4), 100 mM KCl, 0.15 mM NADH, and 0.1 mM acetoacetyl CoA.;\nMethod reference: Thuesen EV, Miller CB, Childress JJ (1998) Ecophysiological interpretation of oxygen consumption rates and enzymatic activities of deep-sea copepods. Mar Ecol Prog Ser 168:95-107.;\nBiological subject: Arctic cod, Pacific cod;\nSpecies ID: Boreogadus saida, Gadus macrocephalus;\nResearcher name: Emily Slesinger;\nResearcher institution: NOAA Alaska Fisheries Science Center\n \n \n \n \n \n \n Parameter or Variable: β-hydroxyacyl CoA dehydrogenase protein;\nAbbreviation: HOAD_P;\nUnit: mg protein;\nObservation type: Laboratory experiment;\nIn-situ / Manipulation / Response variable: RESPONSEVARIABLE;\nMeasured or calculated: MEASURED;\nAnalyzing instrument: Spectrophotometer (Genesys 150 UV-Vis, ThermoFisher);\nDetailed sampling and analyzing information: Maximum activities (Vmax) of CS, LDH and HOAD were measured spectrophotometrically (Genesys 150 UV-Vis, ThermoFisher) at 20°C in 1-mL cuvettes under non-limiting conditions. All assays were run in triplicate and the intra-assay variation (CV) was < 10% for each enzyme. CS activity was assayed in 50 mM Imidazole buffer (pH 7.8), 1.5 mM MgCl2, 0.1 mM DTNB, and 0.1 mM acetyl-CoA. Once the homogenate was added, background activity was measured, and then the reaction was initiated by adding 25 μL oxaloacetic acid for a final concentration of 1 mM. Activity was measured for 5 minutes at 412 nm, using an extinction coefficient of 13.6 OD μmol-1 mL-1 to calculate activity rates. LDH and HOAD activities were measured at 340 nm for 2 minutes and activity rates were calculated using an extinction coefficient of 6.22 OD μmol-1 mL-1. LDH was assayed in 80 mM Imidazole buffer (pH 7.2), 100 mM KCl, 5 mM pyruvate, and 0.15 mM NADH. HOAD was assayed in 50 mM Imidazole buffer (pH 7.4), 100 mM KCl, 0.15 mM NADH, and 0.1 mM acetoacetyl CoA.;\nMethod reference: Singer TD, Ballantyne JS (1989) Absence of extraheptic lipid oxidation in a freshwater elasmobranch, the dwarf stingray Potamotrygon magdalenae: evidence from enzyme activities. J Exp Zool 251:355-360.;\nBiological subject: Arctic cod, Pacific cod;\nSpecies ID: Boreogadus saida, Gadus macrocephalus;\nResearcher name: Emily Slesinger;\nResearcher institution: NOAA Alaska Fisheries Science Center\n \n \n \n \n \n \n Parameter or Variable: lactate dehydrogenase protein;\nAbbreviation: LDH_P;\nUnit: mg protein;\nObservation type: Laboratory experiment;\nIn-situ / Manipulation / Response variable: RESPONSEVARIABLE;\nMeasured or calculated: MEASURED;\nAnalyzing instrument: Spectrophotometer (Genesys 150 UV-Vis, ThermoFisher);\nDetailed sampling and analyzing information: Maximum activities (Vmax) of CS, LDH and HOAD were measured spectrophotometrically (Genesys 150 UV-Vis, ThermoFisher) at 20°C in 1-mL cuvettes under non-limiting conditions. All assays were run in triplicate and the intra-assay variation (CV) was < 10% for each enzyme. CS activity was assayed in 50 mM Imidazole buffer (pH 7.8), 1.5 mM MgCl2, 0.1 mM DTNB, and 0.1 mM acetyl-CoA. Once the homogenate was added, background activity was measured, and then the reaction was initiated by adding 25 μL oxaloacetic acid for a final concentration of 1 mM. Activity was measured for 5 minutes at 412 nm, using an extinction coefficient of 13.6 OD μmol-1 mL-1 to calculate activity rates. LDH and HOAD activities were measured at 340 nm for 2 minutes and activity rates were calculated using an extinction coefficient of 6.22 OD μmol-1 mL-1. LDH was assayed in 80 mM Imidazole buffer (pH 7.2), 100 mM KCl, 5 mM pyruvate, and 0.15 mM NADH. HOAD was assayed in 50 mM Imidazole buffer (pH 7.4), 100 mM KCl, 0.15 mM NADH, and 0.1 mM acetoacetyl CoA.;\nMethod reference: Thuesen EV, Miller CB, Childress JJ (1998) Ecophysiological interpretation of oxygen consumption rates and enzymatic activities of deep-sea copepods. Mar Ecol Prog Ser 168:95-107.;\nBiological subject: Arctic cod, Pacific cod;\nSpecies ID: Boreogadus saida, Gadus macrocephalus;\nResearcher name: Emily Slesinger;\nResearcher institution: NOAA Alaska Fisheries Science Center\n \n \n \n \n \n \n Parameter or Variable: citrate synthase wet-weight;\nAbbreviation: CS_WW;\nUnit: mg wet-weight;\nObservation type: Laboratory experiment;\nAnalyzing instrument: Spectrophotometer (Genesys 150 UV-Vis, ThermoFisher);\nDetailed sampling and analyzing information: Maximum activities (Vmax) of CS, LDH and HOAD were measured spectrophotometrically (Genesys 150 UV-Vis, ThermoFisher) at 20°C in 1-mL cuvettes under non-limiting conditions. All assays were run in triplicate and the intra-assay variation (CV) was < 10% for each enzyme. CS activity was assayed in 50 mM Imidazole buffer (pH 7.8), 1.5 mM MgCl2, 0.1 mM DTNB, and 0.1 mM acetyl-CoA. Once the homogenate was added, background activity was measured, and then the reaction was initiated by adding 25 μL oxaloacetic acid for a final concentration of 1 mM. Activity was measured for 5 minutes at 412 nm, using an extinction coefficient of 13.6 OD μmol-1 mL-1 to calculate activity rates. LDH and HOAD activities were measured at 340 nm for 