# Gulf of Alaska, Hawai'ian Islands, Central California Coast Amino Acid d13C and d15N Data #----------------------------------------------------------------------- # World Data Service for Paleoclimatology, Boulder # and # NOAA Paleoclimatology Program #----------------------------------------------------------------------- # Template Version 4.0 # Encoding: UTF-8 # NOTE: Please cite original publication, NOAA Landing Page URL, dataset and publication DOIs (where available), and date accessed when using downloaded data. # If there is no publication information, please cite investigator, study title, NOAA Landing Page URL, and date accessed. # # Description/Documentation lines begin with '#' followed by a space # Data lines have no '#' # # NOAA_Landing_Page: https://www.ncei.noaa.gov/access/paleo-search/study/37139 # Landing_Page_Description: NOAA Landing Page of this file's parent study, which includes all study metadata. # # Study_Level_JSON_Metadata: https://www.ncei.noaa.gov/pub/data/metadata/published/paleo/json/noaa-coral-37139.json # Study_Level_JSON_Description: JSON metadata of this data file's parent study, which includes all study metadata. # # Data_Type: Corals and Sclerosponges # # Dataset_DOI: 10.25921/1xnj-ez49 # # Science_Keywords: #--------------------------------------- # Resource_Links # # Data_Download_Resource: https://www.ncei.noaa.gov/pub/data/paleo/coral/north_pacific/mcmahon2018/mcmahon2018-aa.txt # Data_Download_Description: NOAA Template File; North Pacific d13C, d15N Amino Acid Data # #--------------------------------------- # Contribution_Date # Date: 2022-12-08 #--------------------------------------- # File_Last_Modified_Date # Date: 2025-05-05 #--------------------------------------- # Title # Study_Name: Gulf of Alaska, Hawai'ian Islands, Central California Coast Amino Acid d13C and d15N Data #--------------------------------------- # Investigators # Investigators: McMahon, Kelton W.; Williams, Branwen; Guilderson, T.P.; Glynn, D.S.; McCarthy, M.D. #--------------------------------------- # Description_Notes_and_Keywords # Description: Species used: Primnoa pacifica, Isidella spp, Kulamanamana haumeaae. Samples collected between -20 and -1250m. Primnoas collected live 2010, 2013; Isidella spp collected 2014; Kulamanamana collected 2004 and 2007. Samples are all live-collected proteinaceous deep-sea corals. Live polyps are assumed to live 1-3 years. Outermost skeletal material from the coral's skeleton is assumed to be contemporaneous with the polyps. #--------------------------------------- # Publication # Authors: McMahon, K.W., Williams, B., Guilderson, T.P., Glynn, D.S., McCarthy, M.D. # Published_Date_or_Year: 2018 # Published_Title: Calibrating amino acid d13C and d15N offsets between polyp and protein skeleton to develop proteinaceous deep-sea corals as paleoceanographic archives # Journal_Name: Geochimica et Cosmochimica Acta # Volume: 220 # Edition: # Issue: # Pages: 261-275 # Report_Number: # DOI: 10.1016/j.gca.2017.09.048 # Online_Resource: # Full_Citation: # Abstract: Compound-specific stable isotopes of amino acids (CSI-AA) from proteinaceous deep-sea coral skeletons have the potential to improve paleoreconstructions of plankton community composition, and our understanding of the trophic dynamics and biogeochemical cycling of sinking organic matter in the Ocean. However, the assumption that the molecular isotopic values preserved in protein skeletal material reflect those of the living coral polyps has never been directly investigated in proteinaceous deep-sea corals. We examined CSI-AA from three genera of proteinaceous deep-sea corals from three oceanographically distinct regions of the North Pacific: Primnoa from the Gulf of Alaska, Isidella from the Central California Margin, and Kulamanamana from the North Pacific Subtropical Gyre. We found minimal offsets