# Symbiont photosynthetic competency and its effect on boron proxies in planktic foraminifera, Puerto Rico, Spring 2010 and 2015, and Santa Catalina Island, Summer 2013 #----------------------------------------------------------------------- # World Data Service for Paleoclimatology, Boulder # and # NOAA Paleoclimatology Program #----------------------------------------------------------------------- # Template Version 3.0 # Encoding: UTF-8 # NOTE: Please cite original publication, online resource and date accessed when using this data. # If there is no publication information, please cite Investigator, title, online resource and date accessed. # # Description/Documentation lines begin with # # Data lines have no # # # Online_Resource: https://www.ncdc.noaa.gov/paleo/study/23151 # Description: NOAA Landing Page # Online_Resource: https://www.ncei.noaa.gov/pub/data/paleo/contributions_by_author/hoenisch2020/hoenisch2020-fluor_chlor.txt # Description: NOAA location of the template # # # Data_Type: Paleoceanography # # Dataset_DOI: # # Parameter_Keywords: physical properties #--------------------------------------- # Contribution_Date # Date: 2020-06-24 #--------------------------------------- # File_Last_Modified_Date # Date: 2020-06-24 #--------------------------------------- # Title # Study_Name: Symbiont photosynthetic competency and its effect on boron proxies in planktic foraminifera, Puerto Rico, Spring 2010 and 2015, and Santa Catalina Island, Summer 2013 #--------------------------------------- # Investigators # Investigators: Hönisch, Bärbel; Fish, Carina; Phelps, Samuel; Haynes, Laura; Dyez, Kelsey; Holland, Kate; Fehrenbacher, Jennifer; Allen, Katherine; Eggins, Stephen; Goes, Joaquim #--------------------------------------- # Description_Notes_and_Keywords # Description: Research conducted at the Marine Lab of the Universidad de Puerto Rico, La Parguera and Wrigley Institute of Environmental Studies, Santa Catalina Island, CA # Provided Keywords: symbiont photosynthesis, planktic foraminifera, B/Ca, boron isotopes, fluorescence, Chlorophyll a #--------------------------------------- # Publication # Authors: Hönisch, Bärbel, Carina R. Fish, Samuel Phelps, Laura L. Haynes, Kelsey Dyez, Kate Holland, Jennifer Fehrenbacher, Katherine A. Allen, Stephen M. Eggins, and Joaquim I. Goes # Published_Date_or_Year: 2020 # Published_Title: Symbiont photosynthetic competency and its effect on boron proxies in planktic foraminifera # Journal_Name: Paleoceanography and Paleoclimatology # Volume: # Edition: # Issue: # Pages: # Report_Number: # DOI: # Online_Resource: # Full_Citation: # Abstract: Boron proxies in the calcium carbonate shells of planktic foraminifera are sensitive to seawater acidity, but B/Ca ratios and isotopic composition (i.e. d11B) recorded by different foraminifer species grown under identical environmental conditions differ significantly and systematically. Specifically, the symbiont-bearing Globigerinoides ruber records higher B/Ca and d11B than Trilobatus sacculifer and Orbulina universa, and it has been hypothesized that these differences are caused by species-specific rates of symbiont photosynthesis and habitat depth. According to this hypothesis, greater symbiont photosynthesis