2 minutes and activity rates were calculated using an extinction coefficient of 6.22 OD μmol-1 mL-1. LDH was assayed in 80 mM Imidazole buffer (pH 7.2), 100 mM KCl, 5 mM pyruvate, and 0.15 mM NADH. HOAD was assayed in 50 mM Imidazole buffer (pH 7.4), 100 mM KCl, 0.15 mM NADH, and 0.1 mM acetoacetyl CoA.;\nMethod reference: Thuesen EV, Miller CB, Childress JJ (1998) Ecophysiological interpretation of oxygen consumption rates and enzymatic activities of deep-sea copepods. Mar Ecol Prog Ser 168:95-107.;\nBiological subject: Arctic cod, Pacific cod;\nSpecies ID: Boreogadus saida, Gadus macrocephalus;\nResearcher name: Emily Slesinger;\nResearcher institution: NOAA Alaska Fisheries Science Center\n \n \n \n \n \n \n Parameter or Variable: β-hydroxyacyl CoA dehydrogenase wet-weight;\nAbbreviation: HOAD_WW;\nUnit: mg wet-weight;\nObservation type: Laboratory experiment;\nIn-situ / Manipulation / Response variable: RESPONSEVARIABLE;\nMeasured or calculated: MEASURED;\nAnalyzing instrument: Spectrophotometer (Genesys 150 UV-Vis, ThermoFisher);\nDetailed sampling and analyzing information: Maximum activities (Vmax) of CS, LDH and HOAD were measured spectrophotometrically (Genesys 150 UV-Vis, ThermoFisher) at 20°C in 1-mL cuvettes under non-limiting conditions. All assays were run in triplicate and the intra-assay variation (CV) was < 10% for each enzyme. CS activity was assayed in 50 mM Imidazole buffer (pH 7.8), 1.5 mM MgCl2, 0.1 mM DTNB, and 0.1 mM acetyl-CoA. Once the homogenate was added, background activity was measured, and then the reaction was initiated by adding 25 μL oxaloacetic acid for a final concentration of 1 mM. Activity was measured for 5 minutes at 412 nm, using an extinction coefficient of 13.6 OD μmol-1 mL-1 to calculate activity rates. LDH and HOAD activities were measured at 340 nm for 2 minutes and activity rates were calculated using an extinction coefficient of 6.22 OD μmol-1 mL-1. LDH was assayed in 80 mM Imidazole buffer (pH 7.2), 100 mM KCl, 5 mM pyruvate, and 0.15 mM NADH. HOAD was assayed in 50 mM Imidazole buffer (pH 7.4), 100 mM KCl, 0.15 mM NADH, and 0.1 mM acetoacetyl CoA.;\nMethod reference: Singer TD, Ballantyne JS (1989) Absence of extraheptic lipid oxidation in a freshwater elasmobranch, the dwarf stingray Potamotrygon magdalenae: evidence from enzyme activities. J Exp Zool 251:355-360.;\nBiological subject: Arctic cod, Pacific cod;\nSpecies ID: Boreogadus saida, Gadus macrocephalus;\nResearcher name: Emily Slesinger;\nResearcher institution: NOAA Alaska Fisheries Science Center\n \n \n \n \n \n \n Parameter or Variable: lactate dehydrogenase wet-weight;\nAbbreviation: LDH_WW;\nUnit: mg wet-weight;\nObservation type: Laboratory experiment;\nIn-situ / Manipulation / Response variable: RESPONSEVARIABLE;\nMeasured or calculated: MEASURED;\nAnalyzing instrument: Spectrophotometer (Genesys 150 UV-Vis, ThermoFisher);\nDetailed sampling and analyzing information: Maximum activities (Vmax) of CS, LDH and HOAD were measured spectrophotometrically (Genesys 150 UV-Vis, ThermoFisher) at 20°C in 1-mL cuvettes under non-limiting conditions. All assays were run in triplicate and the intra-assay variation (CV) was < 10% for each enzyme. CS activity was assayed in 50 mM Imidazole buffer (pH 7.8), 1.5 mM MgCl2, 0.1 mM DTNB, and 0.1 mM acetyl-CoA. Once the homogenate was added, background activity was measured, and then the reaction was initiated by adding 25 μL oxaloacetic acid for a final concentration of 1 mM. Activity was measured for 5 minutes at 412 nm, using an extinction coefficient of 13.6 OD μmol-1 mL-1 to calculate activity rates. LDH and HOAD activities were measured at 340 nm for 2 minutes and activity rates were calculated using an extinction coefficient of 6.22 OD μmol-1 mL-1. LDH was assayed in 80 mM Imidazole buffer (pH 7.2), 100 mM KCl, 5 mM pyruvate, and 0.15 mM NADH. HOAD was assayed in 50 mM Imidazole buffer (pH 7.4), 100 mM KCl, 0.15 mM NADH, and 0.1 mM acetoacetyl CoA.;\nMethod reference: Thuesen EV, Miller CB, Childress JJ (1998) Ecophysiological interpretation of oxygen consumption rates and enzymatic activities of deep-sea copepods. Mar Ecol Prog Ser 168:95-107.;\nBiological subject: Arctic cod, Pacific cod;\nSpecies ID: Boreogadus saida, Gadus macrocephalus;\nResearcher name: Emily Slesinger;\nResearcher institution: NOAA Alaska Fisheries Science Center\n \n \n \n \n \n \n Parameter or Variable: Larval age in days post hatch;\nAbbreviation: DPH;\nUnit: days;\nObservation type: Laboratory experiment\n \n \n \n \n \n \n Parameter or Variable: Protein concentration;\nAbbreviation: PROTEIN_CONC;\nUnit: mg per mL;\nObservation type: Laboratory experiment;\nIn-situ / Manipulation / Response variable: RESPONSEVARIABLE;\nMeasured or calculated: MEASURED;\nAnalyzing instrument: Spectrophotometer (SpectraMax Plus 384, Molecular Devices);\nDetailed sampling and analyzing information: Protein concentrations (mg mL-1) were determined spectrophotometrically (SpectraMax Plus 384, Molecular Devices) at 37°C using a BCA kit (ThermoFisher).;\nMethod reference: https://documents.thermofisher.com/TFS-Assets/LSG/manuals/MAN0011430_Pierce_BCA_Protein_Asy_UG.pdf;\nBiological subject: Arctic cod, Pacific cod;\nSpecies ID: Boreogadus saida, Gadus macrocephalus;\nResearcher name: Emily Slesinger;\nResearcher institution: NOAA Alaska Fisheries Science Center\n \n \n \n \n \n \n Parameter or Variable: Sampling date;\nAbbreviation: Date;\nUnit: none\n \n \n \n \n \n \n Parameter or Variable: Mean dry weight;\nAbbreviation: DW;\nUnit: mg;\nObservation type: Laboratory experiment;\nIn-situ / Manipulation / Response variable: RESPONSEVARIABLE;\nMeasured or calculated: MEASURED;\nAnalyzing instrument: Sartorius Microbalance ME5;\nDetailed sampling and analyzing information: Fifteen larvae sampled from each tank were pooled into groups of three, rinsed with ammonium formate and dried at 60°C to a constant weight (DW; mg).;\nBiological subject: Arctic cod, Pacific cod;\nSpecies ID: Boreogadus saida, Gadus macrocephalus;\nResearcher name: Emily Slesinger;\nResearcher institution: NOAA Alaska Fisheries Science Center\n \n \n \n \n \n \n Parameter or Variable: Proportion of dry weight to wet weight;\nAbbreviation: DW_CONC;\nUnit: none;\nObservation type: l;\nIn-situ / Manipulation / Response variable: RESPONSEVARIABLE;\nMeasured or calculated: CALCULATED;\nAnalyzing instrument: Sartorius Microbalance ME5;\nDetailed sampling and analyzing information: Fifteen larvae sampled from each tank were pooled into groups of three, rinsed with ammonium formate and dried at 60°C to a constant weight (DW; mg).;\nBiological subject: Arctic cod, Pacific cod;\nSpecies ID: Boreogadus saida, Gadus macrocephalus;\nResearcher name: Emily Slesinger;\nResearcher institution: NOAA Alaska Fisheries Science Center\n \n \n \n \n \n \n Parameter or Variable: Standard length;\nAbbreviation: LEN;\nUnit: mm;\nObservation type: Laboratory experiment;\nIn-situ / Manipulation / Response variable: RESPONSEVARIABLE;\nMeasured or calculated: MEASURED;\nAnalyzing instrument: ImagePro image analysis system;\nDetailed sampling and analyzing information: Larvae were photographed using a dissecting microscope, and images were processed in ImageJ to measure standard length (LEN; mm).;\nBiological subject: Arctic cod, Pacific cod;\nSpecies ID: Boreogadus saida, Gadus macrocephalus;\nResearcher name: Emily Slesinger;\nResearcher institution: NOAA Alaska Fisheries Science Center\n \n \n \n \n \n \n \n \n \n \n asNeeded\n \n \n Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness.\n \n \n \n \n NOAA National Centers for Environmental Information\n \n \n custodian\n \n \n \n \n \n \n \n \n \n \n \n \n carbon dioxide (CO2) gas analyzer\n \n \n \n \n carbon dioxide (CO2) gas analyzer\n \n \n measures gaseous carbon dioxide\n\nCarbon dioxide gas analyzer has often been referred to as LI-COR, because a lot of researchers choose to use products manufactured by LI-COR incorporation for their carbon dioxide measurement. The main component of a carbon dioxide analyzer is a nondispersive infrared (NDIR) spectroscopic sensors to detect CO2 in a gaseous environment by its characteristic absorption.\n\nCarbon dioxide (CO2) gas analyzer works together with carbon dioxide (CO2) equilibrator (insttypes_id 184) to measure carbon dioxide in the water.\n\nTo measure water carbon dioxide, water needs to be continuously supplied to a showerhead inside an equilibrator. As the water flows through the equilibrator chamber, dissolved carbon dioxide exchanges quickly with air in the headspace. This air is then sampled by the carbon dioxide analyzer to determine the mole fraction of CO2 in water.\n \n \n \n \n \n \n \n \n pH sensor\n \n \n \n \n pH sensor\n \n \n generic meter used to measure pH\n\nAny of a class of instruments used to quantify pH in a water or other type of sample.\n \n \n \n \n \n \n \n \n temperature sensor\n \n \n \n \n temperature sensor\n \n \n A sensor used to measure temperature.\n \n \n \n \n \n \n \n \n titrator\n \n \n \n \n titrator\n \n \n titrator\n \n \n \n \n \n", "sys_xmlmeta_obj": { "mimetype": "application/xml", "hash": "5441848194230522422:347373905427328125:143840", "date": "2026-06-12T18:07:05.467Z", "size": 143840 }, "fileid": "gov.noaa.nodc:0309778", "title": "Ontogenetic and environmental responses in metabolic enzyme activity of Pacific Arctic larval gadids from laboratory experiment studies from 2022-04-13 to 2023-06-28 (NCEI Accession 0309778)", "description": "This dataset contains laboratory experiment data that were collected to examine potential effects of warming and ocean acidification in high latitude marine ecosystems. Warming in high latitude marine ecosystems is leading to the borealization of Arctic communities. Species-specific responses to temperature provide insight into potential co-occurrence or competitive advantage between Arctic and boreal species. Ocean acidification may also lead to unique species-specific responses. At the Pacific-Arctic interface, larval distributions of the boreal Pacific cod (Gadus macrocephalus) are increasingly overlapping with those of Arctic cod (Boreogadus saida). We assessed larval metabolic capacities by measuring metabolic