in the d13C values of both essential and non-essential AAs, and in the d15N values of source AAs, between paired samples of polyp tissue and protein skeleton. Using an essential AA d13C fingerprinting approach, we show that estimates of the relative contribution of eukaryotic microalgae and prokaryotic cyanobacteria to the sinking organic matter supporting deep-sea corals are the same when calculated from polyp tissue or recently deposited skeletal tissue. The d15N values of trophic AAs in skeletal tissue, on the other hand, were consistently 3–4‰ lower than polyp tissue for all three genera. We hypothesize that this offset reflects a partitioning of nitrogen flux through isotopic branch points in the synthesis of polyp (fast turnover tissue) and skeleton (slow, unidirectional incorporation). This offset indicates an underestimation, albeit correctable, of approximately half a trophic position from gorgonin protein-based deep-sea coral skeleton. Together, our observations open the door for applying many of the rapidly evolving CSI-AA based tools developed for metabolically active tissues in modern systems to archival coral tissues in a paleoceanographic context. #--------------------------------------- # Funding_Agency # Funding_Agency_Name: US National Science Foundation # Grant: OCE 1061689, OCE 1635527 #--------------------------------------- # Funding_Agency # Funding_Agency_Name: NOAA/National Undersea Research Program # Grant: NA08OAR4300817 #--------------------------------------- # Site_Information # Site_Name: Gulf of Alaska, Hawai'ian Islands, Central California Coast # Location: North Pacific Ocean # Northernmost_Latitude: 59.5 # Southernmost_Latitude: 18.7 # Easternmost_Longitude: -122.3 # Westernmost_Longitude: -165.4 # Elevation_m: #--------------------------------------- # Data_Collection # Collection_Name: North Pacific d13C, d15N McMahon2018 # First_Year: # Last_Year: # Time_Unit: # Core_Length_m: # Parameter_Keywords: carbon isotopes, chemistry # Notes: #--------------------------------------- # Chronology_Information # Chronology: # # Samples are all live-collected proteinaceous deep-sea corals. Live polyps are assumed to live 1-3 years. # Outermost skeletal material from the coral's skeleton is assumed to be contemporaneous with the polyps. # #--------------------------------------- # Variables # PaST_Thesaurus_Download_Resource: https://www.ncei.noaa.gov/access/paleo-search/skos/past-thesaurus.rdf # PaST_Thesaurus_Download_Description: Paleoenvironmental Standard Terms (PaST) Thesaurus terms, definitions, and relationships in SKOS format. # # Data variables follow that are preceded by "##" in columns one and two. # Variables list, one per line, shortname-tab-var components: what, material, error, units, seasonality, data type, detail, method, C or N for Character or Numeric data) # ## Taxa sample identification,,,,,corals and sclerosponges,,,C,Species ID ## ID sample identification,,,,,corals and sclerosponges,,,C,Sample ID ## Region notes,,,,,corals and sclerosponges,,,C,Geographic Area ## Depth collection water depth,,,meter,,corals and sclerosponges,,,N,Water depth of sample (mbsl) ## Lat_N latitude,,,degree north,,corals and sclerosponges,,,N, ## Long_W longitude,,,degree west,,corals and sclerosponges,,,N, ## Skel_d13C delta 13C,coral,,per mil VPDB,,corals and sclerosponges,,isotope ratio mass spectrometry,N,d13C of outermost skeleton; measurement precision: 0.1 ## Polyp_d13C_lipids delta 13C,coral,,per mil VPDB,,corals and sclerosponges,,isotope ratio mass spectrometry,N,d13C of polyps without removing lipid material; measurement precision: 0.1 ## Polyp_w/o_lipids delta 13C,coral,,per mil VPDB,,corals and sclerosponges,,isotope ratio mass spectrometry,N,d13C of polyps after lipid extraction; measurement precision: 0.1 ## Skel_d15N delta 15N,coral,,per mil AIR,,corals and