elevates the microenvironmental pH of G. ruber to a greater degree than in T. sacculifer and O. universa. Here we test this hypothesis by applying fast repetition rate fluorometry (FRRF), Chlorophyll a quantification, and symbiont counts in laboratory grown specimens of G. ruber, T. sacculifer and O. universa to study species-specific differences in symbiont photosynthetic competency. In addition, we report B/Ca shell profiles measured by laser ablation on the same specimens previously monitored by FRRF, and d11B data of discrete populations of all three species grown under high and low light conditions in the laboratory. While the light experiments document that symbiont photosynthesis elevates pH and/or d11B in the calcifying microenvironment of all three foraminifer species, the FRRF, Chl. a and symbiont abundance data are relatively uniform among the three species and do not scale consistently with intra shell B/Ca, or with observed species-specific offsets in B/Ca or d11B. Implications of these findings for foraminiferal physiology and biomineralization processes are discussed. #--------------------------------------- # Funding_Agency # Funding_Agency_Name: National Science Foundation # Grant: OCE12-32987 #--------------------------------------- # Site_Information # Site_Name: Isla Magueyes, La Parguera and Santa Catalina Island # Location: Global Ocean # Northernmost_Latitude: 33.26 # Southernmost_Latitude: 17.92 # Easternmost_Longitude: -67 # Westernmost_Longitude: -118.29 # Elevation: #--------------------------------------- # Data_Collection # Collection_Name: ParagueraCatalina fluor and chlorophyll Hoenisch2020 # First_Year: 2015 # Last_Year: 2015 # Time_Unit: AD # Core_Length: # Notes: Laboratory Cultures #--------------------------------------- # Chronology_Information # Chronology: #--------------------------------------- # Variables # Data variables follow that are preceded by "##" in columns one and two. # Variables list, one per line, shortname-tab-longname components (9 components: what, material, error, units, seasonality, archive, detail, method, C or N for Character or Numeric data) ## analysis_date notes,,,,,paleoceanography,,,C,analysis date ## foram_id sample identification,,,,,paleoceanography,,,C,foram identification number ## largest_shell_dim diameter,foraminifer,,micrometer,,paleoceanography,,,N,largest shell dimension ## fluor_method notes,,,,,paleoceanography,,,C,fluorometer method applied for FRRF includes which method was applied first and second ## Fm-avg fluorescence,,,dimensionless,,paleoceanography,averaged,,N,fast repetition rate fluorometry; maximum flurorescence yield in darkness ## Fm-avg_err fluorescence,,one standard deviation,dimensionless,,paleoceanography,,,N,fast repetition rate fluorometry; error ## Fv/Fm-avg potential photochemical efficiency,,,dimensionless,,paleoceanography,averaged,,N,fast repetition rate fluorometry; Potential photochemical efficiency of open reaction centers ((Fm - F0)/Fm) ## FvFm-avg_err potential photochemical efficiency,,one standard deviation,dimensionless,,paleoceanography,,,N,fast repetition rate fluorometry; error ## Sig.D-avg effective absorption cross section,,,square angstrom per quanta,,paleoceanography,averaged,,N,fast