# Monterey Bay Bamboo Coral phenylalanine d15N Data from 1977-2004 CE #---------------------------------------------------- # World Data Service for Paleoclimatology, Boulder # and # NOAA Paleoclimatology Program # National Centers for Environmental Information (NCEI) #---------------------------------------------------- # Template Version 4.0 # Encoding: UTF-8 # NOTE: Please cite original publication, NOAA Landing Page URL, dataset and publication DOIs (where available), and date accessed when using downloaded data. If there is no publication information, please cite investigator, study title, NOAA Landing Page URL, and date accessed. # # Description/Documentation lines begin with # # Data lines have no # # # NOAA_Landing_Page: https://www.ncei.noaa.gov/access/paleo-search/study/22230 # Landing_Page_Description: NOAA Landing Page of this file's parent study, which includes all study metadata. # # Study_Level_JSON_Metadata: https://www.ncei.noaa.gov/pub/data/metadata/published/paleo/json/noaa-coral-22230.json # Study_Level_JSON_Description: JSON metadata of this data file's parent study, which includes all study metadata. # # Data_Type: Corals and Sclerosponges # # Dataset_DOI: 10.25921/7v2t-gr53 # # Science_Keywords: #-------------------- # Resource_Links # # Data_Download_Resource: https://www.ncei.noaa.gov/pub/data/paleo/coral/east_pacific/broek2013/broek2013-noaa.txt # Data_Download_Description: NOAA Template File; d15N Data # #-------------------- # Contribution_Date # Date: 2017-06-06 #-------------------- # File_Last_Modified_Date # Date: 2025-08-27 #-------------------- # Title # Study_Name: Monterey Bay Bamboo Coral phenylalanine d15N Data from 1977-2004 CE #-------------------- # Investigators # Investigators: Broek, T.A.B.; Walker, B.D.; Andreasen, D.H.; McCarthy, M.D. #-------------------- # Description_Notes_and_Keywords # Description: Measurement precision- bulk d15N: <0.1 per mil AIR; d15N_Phe (GC-C-IRMS): 0.6 per mil AIR; d15N_Phe (HPLC-EA-IRMS): < 0.3 per mil AIR #-------------------- # Publication # Authors: Broek, T.A.B., B.D. Walker, D.H. Andreasen, and M.D. McCarthy # Journal_Name: Rapid Communications in Mass Spectrometry # Published_Title: High-precision measurement of phenylalanine d15N values for environmental samples: A new approach coupling high-pressure liquid chromatography purification and elemental analyzerisotope ratio mass spectrometry # Published_Date_or_Year: 2013 # Volume: 27 # Issue: # Pages: 2327-2337 # Report_Number: # DOI: 10.1002/rcm.6695 # Full_Citation: # Abstract: RATIONALE: Compound-specific isotope analysis of individual amino acids (CSI-AA) is a powerful new tool fortracing nitrogen (N) source and transformation in biogeochemical cycles. Specifically, the d15N value of phenylalanine (d15NPhe) represents an increasingly used proxy for source d15N signatures, with particular promise for paleoceanographic applications. However, current derivatization/gas chromatography methods require expensive and relatively uncommon instrumentation, and have relatively low precision, making many potential applications impractical. METHODS: A new offline approach has been developed for high-precision d15N measurements of amino acids (d15NAA), optimized for d15NPhe values. Amino acids (AAs) are first purified via high-pressure liquid chromatography (HPLC), using a mixed-phase column and automated fraction collection. The d15N values are determined via offline elemental analyzer-isotope ratio mass spectrometry (EA-IRMS). RESULTS: The combinedHPLC/EA-IRMSmethod separated most proteinAAs with sufficient resolution to obtain accurate d15N values, despite significant intra-peak isotopic fractionation. For d15NPhe values, the precision was ±0.16‰ for standards, 4Å~ better than gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS; ±0.64‰). We also compared a d15NPhe paleo-record from a deep-sea bamboo coral from Monterey Bay, CA, USA, using our method versus GC/C/IRMS. The two methods produced equivalent d15NPhe values within error; however, the d15NPhe values from HPLC/EA-IRMS had approximately twice the precision of GC/C/IRMS (average stdev of 0.27‰±0.14‰ vs 0.60‰±0.20‰, respectively). CONCLUSIONS: These results demonstrate that offline HPLC represents a viable alternative to traditional GC/C/IMRS for d15NAA measurement. HPLC/EA-IRMS is more precise and widely available, and therefore useful in applications requiring increased precision for data interpretation (e.g. d15N paleoproxies). #-------------------- # Funding_Agency # Funding_Agency_Name: # Grant: #-------------------- # Site_Information # Site_Name: T1104 A7 Monterey Canyon # Location: North Pacific Ocean # Northernmost_Latitude: 36.74423 # Southernmost_Latitude: 36.74423 # Easternmost_Longitude: -122.037215 # Westernmost_Longitude: -122.037215 # Elevation_m: -870 #-------------------- # Data_Collection # Collection_Name: Monterey Bay T1104 A7 Broek2013 # First_Year: 1977 # Last_Year: 2004 # Time_Unit: CE # Core_Length_m: # Parameter_Keywords: chemistry # Notes: #-------------------- # Chronology_Information # Chronology: Radiocarbon # Chronology_Download_Resource: https://www.ncei.noaa.gov/pub/data/paleo/templates/noaa-wds-paleo-14c-terms.csv # Chronology_Download_Description: Radiocarbon terms and definitions. # Chronology_Notes: Lab codes are CAMS numbers. d13C values are the assumed values according to Stuiver and Polach (Radiocarbon, v. 19, p.355, 1977) when given without decimal places. Values measured for the material itself are given with a single decimal place. The quoted age is in radiocarbon years using the Libby half life of 5568 years and following the conventions of Stuiver and Polach (ibid.). Radiocarbon concentration is given as fraction Modern, D14C, and conventional radiocarbon age. Sample preparation backgrounds have been subtracted, based on measurements of samples of 14C-free coal. Backgrounds were scaled relative to sample size. # Rejection_Rationale: # Reservoir_Method: # Calibration_Method: # Age_Model_Method: # Missing_Values: # Chronology_Table: # lab_code samp_id notes d13C_PDB frac_mod frac_mod_uncert Delta_14C_permil Delta_14C_uncert_permil age_14C_BP1950 age_14C_uncert_yr core_id depth_mm age_calib_CE # 157923 A7-1 N101060 -16 1.01546008424921 3.66175340989682E-03 15.4600842492096 3.66175340989682 NaN NaN T1104_A7 0 2007 # 157924 A7-3 N101061 -16 1.03184077070434 3.38819464016935E-03 31.8407707043411 3.38819464016935 NaN NaN T1104_A7 1.4 1996 # 157925 A7-5 N101062 -16 1.03557723414341 3.60429993968773E-03 35.5772341434093 3.60429993968773 NaN NaN T1104_A7 2 1992 # 157926 A7-9 N101063 -16 1.0376736662578 3.56697252370979E-03 37.6736662578006 3.56697252370979 NaN NaN T1104_A7 3.1 1983 # 157927 A7-11 N101064 -16 1.05255056662969 3.3047681224007E-03 52.5505666296895 3.3047681224007 NaN NaN T1104_A7 3.6 1980 # 157928 A7-17 N101065 -16 0.985944608832484 3.23732807153568E-03 -14.0553911675162 3.23732807153568 115 30 T1104_A7 5.8 1962 # 157929 A7-21 N101066 -16 0.923126621821718 3.03287939743147E-03 -76.8733781782818 3.03287939743147 645 30 T1104_A7 6.7 1955 # 157930 A7-39 N101067 -16 0.902049109128138 2.96564871268418E-03 -97.950890871862 2.96564871268418 830 30 T1104_A7 9.6 1933 #-------------------- # Variables # PaST_Thesaurus_Download_Resource: https://www.ncei.noaa.gov/access/paleo-search/skos/past-thesaurus.rdf # PaST_Thesaurus_Download_Description: Paleoenvironmental Standard Terms (PaST) Thesaurus terms, definitions, and relationships in SKOS format. # # Data variables follow that are preceded by '##' in columns one and two. # Variables list, one per line, shortname-tab-var components: what, material, error, units, seasonality, data type, detail, method, C or N for Character or Numeric data) # ## samp_num sample identification,,,,,corals and sclerosponges,,,N, ## distance depth,,,millimeter,,corals and sclerosponges,,,N,mean distance from outer margin of section ## dist_end depth at sample end,,,millimeter,,corals and sclerosponges,,,N, ## dist_start depth at sample start,,,millimeter,,corals and sclerosponges,,,N, ## Date age,,,year Common Era,,corals and sclerosponges,,,N,14C age model mean ## d15N-bulk delta 15N,Isidella sp.,,per mil AIR,,corals and sclerosponges,raw,elemental analysis;isotope ratio mass spectrometry,N, ## d15N_Phe_GC-C-IRMS delta 15N,Isidella sp.;phenylalanine,,per mil AIR,,corals and sclerosponges,raw,gas chromatography;isotope ratio mass spectrometry,N,Used GC-Combustion-IRMS ## d15N_Phe_HPLC-EA-IRMS delta 15N,Isidella sp.;phenylalanine,,per mil AIR,,corals and sclerosponges,raw,elemental analysis;isotope ratio mass spectrometry,N,first purified via high-pressure liquid chromatography #-------------------- # Data: # Data lines follow (have no #) # Data line format - tab-delimited text, variable short name as header # Missing_Values: samp_num distance dist_end dist_start Date d15N-bulk d15N_Phe_GC-C-IRMS d15N_Phe_HPLC-EA-IRMS 1 0.66 0.34 0.98 2004.5 15.8 10.14 9.8 2 1.9 1.695 2.105 2000.5 15.02 9.82 9.87 3 2.1 1.84 2.36 1997 14.84 9.51 9.03 4 2.89 2.62 3.16 1993 14.36 10.25 9.82 5 3.86 3.475 4.245 1988 14.49 9.83 9.19 6 4.8 4.555 5.045 1983.5 15.26 10.4 9.72 7 6.2 5.545 6.855 1977 15.29 11.58 10.99