sclerosponges,,isotope ratio mass spectrometry,N,d15N of outermost skeleton; measurement precision: 0.1 ## Polyp_lipids_d15N delta 15N,coral,,per mil AIR,,corals and sclerosponges,,isotope ratio mass spectrometry,N,d15N of polyps without lipid extraction; measurement precision: 0.1 ## Skel_C/N carbon/nitrogen,coral,,dimensionless,,corals and sclerosponges,,isotope ratio mass spectrometry,N,C:N atomic ratio of outermost skeletal material ## Polyp_lipids_C/N carbon/nitrogen,coral,,dimensionless,,corals and sclerosponges,,isotope ratio mass spectrometry,N,C:N atomic ratio of polyps without lipid extraction ## Polyp_w/o_lipids_C/N carbon/nitrogen,coral,,dimensionless,,corals and sclerosponges,,isotope ratio mass spectrometry,N,C:N atomic ratio of polyps with lipid extraction ## Ala_P_d13C delta 13C,alanine,,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp ## Ala_P_d13C_1s delta 13C,alanine,one standard deviation,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,uncertainty of <1 reflects one sigma sd of three replicate injections ## Asp_P_d13C delta 13C,aspartic acid and asparagine,,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp ## Asp_P_d13C_1s delta 13C,aspartic acid and asparagine,one standard deviation,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp; uncertainty of <1 reflects one sigma sd of three replicate injections ## Glu_P_d13C delta 13C,glutamic acid and glutamine,,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp ## Glu_P_d13C_1s delta 13C,glutamic acid and glutamine,one standard deviation,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp; uncertainty of <1 reflects one sigma sd of three replicate injections ## Gly_P_d13C delta 13C,Glycine,,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp ## Gly_P_d13C_1s delta 13C,Glycine,one standard deviation,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp; uncertainty of <1 reflects one sigma sd of three replicate injections ## Ile_P_d13C delta 13C,Isoleucine,,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp ## Ile_P_d13C_1s delta 13C,Isoleucine,one standard deviation,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp; uncertainty of <1 reflects one sigma sd of three replicate injections ## Leu_P_d13C delta 13C,Leucine,,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp ## Leu_P_d13C_1s delta 13C,Leucine,one standard deviation,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp; uncertainty of <1 reflects one sigma sd of three replicate injections ## Phe_P_d13C delta 13C,Phenylalanine,,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp ## Phe_P_d13C_1s delta 13C,Phenylalanine,one standard deviation,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp; uncertainty of <1 reflects one sigma sd of three replicate injections ## Pro_P_d13C delta 13C,Proline,,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp ## Pro_P_d13C_1s delta 13C,Proline,one standard deviation,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp; uncertainty of <1 reflects one sigma sd of three replicate injections ## Ser_P_d13C delta 13C,Serine,,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp ## Ser_P_d13C_1s delta 13C,Serine,one standard deviation,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp; uncertainty of <1 reflects one sigma sd of three replicate injections ## Thr_P_d13C delta 13C,Threonine,,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp ## Thr_P_d13C_1s delta 13C,Threonine,one standard deviation,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp; uncertainty of <1 reflects one sigma sd of three replicate injections ## Val_P_d13C delta 13C,Valine,,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp ## Val_P_d13C_1s delta 13C,Valine,one standard deviation,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp; uncertainty of <1 reflects one sigma sd of three replicate injections ## Ala_d15N delta 