enzyme activities of citrate synthase (CS; aerobic metabolism), lactate dehydrogenase (LDH; anaerobic metabolism), and β-hydroxyacyl CoA dehydrogenase (HOAD; fatty acid metabolism). Throughout early development, Pacific cod enzyme activities, including glycolytic capacity, were higher, and fatty acid metabolism lower than Arctic cod enzyme activities. These responses may reflect a more active larval lifestyle of Pacific cod. Separately, larvae were reared in multiple temperatures (Pacific cod: 3, 6, 10°C; Arctic cod 1.8, 5, 7.3°C) and pCO2 levels (ambient = ~350 μatm; high = ~1500 μatm). At the cold temperature, Pacific cod enzyme activities were higher than at the control temperature, indicating they were acclimating but less cold adapted than Arctic cod. Arctic cod HOAD activity and LDH:CS ratio were elevated under warmer temperatures suggesting increased energy demand. Elevated pCO2 levels only affected larvae at their control temperature and resulted in decreased Pacific cod HOAD activity and increased Arctic cod CS and HOAD activities. This indicates differing sensitivities to ocean acidification between the species. Overall, Pacific cod may continue to be constrained in their northern habitat by cold temperatures, but under slight warming to optimal growing temperatures, Pacific cod will have competitive advantage over Arctic cod.", "keywords_s": [ "0309778", "DISSOLVED INORGANIC CARBON (DIC)", "partial pressure of carbon dioxide - water", "pH", "SPECIES IDENTIFICATION", "SPECIES IDENTIFICATION - DRY WEIGHT", "total alkalinity", "WATER TEMPERATURE", "carbon dioxide (CO2) gas analyzer", "pH sensor", "temperature sensor", "titrator", "biological", "chemical", "laboratory experiments", "physical", "survey - biological", "NOAA Alaska Fisheries Science Center", "The Evergreen State College", "NOAA Alaska Fisheries Science Center", "Ocean Carbon and Acidification Data System (OCADS)", "US DOC; NOAA; Office of Oceanic and Atmospheric Research; Ocean Acidification Program (OAP)", "Arctic Ocean", "oceanography", "DOC/NOAA/NMFS/AFSC > Alaska Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce", "Ocean Acidification Program (OAP)", "Ocean Carbon and Acidification Data System (OCADS) Project", "EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > ALKALINITY", "EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CARBON DIOXIDE", "EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > INORGANIC CARBON", "EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PH", "EARTH SCIENCE > OCEANS > OCEAN TEMPERATURE > WATER TEMPERATURE", "Laboratory experiment", "CO2_trmt", "CS_P", "CS_WW", "DIC", "DPH", "DW", "DW_CONC", "Date", "HOAD_CS", "HOAD_P", "HOAD_WW", "LDH_CS", "LDH_P", "LDH_WW", "LEN", "PROTEIN_CONC", "SPP", "TA", "TEMP_trmt", "pCO2", "pH", "CO2 ANALYZERS > CO2 ANALYZERS", "PH METERS > PH METERS", "TEMPERATURE SENSORS > TEMPERATURE SENSORS", "OCEAN > ARCTIC OCEAN", "NOAA AFSC Laboratory", "Newport", "Oregon", "environment", "oceans", "biota" ], "thumbnail_s": "https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0309778", "contact_organizations_s": [ "NOAA National Centers for Environmental Information", "NOAA National Centers for Environmental Information", "NOAA Alaska Fisheries Science Center (NMFS/AFSC)", "NOAA Alaska Fisheries Science Center (NMFS/AFSC)", "NOAA Alaska Fisheries Science Center (NMFS/AFSC)", "The Evergreen State College", "NOAA Alaska Fisheries Science Center (NMFS/AFSC)", "NOAA Alaska Fisheries Science Center (NMFS/AFSC)", "The Evergreen State College", "NOAA Alaska Fisheries Science Center (NMFS/AFSC)", "NOAA Alaska Fisheries Science Center (NMFS/AFSC)", "The Evergreen State College", "NOAA National Centers for Environmental Information", "Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA)", "Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA)", "Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA)", "Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA)", "NOAA National Centers for Environmental Information", "NOAA National Centers for Environmental Information (NCEI)", "NOAA Alaska Fisheries Science Center (NMFS/AFSC)", "Cooperative Institute for Marine Ecosystem and Resource Studies (CIMERS)", "Northeastern University", "Hendrix College", "University of New England", "NOAA Alaska Fisheries Science Center (NMFS/AFSC)", "NOAA National Centers for Environmental Information (NCEI)", "NOAA National Centers for Environmental Information", "NOAA National Centers for Environmental Information" ], "contact_people_s": [ "Mara L. Spencer", "Emily Slesinger", "Erik V. Thuesen", "Thomas Hurst", "Slesinger, Emily", "Thuesen, Erik V.", "Hurst, Thomas P.", "Slesinger, Emily", "Tyler, Erin F.", "Mundorff, Samantha", "Youn, Kathryn R.", "Edmondson, Leo", "Hurst, Thomas P." ], "project_metadata": [ "https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0309778.html" ], "website.url": [ "https://www.ncei.noaa.gov/contact", "https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0309778.html", "https://www.ncei.noaa.gov/archive/accession/0309778", "https://www.ncei.noaa.gov/archive/accession/oas/309778" ], "dataThemeseanames_s": [ "Arctic Ocean" ], "dataThemeprojects_s": [ "Ocean Carbon and Acidification Data System (OCADS)", "US DOC; NOAA; Office of Oceanic and Atmospheric Research; Ocean Acidification Program (OAP)" ], "dataThemeinstitutions_s": [ "NOAA Alaska Fisheries Science Center", "The Evergreen State College", "NOAA Alaska Fisheries Science Center" ], "dataThemedatatypes_s": [ "DISSOLVED INORGANIC CARBON (DIC)", "partial pressure of carbon dioxide - water", "pH", "SPECIES IDENTIFICATION", "SPECIES IDENTIFICATION - DRY WEIGHT", "total alkalinity", "WATER TEMPERATURE" ], "dataThemeinstruments_s": [ "carbon dioxide (CO2) gas analyzer", "pH sensor", "temperature sensor", "titrator" ], "dataThemeobservations_s": [ "biological", "chemical", "laboratory experiments", "physical", "survey - biological" ], "people": [ "Mara L. Spencer", "Emily Slesinger", "Erik V. Thuesen", "Thomas Hurst", "Slesinger, Emily", "Thuesen, Erik V.", "Hurst, Thomas P." ], "nccos_research_priorities_and_topics_s": [ "Ocean Acidification Program (OAP)", "HOAD_CS", "HOAD_P", "HOAD_WW", "NOAA AFSC Laboratory" ], "supplementalInfo": "This data package (Submission ID: BJKHBMF0Y) was acquired by NCEI from the Scientific Data Integration System (SDIS) at the NOAA Pacific Marine Environmental Laboratory (PMEL) in accordance with the archival submission agreement between NCEI and PMEL.", "credit": "Funding Information: NOAA’s Ocean Acidification Program (#20903 to Thomas P. Hurst)", "purpose": "This dataset is available to the public for a wide variety of uses including scientific research and analysis.", "processStep": [ "NCEI Accession 0309778 v1.1 was published.", "Parameter or Variable: Dissolved Inorganic Carbon;\nAbbreviation: DIC;\nUnit: μmol/kg;\nObservation type: Laboratory experiment;\nIn-situ / Manipulation / Response variable: MANIPULATIONCONDITION;\nMeasured or calculated: MEASURED;\nSampling instrument: Siphoning tube;\nAnalyzing instrument: AIRICA (Automated InfraRed Inorganic Carbon Analyzer);\nPreservation method: saturated HgCl2;\nPreservative correction: no correction;\nUncertainty: HTML Markup Was Removed", "Parameter or Variable: Total alkalinity;\nAbbreviation: TA;\nUnit: μmol/kg;\nObservation type: Laboratory experiment;\nIn-situ / Manipulation / Response variable: MANIPULATIONCONDITION;\nMeasured or calculated: MEASURED;\nSampling instrument: Siphoning tube;\nAnalyzing instrument: VINDTA 3C (Versatile Instrument for the Determination of dissolved inorganic carbon and Total Alkalinity);\nCRM manufacturer: Andrew Dickson's lab at Scripps Institute of Oceanography;\nPreservation method: saturated HgCl2;\nPreservative correction: no correction;\nUncertainty: HTML Markup Was Removed", "Parameter or Variable: pH;\nAbbreviation: pH;\npH scale: SEAWATERSCALE(SWS);\nIn-situ / Manipulation / Response variable: MANIPULATIONCONDITION;\nMeasured or calculated: CALCULATED;\nDetailed sampling and analyzing information: calculated from DIC, TA, temperature, and salinity;\nAt what temperature was pH reported: 5.9±0.4°C;\nMethod reference: Lewis and Wallace 1995. Basic programs for the CO2 system in seawater. Brookhaven National Laboratory. BNL-61827;\nResearcher name: Natalie Monacci;\nResearcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks", "Parameter or Variable: pCO2 (fCO2) autonomous;\nAbbreviation: pCO2;\nUnit: µatm;\nObservation type: Laboratory experiment;\nIn-situ / Manipulation / Response variable: MANIPULATIONCONDITION;\nMeasured or calculated: CALCULATED;\nDetailed sampling and analyzing information: calculated from DIC, TA, temperature, and salinity;\nMethod reference: Lewis and Wallace 1995. Basic programs for the CO2 system in seawater. Brookhaven National Laboratory. BNL-61827;\nResearcher name: Natalie Monacci;\nResearcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks", "Parameter or Variable: Species identification;\nAbbreviation: SPP;\nUnit: none;\nDetailed sampling and analyzing information: Coded as \"Acod\" for Arctic cod, \"Pcod\" for Pacific cod.;\nBiological subject: Arctic cod, Pacific cod;\nSpecies ID: Boreogadus saida, Gadus macrocephalus", "Parameter or Variable: CO2 treatment;\nAbbreviation: CO2_trmt;\nUnit: none;\nDetailed sampling and analyzing information: Coded as \"amb\" for ambient level or \"high\" for high treatment level. A Durafet III pH probe (Honeywell) in each of the high pCO2 treatment header tanks regulated the injection of CO2 based on achieving temperature-dependent pH targets (see Slesinger et al., 2024).", "Parameter or Variable: Water temperature treatment designation;\nAbbreviation: TEMP_trmt;\nUnit: °C;\nObservation type: Laboratory experiment;\nDetailed sampling and analyzing information: Coded as “1.8”, “5”, or “7.3” for the targeted treatment temperatures during the experiment.", "Parameter or Variable: lactate dehydrogenase to citrate synthase ratio;\nAbbreviation: LDH_CS;\nUnit: none;\nObservation type: Laboratory experiment;\nIn-situ / Manipulation / Response variable: RESPONSEVARIABLE;\nMeasured or calculated: CALCULATED;\nBiological subject: Arctic cod, Pacific cod;\nSpecies