repetition rate fluorometry;Effective absorption cross section of PSII in darkness ## Sig.D-avg_err effective absorption cross section,,one standard deviation,square angstrom per quanta,,paleoceanography,,,N,fast repetition rate fluorometry; error ## F0-avg fluorescence,,,dimensionless,,paleoceanography,averaged,,N,fast repetition rate fluorometry; Minimum fluorescence yield in darkness ## F0-avg_err fluorescence,,one standard deviation,dimensionless,,paleoceanography,,,N,fast repetition rate fluorometry; error ## Chlor_a chlorophyll,,,nanogram,,paleoceanography,,,N,chlorophyll a concentration per foraminiferan in nanogram/foraminifer; fluorometry ## notes notes,,,,,paleoceanography,,,C, #------------------------ # Data: # Data lines follow (have no #) # Data line format - tab-delimited text, variable short name as header # Missing_Values: NA analysis_date foram_id largest_shell_dim fluor_method Fm-avg Fm-avg_err Fv/Fm-avg FvFm-avg_err Sig.D-avg Sig.D-avg_err F0-avg F0-avg_err Chlor_a notes 4/15/2015 sacculifer 809 176 this study 0.08749 0.011459647 0.40850 0.0157741 622.39 63.12249314 0.0517 0.006253 NA NA 4/15/2015 sacculifer 809 176 Fujiki et al. 2014 0.15679 0.048566017 0.47661 0.0190193 601.35 62.20047929 0.0459 0.00609 NA NA 4/20/2015 sacculifer 1036 208 this study 0.04505 0.002890868 0.33110 0.0227449 504.00 14.12690879 0.0301 0.002179 0 NA 4/20/2015 sacculifer 1036 208 Fujiki et al. 2014 0.10681 0.015079291 0.39526 0.0303603 482.21 41.96474307 0.0368 0.010787 0 NA 4/24/2015 sacculifer 1305 253 this study 0.06818 0.00820951 0.40012 0.0109288 520.09 48.14603849 0.0409 0.005264 NA NA 4/24/2015 sacculifer 1305 253 Fujiki et al. 2014 0.20220 0.100386107 0.45953 0.0198043 510.29 56.27679211 0.0535 0.018094 NA NA 4/13/2015 sacculifer 806 254 this study 0.13123 0.013450183 0.40025 0.0100557 640.87 60.14735794 0.0787 0.007914 NA NA 4/13/2015 sacculifer 806 254 Fujiki et al. 2014 0.14992 0.021760482 0.46356 0.0105316 584.20 70.62760345 0.0770 0.008927 NA NA 4/10/2015 sacculifer 664 313 this study 0.12535 0.055828866 0.40622 0.0288136 490.12 47.34864447 0.0734 0.029239 1.57 NA 4/10/2015 sacculifer 664 313 Fujiki et al. 2014 0.16720 0.027335499 0.45690 0.0256517 462.88 43.23685975 0.0721 0.016182 1.57 NA 4/13/2015 sacculifer 758 383 this study 0.42529 0.015141819 0.42954 0.0120053 504.02 26.45316217 0.2426 0.011096 NA NA 4/13/2015 sacculifer 758 383 Fujiki et al. 2014 0.49866 0.053677588 0.49234 0.0019832 473.26 29.62812448 0.2393 0.008451 NA NA 4/10/2015 sacculifer 655 415 this study 0.78661 0.069403219 0.44694 0.0038465 574.53 44.6996102 0.4350 0.037627 7.19 NA 4/10/2015 sacculifer 655 415 Fujiki et al. 2014 0.83486 0.071987285 0.51146 0.0070722 552.18 42.10777241 0.3845 0.035326 7.19 NA 4/15/2015 sacculifer 841 491 this study 0.34050 0.058737768 0.43625 0.0055299 555.67 41.43083876 0.1920 0.033768 2.07 NA 4/15/2015 sacculifer 841 491 Fujiki et al. 2014 0.44959 0.057777616 0.49517 0.0067755 525.13 53.86331538 0.1831 0.02725 2.07 NA 4/22/2015 sacculifer 1156 523 this study 0.40754 0.149948732 0.43059 0.0128027 523.55 40.18159483 0.2315 0.083919 6.31 NA 4/22/2015 sacculifer 1156 523 Fujiki et al. 2014 0.39005 0.113490859 0.48602 0.0085462 490.61 37.31249104 0.1906 0.05264 6.31 NA 3/31/2015 