15N,Alanine,,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp ## Ala_d15N_1s delta 15N,Alanine,one standard deviation,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp; uncertainty of <1 reflects one sigma sd of three replicate injections ## Asp_P_d15N delta 15N,aspartic acid and asparagine,,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp ## Asp_P_d15N_1s delta 15N,aspartic acid and asparagine,one standard deviation,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp; uncertainty of <1 reflects one sigma sd of three replicate injections ## Glu_P_d15N delta 15N,glutamic acid and glutamine,,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp ## Glu_P_d15N_1s delta 15N,glutamic acid and glutamine,one standard deviation,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp; uncertainty of <1 reflects one sigma sd of three replicate injections ## Gly_P_d15N delta 15N,Glycine,,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp ## Gly_P_d15N_1s delta 15N,Glycine,one standard deviation,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp; uncertainty of <1 reflects one sigma sd of three replicate injections ## Ile_P_d15N delta 15N,Isoleucine,,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp ## Ile_P_d15N_1s delta 15N,Isoleucine,one standard deviation,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp; uncertainty of <1 reflects one sigma sd of three replicate injections ## Leu_P_d15N delta 15N,Leucine,,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp ## Leu_P_d15N_1s delta 15N,Leucine,one standard deviation,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp; uncertainty of <1 reflects one sigma sd of three replicate injections ## Lys_P_d15N delta 15N,Lysine,,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp ## Lys_P_d15N_1s delta 15N,Lysine,one standard deviation,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp; uncertainty of <1 reflects one sigma sd of three replicate injections ## Met_P_d15N delta 15N,Methionine,,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp ## Met_P_d15N_1s delta 15N,Methionine,one standard deviation,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp; uncertainty of <1 reflects one sigma sd of three replicate injections ## Phe_P_d15N delta 15N,Phenylalanine,,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp ## Phe_P_d15N_1s delta 15N,Phenylalanine,one standard deviation,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp; uncertainty of <1 reflects one sigma sd of three replicate injections ## Pro_P_d15N delta 15N,Proline,,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp ## Pro_P_d15N_1s delta 15N,Proline,one standard deviation,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp; uncertainty of <1 reflects one sigma sd of three replicate injections ## Ser_P_d15N delta 15N,Serine,,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp ## Ser_P_d15N_1s delta 15N,Serine,one standard deviation,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp; uncertainty of <1 reflects one sigma sd of three replicate injections ## Thr_P_d15N delta 15N,Threonine,,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp ## Thr_P_d15N_1s delta 15N,Threonine,one standard deviation,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp; uncertainty of <1 reflects one sigma sd of three replicate injections ## Val_P_d15N delta 15N,Valine,,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp ## Val_P_d15N_1s delta 15N,Valine,one standard deviation,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Polyp; uncertainty of <1 reflects one sigma sd of three replicate injections ## Ala_Skel_d13C delta 13C,Alanine,,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton ## Ala_Skel_d13C_1s delta 13C,Alanine,one standard deviation,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton; uncertainty of <1 reflects one sigma sd