ID: Boreogadus saida, Gadus macrocephalus;\nResearcher name: Emily Slesinger;\nResearcher institution: NOAA Alaska Fisheries Science Center", "Parameter or Variable: β-hydroxyacyl CoA dehydrogenase to citrate synthase ratio;\nAbbreviation: HOAD_CS;\nUnit: none;\nObservation type: Laboratory experiment;\nIn-situ / Manipulation / Response variable: RESPONSEVARIABLE;\nMeasured or calculated: CALCULATED;\nBiological subject: Arctic cod, Pacific cod;\nSpecies ID: Boreogadus saida, Gadus macrocephalus;\nResearcher name: Emily Slesinger;\nResearcher institution: NOAA Alaska Fisheries Science Center", "Parameter or Variable: citrate synthase protein;\nAbbreviation: CS_P;\nUnit: mg protein;\nObservation type: Laboratory experiment;\nIn-situ / Manipulation / Response variable: RESPONSEVARIABLE;\nMeasured or calculated: MEASURED;\nAnalyzing instrument: Spectrophotometer (Genesys 150 UV-Vis, ThermoFisher);\nDetailed sampling and analyzing information: Maximum activities (Vmax) of CS, LDH and HOAD were measured spectrophotometrically (Genesys 150 UV-Vis, ThermoFisher) at 20°C in 1-mL cuvettes under non-limiting conditions. All assays were run in triplicate and the intra-assay variation (CV) was HTML Markup Was Removed", "Parameter or Variable: β-hydroxyacyl CoA dehydrogenase protein;\nAbbreviation: HOAD_P;\nUnit: mg protein;\nObservation type: Laboratory experiment;\nIn-situ / Manipulation / Response variable: RESPONSEVARIABLE;\nMeasured or calculated: MEASURED;\nAnalyzing instrument: Spectrophotometer (Genesys 150 UV-Vis, ThermoFisher);\nDetailed sampling and analyzing information: Maximum activities (Vmax) of CS, LDH and HOAD were measured spectrophotometrically (Genesys 150 UV-Vis, ThermoFisher) at 20°C in 1-mL cuvettes under non-limiting conditions. All assays were run in triplicate and the intra-assay variation (CV) was HTML Markup Was Removed", "Parameter or Variable: lactate dehydrogenase protein;\nAbbreviation: LDH_P;\nUnit: mg protein;\nObservation type: Laboratory experiment;\nIn-situ / Manipulation / Response variable: RESPONSEVARIABLE;\nMeasured or calculated: MEASURED;\nAnalyzing instrument: Spectrophotometer (Genesys 150 UV-Vis, ThermoFisher);\nDetailed sampling and analyzing information: Maximum activities (Vmax) of CS, LDH and HOAD were measured spectrophotometrically (Genesys 150 UV-Vis, ThermoFisher) at 20°C in 1-mL cuvettes under non-limiting conditions. All assays were run in triplicate and the intra-assay variation (CV) was HTML Markup Was Removed", "Parameter or Variable: citrate synthase wet-weight;\nAbbreviation: CS_WW;\nUnit: mg wet-weight;\nObservation type: Laboratory experiment;\nAnalyzing instrument: Spectrophotometer (Genesys 150 UV-Vis, ThermoFisher);\nDetailed sampling and analyzing information: Maximum activities (Vmax) of CS, LDH and HOAD were measured spectrophotometrically (Genesys 150 UV-Vis, ThermoFisher) at 20°C in 1-mL cuvettes under non-limiting conditions. All assays were run in triplicate and the intra-assay variation (CV) was HTML Markup Was Removed", "Parameter or Variable: β-hydroxyacyl CoA dehydrogenase wet-weight;\nAbbreviation: HOAD_WW;\nUnit: mg wet-weight;\nObservation type: Laboratory experiment;\nIn-situ / Manipulation / Response variable: RESPONSEVARIABLE;\nMeasured or calculated: MEASURED;\nAnalyzing instrument: Spectrophotometer (Genesys 150 UV-Vis, ThermoFisher);\nDetailed sampling and analyzing information: Maximum activities (Vmax) of CS, LDH and HOAD were measured spectrophotometrically (Genesys 150 UV-Vis, ThermoFisher) at 20°C in 1-mL cuvettes under non-limiting conditions. All assays were run in triplicate and the intra-assay variation (CV) was HTML Markup Was Removed", "Parameter or Variable: lactate dehydrogenase wet-weight;\nAbbreviation: LDH_WW;\nUnit: mg wet-weight;\nObservation type: Laboratory experiment;\nIn-situ / Manipulation / Response variable: RESPONSEVARIABLE;\nMeasured or calculated: MEASURED;\nAnalyzing instrument: Spectrophotometer (Genesys 150 UV-Vis, ThermoFisher);\nDetailed sampling and analyzing information: Maximum activities (Vmax) of CS, LDH and HOAD were measured spectrophotometrically (Genesys 150 UV-Vis, ThermoFisher) at 20°C in 1-mL cuvettes under non-limiting conditions. All assays were run in triplicate and the intra-assay variation (CV) was HTML Markup Was Removed", "Parameter or Variable: Larval age in days post hatch;\nAbbreviation: DPH;\nUnit: days;\nObservation type: Laboratory experiment", "Parameter or Variable: Protein concentration;\nAbbreviation: PROTEIN_CONC;\nUnit: mg per mL;\nObservation type: Laboratory experiment;\nIn-situ / Manipulation / Response variable: RESPONSEVARIABLE;\nMeasured or calculated: MEASURED;\nAnalyzing instrument: Spectrophotometer (SpectraMax Plus 384, Molecular Devices);\nDetailed sampling and analyzing information: Protein concentrations (mg mL-1) were determined spectrophotometrically (SpectraMax Plus 384, Molecular Devices) at 37°C using a BCA kit (ThermoFisher).;\nMethod reference: https://documents.thermofisher.com/TFS-Assets/LSG/manuals/MAN0011430_Pierce_BCA_Protein_Asy_UG.pdf;\nBiological subject: Arctic