sacculifer 418 544 this study 0.15878 0.034884232 0.36932 0.0092655 486.39 65.46246589 0.0999 0.020824 2.87 NA 4/22/2015 sacculifer 1161 558 this study 0.28459 0.125164354 0.29165 0.0130219 501.26 44.05266226 0.2030 0.093372 7.68 NA 4/22/2015 sacculifer 1161 558 Fujiki et al. 2014 0.31602 0.088173899 0.36667 0.0131931 444.93 59.25747753 0.1738 0.042055 7.68 NA 4/20/2015 sacculifer 1123 554 this study 0.27953 NA 0.34894 NA 636.97 NA NA NA 3.65 cuvette was not rotated during FRRF analysis, so there is no uncertainty estimate for this specimen 4/20/2015 sacculifer 1123 554 Fujiki et al. 2014 0.27490 0.018641009 0.42190 0.0108764 497.89 60.55684436 0.1439 0.007141 3.65 NA 4/22/2015 sacculifer 1204 637 this study 0.13165 0.029894364 0.39288 0.0128141 493.64 50.02579201 0.0796 0.016567 NA NA 4/22/2015 sacculifer 1204 637 Fujiki et al. 2014 0.28911 0.141382187 0.43445 0.0082393 455.60 45.74891342 0.0853 0.021859 NA NA 4/10/2015 ruber 716 186 this study 0.04207 0.006572494 0.32308 0.0394184 591.96 34.89510902 0.0285 0.005143 0 NA 4/10/2015 ruber 716 186 Fujiki et al. 2014 0.13098 0.055912843 0.38812 0.0370487 595.18 34.4308494 0.0414 0.016842 0 NA 4/10/2015 ruber 712 190 this study 0.14594 0.027052649 0.42378 0.0477173 517.62 72.22017446 0.0814 0.013687 1.38 NA 4/10/2015 ruber 712 190 Fujiki et al. 2014 0.19118 0.013543693 0.51044 0.0078013 517.09 36.07843629 0.0792 0.002385 1.38 NA 4/10/2015 ruber 656 231 this study 0.17049 0.012659442 0.44644 0.0146137 579.29 19.43633129 0.0943 0.006403 1.53 NA 4/10/2015 ruber 656 231 Fujiki et al. 2014 0.23648 0.061240769 0.49703 0.0120883 573.04 20.06619612 0.0859 0.006816 1.53 NA 4/10/2015 ruber 826 291 this study 0.19477 0.147169838 0.39643 0.0164679 528.71 60.76938712 0.1183 0.091265 NA NA 4/10/2015 ruber 826 291 Fujiki et al. 2014 0.17756 0.00791735 0.46520 0.0134587 480.21 62.84573934 0.0764 0.006818 NA NA 4/1/2015 ruber 391 265 this study 0.29172 0.010141676 0.41877 0.0055896 633.28 34.76326557 0.1696 0.005956 NA NA 4/1/2015 ruber 391 265 Fujiki et al. 2014 0.37541 0.036819275 0.47528 0.0046357 612.16 12.86701731 0.1680 0.019812 NA NA 4/10/2015 ruber 713 342 this study 0.26167 0.074616184 0.40425 0.033253 594.37 68.21842922 0.1575 0.04991 2.57 NA 4/10/2015 ruber 713 342 Fujiki et al. 2014 0.43376 0.09827658 0.46137 0.0060932 593.77 22.39724581 0.1690 0.006209 2.57 NA 4/8/2015 ruber 654, 648, 704 338, 350, 316 this study 0.10821 0.056371121 0.12927 0.0161014 516.87 30.50636333 0.1920 0.030463 NA pooled several specimens for FRRF analysis in the same cuvette, FRRF data possibly not comparable to single foraminifer analyses 4/8/2015 ruber 654, 648, 704 338, 350, 316 Fujiki et al. 2014 0.11799 0.039896181 0.14874 0.0146408 469.03 40.9391946 0.1814 0.02225 NA pooled several specimens for FRRF analysis in the same cuvette, FRRF data possibly not comparable to single foraminifer analyses 4/1/2015 ruber 449 338 this study 0.18661 0.023908243 0.38721 0.0140097 561.76 35.43295113 0.1145 0.016667 NA NA 4/1/2015 ruber 449 338 Fujiki et al. 2014 0.30761 0.082548584 0.44906 0.0135107 534.53 28.20607738 0.1338 0.028959 NA NA 4/1/2015 ruber 459 352 this study 0.36906 0.094973785 0.40641 0.0120489 533.25 91.64908648 0.2186 0.053696 NA NA 4/1/2015 ruber 459 352 Fujiki et al. 2014 