of three replicate injections ## Asp_Skel_d13C delta 13C,aspartic acid and asparagine,,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton ## Asp_Skel_d13C_1s delta 13C,aspartic acid and asparagine,one standard deviation,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton; uncertainty of <1 reflects one sigma sd of three replicate injections ## Glu_Skel_d13C delta 13C,glutamic acid and glutamine,,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton ## Glu_Skel_d13C_1s delta 13C,glutamic acid and glutamine,one standard deviation,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton; uncertainty of <1 reflects one sigma sd of three replicate injections ## Gly_Skel_d13C delta 13C,Glycine,,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton ## Gly_Skel_d13C_1s delta 13C,Glycine,one standard deviation,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton; uncertainty of <1 reflects one sigma sd of three replicate injections ## Ile_Skel_d13C delta 13C,Isoleucine,,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton ## Ile_Skel_d13C_1s delta 13C,Isoleucine,one standard deviation,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton; uncertainty of <1 reflects one sigma sd of three replicate injections ## Leu_Skel_d13C delta 13C,Leucine,,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton ## Leu_Skel_d13C_1s delta 13C,Leucine,one standard deviation,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton; uncertainty of <1 reflects one sigma sd of three replicate injections ## Phe_Skel_d13C delta 13C,Phenylalanine,,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton ## Phe_Skel_d13C_1s delta 13C,Phenylalanine,one standard deviation,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton; uncertainty of <1 reflects one sigma sd of three replicate injections ## Pro_Skel_d13C delta 13C,Proline,,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton ## Pro_Skel_d13C_1s delta 13C,Proline,one standard deviation,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton; uncertainty of <1 reflects one sigma sd of three replicate injections ## Ser_Skel_d13C delta 13C,Serine,,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton ## Ser_Skel_d13C_1s delta 13C,Serine,one standard deviation,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton; uncertainty of <1 reflects one sigma sd of three replicate injections ## Thr_Skel_d13C delta 13C,Threonine,,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton ## Thr_Skel_d13C_1s delta 13C,Threonine,one standard deviation,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton; uncertainty of <1 reflects one sigma sd of three replicate injections ## Val_Skel_d13C delta 13C,Valine,,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton ## Val_Skel_d13C_1s delta 13C,Valine,one standard deviation,per mil VPDB,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton; uncertainty of <1 reflects one sigma sd of three replicate injections ## Ala_Skel_d15N delta 15N,Alanine,,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton ## Ala_Skel_d15N_1s delta 15N,Alanine,one standard deviation,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton; uncertainty of <1 reflects one sigma sd of three replicate injections ## Asp_Skel_d15N delta 15N,aspartic acid and asparagine,,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton ## Asp_Skel_d15N_1s delta 15N,aspartic acid and asparagine,one standard deviation,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton; uncertainty of <1 reflects one sigma sd of three replicate injections ## Glu_Skel_d15N delta 15N,glutamic acid and glutamine,,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton ## Glu_Skel_d15N_1s delta 15N,glutamic acid and glutamine,one standard deviation,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton; uncertainty of <1 reflects one sigma sd of three replicate