cod, Pacific cod;\nSpecies ID: Boreogadus saida, Gadus macrocephalus;\nResearcher name: Emily Slesinger;\nResearcher institution: NOAA Alaska Fisheries Science Center", "Parameter or Variable: Sampling date;\nAbbreviation: Date;\nUnit: none", "Parameter or Variable: Mean dry weight;\nAbbreviation: DW;\nUnit: mg;\nObservation type: Laboratory experiment;\nIn-situ / Manipulation / Response variable: RESPONSEVARIABLE;\nMeasured or calculated: MEASURED;\nAnalyzing instrument: Sartorius Microbalance ME5;\nDetailed sampling and analyzing information: Fifteen larvae sampled from each tank were pooled into groups of three, rinsed with ammonium formate and dried at 60°C to a constant weight (DW; mg).;\nBiological subject: Arctic cod, Pacific cod;\nSpecies ID: Boreogadus saida, Gadus macrocephalus;\nResearcher name: Emily Slesinger;\nResearcher institution: NOAA Alaska Fisheries Science Center", "Parameter or Variable: Proportion of dry weight to wet weight;\nAbbreviation: DW_CONC;\nUnit: none;\nObservation type: l;\nIn-situ / Manipulation / Response variable: RESPONSEVARIABLE;\nMeasured or calculated: CALCULATED;\nAnalyzing instrument: Sartorius Microbalance ME5;\nDetailed sampling and analyzing information: Fifteen larvae sampled from each tank were pooled into groups of three, rinsed with ammonium formate and dried at 60°C to a constant weight (DW; mg).;\nBiological subject: Arctic cod, Pacific cod;\nSpecies ID: Boreogadus saida, Gadus macrocephalus;\nResearcher name: Emily Slesinger;\nResearcher institution: NOAA Alaska Fisheries Science Center", "Parameter or Variable: Standard length;\nAbbreviation: LEN;\nUnit: mm;\nObservation type: Laboratory experiment;\nIn-situ / Manipulation / Response variable: RESPONSEVARIABLE;\nMeasured or calculated: MEASURED;\nAnalyzing instrument: ImagePro image analysis system;\nDetailed sampling and analyzing information: Larvae were photographed using a dissecting microscope, and images were processed in ImageJ to measure standard length (LEN; mm).;\nBiological subject: Arctic cod, Pacific cod;\nSpecies ID: Boreogadus saida, Gadus macrocephalus;\nResearcher name: Emily Slesinger;\nResearcher institution: NOAA Alaska Fisheries Science Center" ], "cruise_id": "https://doi.org/10.25921/cvec-sa43", "links_s": [ "https://www.ncei.noaa.gov/contact", "https://www.ncei.noaa.gov/contact", "http://www.afsc.noaa.gov", "http://www.afsc.noaa.gov", "http://www.afsc.noaa.gov", "https://www.evergreen.edu/", "http://www.afsc.noaa.gov", "http://www.afsc.noaa.gov", "https://www.evergreen.edu/", "http://www.afsc.noaa.gov", "http://www.afsc.noaa.gov", "https://www.evergreen.edu/", "https://www.ncei.noaa.gov/contact", "https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system", "https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0313059.html", "http://www.afsc.noaa.gov", "https://cimers.oregonstate.edu/", "http://www.northeastern.edu", "http://www.afsc.noaa.gov", "https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0309778.html", "https://www.ncei.noaa.gov/archive/accession/0309778", "https://www.ncei.noaa.gov/archive/accession/oas/309778", "https://www.ncei.noaa.gov/archive/accession/download/309778", "ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0237/0309778/", "https://www.ncei.noaa.gov/archive/accession/0309778/1.1" ], "apiso_Identifier_s": "gov.noaa.nodc:0309778", "apiso_Title_txt": "Ontogenetic and environmental responses in metabolic enzyme activity of Pacific Arctic larval gadids from laboratory experiment studies from 2022-04-13 to 2023-06-28 (NCEI Accession 0309778)", "apiso_Abstract_txt": "This dataset contains laboratory experiment data that were collected to examine potential effects of warming and ocean acidification in high latitude marine ecosystems. Warming in high latitude marine ecosystems is leading to the borealization of Arctic communities. Species-specific responses to temperature provide insight into potential co-occurrence or competitive advantage between Arctic and boreal species. Ocean acidification may also lead to unique species-specific responses. At the Pacific-Arctic interface, larval distributions of the boreal Pacific cod (Gadus macrocephalus) are increasingly overlapping with those of Arctic cod (Boreogadus saida). We assessed larval metabolic capacities by measuring metabolic enzyme activities of citrate synthase (CS; aerobic metabolism), lactate dehydrogenase (LDH; anaerobic metabolism), and β-hydroxyacyl CoA dehydrogenase (HOAD; fatty acid metabolism). Throughout early development, Pacific cod enzyme activities, including glycolytic capacity, were higher, and fatty acid metabolism lower than Arctic cod enzyme activities. These responses may reflect a more active larval lifestyle of Pacific cod. Separately, larvae were reared in multiple temperatures (Pacific cod: 3, 6, 10°C; Arctic cod 1.8, 5, 7.3°C) and pCO2 levels (ambient = ~350 μatm; high = ~1500 μatm). At the cold temperature, Pacific cod enzyme activities were higher than at the control temperature, indicating they were acclimating but less cold adapted than Arctic cod. Arctic cod HOAD activity and LDH:CS