0.43263 0.023490807 0.45638 0.0088906 500.63 82.03917252 0.2065 0.034248 NA NA 3/30/2015 ruber 336, 280, 263, 338 382, 379, 376, 390 this study 0.32538 0.035514277 0.09760 0.0054683 418.15 34.79899631 0.1982 0.020418 2.69 pooled several specimens for FRRF analysis in the same cuvette, FRRF data possibly not comparable to single foraminifer analyses 3/31/2015 ruber 364 425 this study 0.19302 0.031054964 0.38158 0.0139462 535.20 93.00906581 0.1194 0.019546 NA NA 3/31/2015 ruber 350, 356, 290 412, 426, 405 this study 0.16665 0.099459651 0.14487 0.0066523 458.33 44.20146101 0.2824 0.054934 8.41 pooled several specimens for FRRF analysis in the same cuvette, FRRF data possibly not comparable to single foraminifer analyses 4/1/2015 ruber 396 454 this study 0.29304 0.022010771 0.43326 0.0089312 551.69 54.71574095 0.1661 0.012407 NA NA 4/1/2015 ruber 396 454 Fujiki et al. 2014 0.52686 0.292215929 0.48593 0.0063608 531.26 59.05413915 0.2297 0.162104 NA NA 4/20/2015 ruber 1043 479 this study 0.30632 0.101770729 0.33890 0.007138 471.39 64.50494715 0.2023 0.066873 6.49 NA 4/20/2015 ruber 1043 479 Fujiki et al. 2014 0.35560 0.094036803 0.41158 0.0025831 421.48 72.76586015 0.1867 0.05773 6.49 NA 3/31/2015 ruber 419, 407 463, 479 this study 0.22057 0.029448217 0.20721 0.004446 555.60 34.11384035 0.2584 0.018705 3.06 pooled several specimens for FRRF analysis in the same cuvette, FRRF data possibly not comparable to single foraminifer analyses 3/31/2015 ruber 361 480 this study 0.09220 0.029358041 0.30923 0.0152789 506.82 90.46458075 0.0637 0.020225 3.06 specimens pooled with row above for pigment analysis, but FRRF analysis was done individually 3/31/2015 ruber 294, 383 525, 528 this study 0.12188 0.057872817 0.20891 0.0049061 551.04 52.46210131 0.3393 0.031655 4.89 pooled several specimens for FRRF analysis in the same cuvette, FRRF data possibly not comparable to single foraminifer analyses 3/30/2015 ruber 278, 273, 274 501, 510, 545 this study 0.15573 0.092163145 0.12886 0.0113498 453.17 27.44962416 0.2868 0.058622 10.46 pooled several specimens for FRRF analysis in the same cuvette, FRRF data possibly not comparable to single foraminifer analyses 4/22/2015 ruber 1176 634 this study 0.12979 0.028506153 0.36078 0.0202449 392.83 94.13632077 0.0826 0.015949 8.13 NA 4/22/2015 ruber 1176 634 Fujiki et al. 2014 0.21781 0.076717944 0.41727 0.0145234 347.17 92.71805548 0.0659 0.003161 8.13 NA 4/1/2015 universa 206 462 this study 0.20324 0.0124019 0.39635 0.0084278 495.30 39.98840772 0.1227 0.008643 3.25 NA 4/1/2015 universa 194 471 this study 0.18850 0.0320031 0.37553 0.0099435 544.59 61.88354137 0.1272 0.0197 2.56 NA 4/22/2015 universa 1155 496 this study 0.39962 0.062302317 0.40817 0.0011822 617.31 47.13434177 0.2364 0.036587 3.48 NA 4/22/2015 universa 1155 496 Fujiki et al. 2014 0.47415 0.039934336 0.47094 0.003862 566.71 32.59461712 0.2300 0.006154 3.48 NA 3/25/2015 universa 60 478 this study 0.38747 0.058690051 0.45995 0.0065155 463.87 70.01975666 0.2093 0.032582 NA NA 3/25/2015 universa 58, 56, 48, 60 472, 500, 492, 478 this study NA NA NA NA NA NA NA NA 4.85 pooled specimens for pigment analysis 4/1/2015 universa 295 480 this study 0.21054 0.1211959 0.41370 0.0136848 515.89 58.26801457 0.1223 0.066924 1.82 NA 