injections ## Gly_Skel_d15N delta 15N,Glycine,,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton ## Gly_Skel_d15N_1s delta 15N,Glycine,one standard deviation,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton; uncertainty of <1 reflects one sigma sd of three replicate injections ## Ile_Skel_d15N delta 15N,Isoleucine,,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton ## Ile_Skel_d15N_1s delta 15N,Isoleucine,one standard deviation,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton; uncertainty of <1 reflects one sigma sd of three replicate injections ## Leu_Skel_d15N delta 15N,Leucine,,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton ## Leu_Skel_d15N_1s delta 15N,Leucine,one standard deviation,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton; uncertainty of <1 reflects one sigma sd of three replicate injections ## Lys_Skel_d15N delta 15N,Lysine,,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton ## Lys_Skel_d15N_1s delta 15N,Lysine,one standard deviation,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton; uncertainty of <1 reflects one sigma sd of three replicate injections ## Met_Skel_d15N delta 15N,Methionine,,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton ## Met_Skel_d15N_1s delta 15N,Methionine,one standard deviation,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton; uncertainty of <1 reflects one sigma sd of three replicate injections ## Phe_Skel_d15N delta 15N,Phenylalanine,,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton ## Phe_Skel_d15N_1s delta 15N,Phenylalanine,one standard deviation,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton; uncertainty of <1 reflects one sigma sd of three replicate injections ## Pro_Skel_d15N delta 15N,Proline,,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton ## Pro_Skel_d15N_1s delta 15N,Proline,one standard deviation,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton; uncertainty of <1 reflects one sigma sd of three replicate injections ## Ser_Skel_d15N delta 15N,Serine,,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton ## Ser_Skel_d15N_1s delta 15N,Serine,one standard deviation,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton; uncertainty of <1 reflects one sigma sd of three replicate injections ## Thr_Skel_d15N delta 15N,Threonine,,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton ## Thr_Skel_d15N_1s delta 15N,Threonine,one standard deviation,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton; uncertainty of <1 reflects one sigma sd of three replicate injections ## Val_Skel_d15N delta 15N,Valine,,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton ## Val_Skel_d15N_1s delta 15N,Valine,one standard deviation,per mil AIR,,corals and sclerosponges,,gas chromatography; isotope ratio mass spectrometry,N,Outermost skeleton; uncertainty of <1 reflects one sigma sd of three replicate injections #------------------------ # Data: # Data lines follow (have no #) # Data line format - tab-delimited text, variable short name as header # Missing_Values: na Taxa ID Region Depth Lat_N Long_W Skel_d13C Polyp_d13C_lipids Polyp_w/o_lipids Skel_d15N Polyp_lipids_d15N Skel_C/N Polyp_lipids_C/N Polyp_w/o_lipids_C/N Ala_P_d13C Ala_P_d13C_1s Asp_P_d13C Asp_P_d13C_1s Glu_P_d13C Glu_P_d13C_1s Gly_P_d13C Gly_P_d13C_1s Ile_P_d13C Ile_P_d13C_1s Leu_P_d13C Leu_P_d13C_1s Phe_P_d13C Phe_P_d13C_1s Pro_P_d13C Pro_P_d13C_1s Ser_P_d13C Ser_P_d13C_1s Thr_P_d13C Thr_P_d13C_1s Val_P_d13C Val_P_d13C_1s Ala_d15N Ala_d15N_1s Asp_P_d15N Asp_P_d15N_1s Glu_P_d15N Glu_P_d15N_1s Gly_P_d15N Gly_P_d15N_1s Ile_P_d15N Ile_P_d15N_1s Leu_P_d15N Leu_P_d15N_1s Lys_P_d15N Lys_P_d15N_1s Met_P_d15N Met_P_d15N_1s Phe_P_d15N Phe_P_d15N_1s Pro_P_d15N Pro_P_d15N_1s Ser_P_d15N Ser_P_d15N_1s Thr_P_d15N Thr_P_d15N_1s Val_P_d15N Val_P_d15N_1s Ala_Skel_d13C Ala_Skel_d13C_1s Asp_Skel_d13C Asp_Skel_d13C_1s