ratio were elevated under warmer temperatures suggesting increased energy demand. Elevated pCO2 levels only affected larvae at their control temperature and resulted in decreased Pacific cod HOAD activity and increased Arctic cod CS and HOAD activities. This indicates differing sensitivities to ocean acidification between the species. Overall, Pacific cod may continue to be constrained in their northern habitat by cold temperatures, but under slight warming to optimal growing temperatures, Pacific cod will have competitive advantage over Arctic cod.", "apiso_BrowseGraphic_s": "https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0309778", "apiso_OrganizationName_txt": "NOAA National Centers for Environmental Information", "apiso_Subject_txt": [ "0309778", "DISSOLVED INORGANIC CARBON (DIC)", "partial pressure of carbon dioxide - water", "pH", "SPECIES IDENTIFICATION", "SPECIES IDENTIFICATION - DRY WEIGHT", "total alkalinity", "WATER TEMPERATURE", "carbon dioxide (CO2) gas analyzer", "pH sensor", "temperature sensor", "titrator", "biological", "chemical", "laboratory experiments", "physical", "survey - biological", "NOAA Alaska Fisheries Science Center", "The Evergreen State College", "NOAA Alaska Fisheries Science Center", "Ocean Carbon and Acidification Data System (OCADS)", "US DOC; NOAA; Office of Oceanic and Atmospheric Research; Ocean Acidification Program (OAP)", "Arctic Ocean", "oceanography", "DOC/NOAA/NMFS/AFSC > Alaska Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce", "Ocean Acidification Program (OAP)", "Ocean Carbon and Acidification Data System (OCADS) Project", "EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > ALKALINITY", "EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CARBON DIOXIDE", "EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > INORGANIC CARBON", "EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PH", "EARTH SCIENCE > OCEANS > OCEAN TEMPERATURE > WATER TEMPERATURE", "Laboratory experiment", "CO2_trmt", "CS_P", "CS_WW", "DIC", "DPH", "DW", "DW_CONC", "Date", "HOAD_CS", "HOAD_P", "HOAD_WW", "LDH_CS", "LDH_P", "LDH_WW", "LEN", "PROTEIN_CONC", "SPP", "TA", "TEMP_trmt", "pCO2", "pH", "CO2 ANALYZERS > CO2 ANALYZERS", "PH METERS > PH METERS", "TEMPERATURE SENSORS > TEMPERATURE SENSORS", "OCEAN > ARCTIC OCEAN", "NOAA AFSC Laboratory", "Newport", "Oregon", "environment", "oceans", "biota" ], "apiso_TopicCategory_s": [ "environment", "oceans", "biota" ], "apiso_KeywordType_s": [ "theme", "instrument", "theme", "dataCentre", "dataCentre", "project", "place", "theme", "dataCentre", "project", "theme", "theme", "theme", "instrument", "place", "place" ], "apiso_Type_s": "nonGeographicDataset", "apiso_Modified_dt": "2026-05-06T12:33:59Z", "apiso_CreationDate_dt": "2025-11-24T00:00:00Z", "apiso_PublicationDate_dt": "2025-12-02T00:00:00Z", "apiso_Language_s": "eng", "apiso_ResourceLanguage_s": "eng", "apiso_AccessConstraints_s": [ "accessLevel: public" ], "apiso_OtherConstraints_s": [ "Cite as: Slesinger, Emily; Thuesen, Erik V.; Hurst, Thomas P. (2025). Ontogenetic and environmental responses in metabolic enzyme activity of Pacific Arctic larval gadids from laboratory experiment studies from 2022-04-13 to 2023-06-28 (NCEI Accession 0309778). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0309778. Accessed [date].", "Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.", "These data were produced by NOAA and are not subject to copyright protection in the United States. NOAA waives any potential copyright and related rights in these data worldwide through the Creative Commons Zero 1.0 Universal Public Domain Dedication (CC0-1.0).", "SPDX License: Creative Commons Zero v1.0 Universal (CC0-1.0)" ], "apiso_HasSecurityConstraints_b": true, "envelope_geo": [ { "type": "envelope", "coordinates": [ [ -124.043955, 44.62131601 ], [ -124.04395499, 44.621316 ] ] } ], "envelope_cen_pt": [ { "lat": 44.621316, "lon": -124.043955 } ], "timeperiod_nst": [ { "begin_dt": "2022-04-13T00:00:00Z", "begin_year_l": "2022", "begin_indeterminate_s": null, "end_dt": "2023-06-28T23:59:59.999Z", "end_year_l": "2023", "end_indeterminate_s": null } ], "metadata_year_l": [ 2022 ], "apiso_ConditionApplyingToAccessAndUse_txt": [ "Cite as: Slesinger, Emily; Thuesen, Erik V.; Hurst, Thomas P. (2025). Ontogenetic and environmental responses in metabolic enzyme activity of Pacific Arctic larval gadids from laboratory experiment studies from 2022-04-13 to 2023-06-28 (NCEI Accession 0309778). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0309778. Accessed [date].", "Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose." ], "apiso_ResponsiblePartyRole_txt": "pointOfContact", "url_thumbnail_s": "https://www.ncei.noaa.gov/access/metadata/landing-page/bin/gfx?id=gov.noaa.nodc:0309778", "url_website_s": "https://www.ncei.noaa.gov/contact", "url_project_metadata_s": "https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0309778.html", "url_http_download_s": "https://www.ncei.noaa.gov/archive/accession/download/309778", "url_ftp_download_s": "ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0237/0309778/" }}