3/27/2015 universa 205, 91, 197 521, 527, 508 this study 0.77090 0.160810619 0.10230 0.0176969 505.89 39.19077986 0.5329 0.101395 3.73 pooled several specimens for FRRF analysis in the same cuvette, FRRF data possibly not comparable to single foraminifer analyses 3/27/2015 universa 180 503 this study 0.19620 0.160810619 0.29939 0.0176969 410.76 39.19077986 0.1375 0.101395 3.73 specimens pooled with row above for pigment analysis, but FRRF analysis was done individually 3/25/2015 universa 68 520 this study 0.55780 0.091843973 0.43726 0.0045541 512.32 58.60891662 0.3137 0.05043 NA NA 3/25/2015 universa 68, 178, 49, 55 520, 506, 516, 514 this study NA NA NA NA NA NA NA NA 5.80 pooled specimens for pigment analysis 4/22/2015 universa 1140 568 this study 0.12354 0.034441514 0.40009 0.0181488 405.23 88.68505179 0.0737 0.018992 2.24 NA 4/22/2015 universa 1140 568 Fujiki et al. 2014 0.20225 0.041496337 0.47667 0.0190712 391.93 95.91080487 0.0832 0.026836 2.24 NA 3/25/2015 universa 70 570 this study 0.73809 0.117978036 0.41785 0.0074949 556.08 37.91387174 0.4295 0.067395 NA NA 3/25/2015 universa 70, 69, 165 570, 553, 552 this study NA NA NA NA NA NA NA NA 9.55 pooled specimens for pigment analysis 4/15/2015 universa 914 555 this study 0.62119 0.117441692 0.42149 0.0090378 574.43 64.70299896 0.3595 0.069478 5.55 NA 4/15/2015 universa 914 555 Fujiki et al. 2014 0.67544 0.057952873 0.49062 0.0099026 542.55 55.6736399 0.3272 0.032623 5.55 NA 3/30/2015 universa 251, 255, 326 575, 573, 559 NA NA NA NA NA NA NA NA NA 22.71 NA 4/22/2015 universa 1160 609 this study 0.77067 0.110168474 0.41528 0.0139965 586.24 43.47017351 0.4516 0.074557 13.37 NA 4/22/2015 universa 1160 609 Fujiki et al. 2014 0.88209 0.156576653 0.49082 0.0061574 529.31 33.51271768 0.4393 0.084544 13.37 NA 4/15/2015 universa 799 620 this study 1.70242 0.446600395 0.42905 0.023106 526.11 167.088924 0.9805 0.27621 6.45 NA 4/15/2015 universa 799 620 Fujiki et al. 2014 1.88958 0.071170585 0.47018 0.017764 560.13 27.4556105 0.9328 0.03773 6.45 NA 4/15/2015 universa 859 678 this study 0.83402 0.189727515 0.44570 0.005541 492.31 26.9220286 0.4622 0.10491 20.37 NA 4/15/2015 universa 859 678 Fujiki et al. 2014 0.88264 0.167335404 0.49755 0.00322 464.72 24.9158112 0.4291 0.09578 20.37 NA 4/22/2015 universa 1153 676 this study 1.27805 0.063343166 0.45494 0.010992 473.20 39.3123554 0.6965 0.03453 19.92 NA 4/22/2015 universa 1153 676 Fujiki et al. 2014 1.31921 0.113581559 0.50854 0.0020018 457.60 31.99813809 0.6346 0.051871 19.92 NA 4/10/2015 universa 734 675 this study 0.99057 0.210419096 0.41256 0.0043819 536.03 26.50247361 0.5825 0.127961 22.55 NA 4/10/2015 universa 734 675 Fujiki et al. 2014 0.97454 0.051026862 0.48116 0.0018607 505.15 31.22298296 0.4900 0.03645 22.55 NA 4/10/2015 universa 671 670 this study 1.58986 0.232038468 0.41052 0.0090003 562.58 28.25395731 0.9385 0.149363 21.49 NA 4/10/2015 universa 671 670 Fujiki et al. 2014 1.49555 0.227176873 0.47976 0.0037758 504.83 41.73329962 0.7685 0.109892 21.49 NA 4/15/2015 universa 906 707 this study 0.81157 0.120027722 0.44885 0.0229586 466.37 41.32075418 0.4487 0.081987 13.74 NA 4/15/2015 universa 906 707 Fujiki et al. 2014 0.83457 0.14561798 0.51227 0.0102474 439.87 42.39093134 0.4057 0.07575 13.74 NA