Glu_Skel_d13C Glu_Skel_d13C_1s Gly_Skel_d13C Gly_Skel_d13C_1s Ile_Skel_d13C Ile_Skel_d13C_1s Leu_Skel_d13C Leu_Skel_d13C_1s Phe_Skel_d13C Phe_Skel_d13C_1s Pro_Skel_d13C Pro_Skel_d13C_1s Ser_Skel_d13C Ser_Skel_d13C_1s Thr_Skel_d13C Thr_Skel_d13C_1s Val_Skel_d13C Val_Skel_d13C_1s Ala_Skel_d15N Ala_Skel_d15N_1s Asp_Skel_d15N Asp_Skel_d15N_1s Glu_Skel_d15N Glu_Skel_d15N_1s Gly_Skel_d15N Gly_Skel_d15N_1s Ile_Skel_d15N Ile_Skel_d15N_1s Leu_Skel_d15N Leu_Skel_d15N_1s Lys_Skel_d15N Lys_Skel_d15N_1s Met_Skel_d15N Met_Skel_d15N_1s Phe_Skel_d15N Phe_Skel_d15N_1s Pro_Skel_d15N Pro_Skel_d15N_1s Ser_Skel_d15N Ser_Skel_d15N_1s Thr_Skel_d15N Thr_Skel_d15N_1s Val_Skel_d15N Val_Skel_d15N_1s Primnoa pacifica GOA-13-004 Gulf of Alaska 25 58.3 149.5 -17.3 -19.5 -16.3 11.8 15.4 3.1 5.3 3.2 -18.4 0.2 -14.6 0.5 -19.4 0.5 -6.0 0.1 -15.3 0.2 -24.0 0.6 -18.3 0.6 -12.6 0.7 -0.4 0.6 -6.2 0.3 -22.4 0.5 24.7 0.1 18.5 0.7 22.9 0.2 11.3 0.1 24.2 0.5 24.9 0.1 7.3 0.6 6.2 0.2 7.8 0.3 24.4 0.2 11.9 0.1 -16.4 0.6 26.5 0.6 -18.3 0.2 -14.0 0.2 -19.1 0.1 -6.8 0.1 -15.9 0.2 -24.8 0.2 -19.2 0.5 -14.0 0.6 -0.8 0.4 -6.0 0.1 -23.0 0.3 21.1 0.3 14.8 0.1 18.9 0.6 11.9 0.2 22.3 0.1 21.1 0.2 8.2 0.4 7.0 0.3 8.0 0.2 21.3 0.4 11.8 0.2 -12.8 0.5 24.7 0.3 Primnoa pacifica GOA-13-005 Gulf of Alaska 23 59.5 145.3 -15.4 -18.5 -14.9 10.9 12.6 3.3 6.8 3.4 -18.7 0.2 -13.5 0.6 -17.0 0.2 -6.2 0.1 -15.8 0.5 -24.8 0.3 -19.1 0.2 -13.0 0.5 -1.0 0.1 -6.2 0.2 -22.0 0.3 21.2 0.2 15.8 0.2 19.5 0.2 10.1 0.5 20.1 0.1 20.6 0.4 6.6 0.5 6.0 0.4 6.6 0.1 21.2 0.7 10.7 0.3 -14.2 0.0 22.8 0.3 -18.4 0.6 -13.7 0.5 -17.1 0.3 -6.7 0.5 -16.1 0.6 -24.3 0.8 -18.7 0.1 -13.6 0.5 -0.3 0.2 -6.1 0.6 -22.2 0.1 18.6 0.1 12.7 0.5 16.7 0.2 10.7 0.8 18.1 0.2 17.6 0.5 7.2 0.1 6.4 0.1 6.9 0.5 18.2 0.5 11.4 0.6 -11.4 0.2 20.2 0.1 Primnoa pacifica GOA-13-011 Gulf of Alaska 191 56.2 135.1 -15.3 -20.3 -15.4 11.2 13.3 3.4 4.5 3.2 -18.6 0.2 -14.9 0.6 -17.8 0.6 -6.3 0.2 -16.5 0.2 -25.4 0.2 -19.9 0.7 -13.6 0.6 0.0 0.5 -6.2 0.4 -22.7 0.1 22.1 0.5 16.5 0.1 20.6 0.6 11.4 0.2 20.8 0.4 21.1 0.6 7.5 0.5 7.2 0.1 7.3 0.6 23.1 0.2 11.3 0.7 -15.4 0.1 23.6 0.1 -19.9 0.5 -15.9 0.7 -19.0 0.5 -6.4 0.1 -16.4 0.3 -25.0 0.2 -19.5 0.4 -13.6 0.2 0.4 0.2 -7.6 0.2 -21.9 0.6 19.4 0.4 13.1 0.6 16.9 0.6 12.2 0.1 18.0 0.2 19.0 0.1 7.5 0.0 6.6 0.2 6.9 0.1 19.5 0.5 10.8 0.6 -11.7 0.6 21.5 0.4 Primnoa pacifica GOA-13-046 Gulf of Alaska 165 56.2 135.1 -14.1 -20.3 -14.7 10.8 13.6 2.9 4.2 3.4 -19.5 0.5 -15.3 0.4 -19.3 0.7 -6.5 0.1 -17.1 0.1 -25.0 0.4 -18.0 0.4 -14.4 0.5 -0.2 0.2 -6.5 0.1 -22.7 0.2 23.7 0.6 16.8 0.2 21.9 0.2 10.6 0.1 21.5 0.3 22.4 0.2 7.8 0.6 8.3 0.4 6.8 0.6 23.2 0.1 11.7 0.6 -16.2 0.2 24.4 0.1 -19.9 0.4 -15.3 0.1 -19.1 0.4 -6.8 0.2 -16.1 0.2 -24.6 0.2 -18.4 0.1 -15.6 0.2 -1.3 0.1 -6.9 0.2 -21.8 0.5 20.2 0.5 13.8 0.5 18.8 0.1 11.0 0.1 19.3 0.6 19.5 0.1 8.2 0.5 8.0 0.2 6.8 0.2 20.6 0.3 12.4 0.5 -13.2 0.1 23.0 0.5 Primnoa pacifica GOA-GB2 Gulf of Alaska na 58.9 136.8 -15.8 -19.7 -15.3 11.1 14.1 2.8 4.8 3.5 -20.6 0.2 -16.2 0.1 -19.6 0.5 -5.6 0.2 -16.6 0.5 -24.8 0.2 -18.0 0.2 -10.8 0.6 -1.7 0.6 -5.6 0.7 -22.1 0.6 20.9 0.2 17.3 0.7 20.0 0.4 11.6 0.4 20.9 0.7 21.8 0.1 7.6 0.4 6.8 0.2 7.9 0.1 22.6 0.1 12.4 0.5 -14.5 0.4 24.0 0.1 -19.7 0.2 -16.2 0.4 -20.2 0.4 -5.3 0.6 -16.3 0.7 -24.0 0.5 -18.4 0.5 -12.5 0.7 -1.3 0.4 -6.6 0.2 -22.1 0.4 17.8 0.1 14.5 0.6 16.6 0.2 12.8 0.6 18.4 0.5 18.9 0.4 7.0 0.4 7.2 0.5 7.4 0.6 20.1 0.6 13.5 0.8 -10.6 0.1 22.2 0.1 Isidella spp D620#3 Central California Margin 1224 36.4 122.3 -15.1 -17.9 -14.6 14 14.8 2.6 4.5 3.2 -17.1 0.1 -12.5 0.6 -16.7 0.2 -3.9 0.5 -14.6 0.4 -23.0 0.3 -17.3 0.1 -11.9 0.1 1.8 0.2 -3.7 0.3 -19.9 0.1 26.6 0.6 21.4 0.4 25.1 0.1 13.2 0.3 24.0 0.2 25.7 0.1 8.8 0.1 8.2 0.4 9.8 0.0 26.2 0.5 13.0 0.6 -11.6 0.6 27.3 0.2 -17.8 0.6 -12.8 0.6 -16.9 0.6 -3.8 0.4 -15.6 0.1 -23.4 0.2 -17.7 0.1 -12.5 0.5 1.5 0.3 -3.5 0.4 -19.6 0.4 22.3 0.1 17.5 0.2 21.2 0.1 14.4 0.4 20.6 0.2 22.3 0.2 9.1 0.4 na na 9.7 0.3 22.0 0.2 13.2 0.5 -8.9 0.2 24.5 0.1 Isidella spp D620#4 Central California Margin 1127 36.4 122.3 -16.1 -18.3 -15.3 15.2 16.4 2.7 4.7 2.9 -17.7 0.2 -11.9 0.5 -16.6 0.6 -3.5 0.1 -15.9 0.5 -22.9 0.4 -16.9 0.2 -11.3 0.4 2.2 0.1 -5.1 0.2 -19.6 0.2 26.6 0.5 20.6 0.5 23.6 0.5 13.0 0.2 24.8 0.5 25.9 0.1 9.2 0.6 9.5 0.2 9.5 0.2 26.9 0.5 13.6 0.7 -12.0 0.3 27.1 0.4 -16.9 0.7 -12.6 0.2 -17.3 0.2 -3.7 0.5 -16.1 0.6 -22.7 0.2 -16.8 0.2 -11.0 0.2 1.5 0.1 -4.4 0.2 -19.7 0.5 22.8 0.1 17.6 0.5 20.8 0.6 14.7 0.1 21.0 0.2 22.2 0.7 9.6 0.0 na na 9.4 0.2 22.9 0.8 14.7 0.0 -9.4 0.1 24.0 0.1 Isidella spp D639#2 Central California Margin 1230 36.4 122.3 -15.9 -18.8 -15.4 15.1 16.3 3.3 4.8 3.2 -16.8 0.4 -13.1 0.6 -17.0 0.6 -4.7 0.6 -15.7 0.4 -21.6 0.5 -17.2 0.1 -12.7 0.1 2.0 0.3 -3.7 0.5 -20.3 0.1 25.7 0.4 21.1 0.4 24.1 0.6 13.2 0.3 23.3 0.3 26.1 0.1 9.9 0.3 10.1 0.5 10.7 0.5 26.9 0.5 14.8 0.5 -11.7 0.5 26.1 0.4 -18.0 0.4 -12.4 0.5 -16.5 0.2 -3.7 0.2 -15.2 0.1 -21.8 0.5 -16.3 0.5 -12.1 0.7 2.3 0.1 -4.0 0.1 -20.7 0.1 22.6 0.7 17.5 0.3 21.2 0.2 14.2 0.6 20.2 0.4 23.2 0.5 9.6 0.6 na na 10.4 0.6 23.3 0.8 15.4 0.1 -8.6 0.4 24.4 0.4 Isidella spp D641#1 Central California Margin 1247 36.4 122.3 -15.2 -18 -14.6 14.4 16.2 2.6 4.8 2.8 -17.4 0.3 -12.4 0.5 -16.7 0.1 -4.6 0.4 -15.4 0.6 -23.3 0.2 -16.2 0.6 -12.0 0.1 1.8 0.6 -5.9 0.1 -17.9 0.4 25.5 0.4 21.6 0.1 24.1 0.1 14.2 0.2 23.7 0.7 25.1 0.2 10.1 0.2 10.3 0.6 10.3 0.5 25.9 0.6 15.6 0.4 -10.8 0.5 25.7 0.4 -16.9 0.4 -13.7 0.5 -17.1 0.5 -4.4 0.2 -14.5 0.2 -23.7 0.6 -16.4 0.1 -12.2 0.5 1.3 0.6 -5.2 0.5 -17.2 0.3 21.4 0.6 17.5 0.6 20.7 0.5 15.8 0.4 19.9 0.5 20.9 0.2 10.7 0.2 na na 10.3 0.4 21.9 0.6 16.6 0.2 -9.2 0.3 24.1 0.2 Isidella spp D641#2 Central California Margin 1248 36.4 122.3 -15.1 -19.7 -15.5 15.3 16.3 2.7 5.1 3.3 -17.3 0.5 -12.0 0.2 -16.7 0.4 -4.7 0.5 -16.0 0.2 -21.5 0.0 -16.7 0.5 -10.9 0.7 1.3 0.2 -4.1 0.2 -20.7 0.4 26.7 0.4 21.8 0.5 25.0 0.2 13.4 0.2 24.5 0.6 27.2 0.3 10.2 0.2 10.1 0.2 10.8 0.7 27.6 0.3 14.3 0.1 -12.2 0.2 27.5 0.2 -17.6 0.1 -11.7 0.6 -16.8 0.4 -4.6 0.3 -15.1 0.2 -22.0 0.1 -16.4 0.6 -11.0 0.2 2.3 0.8 -3.8 0.6 -20.5 0.5 22.2 0.1 17.3 0.2 21.3 0.3 14.6 0.4 20.4 0.5 22.6 0.2 10.7 0.4 na na 10.4 0.6 23.6 0.8 14.9 0.1 -9.5 0.2 25.7 0.2 Kulamanamana haumeaae PV588Ger13 North Pacific Subtopical Gyre 404 18.7 158.3 -16.9 -20.5 -16.5 8.1 10.5 3.2 4.4 2.8 -15.6 0.1 -17.7 0.1 -19.1 0.1 -0.4 0.7 -22.3 0.4 -28.8 0.2 -30.9 0.4 -17.4 0.5 1.7 0.1 -12.9 0.4 -27.3 0.1 23.3 0.4 14.3 0.1 19.0 0.2 7.7 0.2 22.0 0.1 23.9 0.6 2.1 0.3 2.9 0.1 2.1 0.4 25.1 0.1 7.8 0.2 -18.4 0.2 24.3 0.3 -16.4 0.4 -16.2 0.1 -18.3 0.1 0.5 0.6 -22.0 0.1 -29.6 0.3 -30.3 0.1 -16.0 0.3 2.3 0.1 -12.6 0.5 -27.6 0.1 19.6 0.2 11.0 0.4 15.5 0.5 8.3 0.4 18.4 0.6 19.8 0.1 2.6 0.2 na na 2.6 0.8 21.5 0.2 8.6 0.5 -16.4 0.5 22.3 0.6 Kulamanamana haumeaae PV588Ger11 North Pacific Subtopical Gyre 394 18.7 158.3 -16.9 -20.4 -16.4 8.6 9.9 2.8 4.3 2.8 -16.2 0.8 -17.5 0.4 -19.3 0.3 -1.5 0.4 -22.8 0.3 -29.5 0.5 -32.4 0.8 -16.0 0.2 2.1 0.6 -15.2 0.5 -26.2 0.2 23.0 0.2 12.7 0.2 18.6 0.1 7.2 0.2 21.9 0.1 23.6 0.1 2.4 0.2 2.9 0.6 2.8 0.5 25.3 0.7 8.4 0.1 -19.4 0.1 24.5 0.2 -16.6 0.1 -17.2 0.6 -19.2 0.1 -0.2 0.1 -22.8 0.4 -29.8 0.4 -31.3 0.3 -16.9 0.6 1.6 0.1 -14.8 0.4 -28.1 0.4 19.3 0.4 9.9 0.3 15.0 0.2 8.0 0.1 18.5 0.1 20.3 0.3 2.5 0.3 na na 2.8 0.5 22.2 0.5 8.4 0.4 -16.9 0.6 22.3 0.1 Kulamanamana haumeaae PV694Ger14 North Pacific Subtopical Gyre 356 23.9 165.4 -17 -20.3 -16.4 8.2 10.4 2.8 4.1 2.8 -15.8 0.1 -14.7 0.1 -17.4 0.6 0.3 0.2 -20.9 0.1 -30.4 0.4 -30.7 0.6 -16.4 0.2 1.2 0.5 -14.1 0.3 -27.1 0.1 22.0 0.5 12.2 0.5 17.5 0.3 7.6 0.5 19.8 0.4 22.6 0.2 2.5 0.1 2.7 0.5 2.7 0.1 24.4 0.2 9.1 0.6 -19.8 0.6 23.1 0.1 -17.2 0.1 -14.1 0.3 -16.2 0.2 0.7 0.2 -20.8 0.6 -30.2 0.0 -31.1 0.5 -16.0 0.5 2.8 0.2 -13.2 0.6 -27.5 0.6 19.1 0.3 9.1 0.6 14.3 0.4 8.7 0.1 16.8 0.1 19.0 0.1 2.6 0.6 na na 2.3 0.4 21.2 0.2 9.2 0.3 -17.3 0.2 20.6 0.2