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OAS accession Detail for 0112364, meta_version: 8. Current meta_version is: 10
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| accessions_id: | 0112364 | archive |
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| Title: | Bacteria abundance, biomass and chlorophyll a in ice Cores: data collected in the South Atlantic Ocean and South Pacific Ocean on the LAURENCE M. GOULD and NATHANIEL B. PALMER cruises LMG0106, LMG0205 and others as part of the Southern Ocean GLOBEC project from 2001-07-28 to 2002-09-11 (NCEI Accession 0112364) |
| Abstract: | This dataset includes biological and ice core data collected aboard the LAURENCE M. GOULD and NATHANIEL B. PALMER during cruises LMG0106, LMG0205, NBP0104 and NBP0204 in the South Atlantic Ocean and South Pacific Ocean from 2001-07-28 to 2002-09-11. These data include CHLOROPHYLL A and BACTERIA - BACTERIAL DENSITY. The instruments used to collect these data include ice corer. These data were collected by Christian H. Fritsen of University and Community College System of Nevada as part of Southern Ocean GLOBEC. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NODC on 2013-07-23. The following is the text of the abstract provided by BCO-DMO: Bacteria Abundance, Biomass and Chlorophyll a in Ice Cores NOTES: NBP0104: Cores labeled with "DNA" were collected for DNA analysis. Contributor: Dr. Christian Fritsen University and Community College System of Nevada Desert Research Institute Div. of Earth and Ecosystem Sciences 2215 Raggio Parkway Reno, NV 89512 Office: 775/673-7487 BG 235 - Methods used for chlorophyll a (chla) analysis and bacteria biomass determination Core Sampling techniques: Sampling methods for recovery of chlorophyll a and bacteria from sea ice cores follows those described in: Garrison, D.L. and K.R. Buck (1986), Organism losses during ice melting: a serious bias in sea ice community studies. Polar Biol., 6:237-239. Recommendations for reporting were used as outlined by: Horner, R. et al. (1992), Ecology of Sea Ice Biota. I: Habitat, Terminology and Methodology. Polar Biol. 12:417-427 Analytic Techniques: Chla (mg m-3): determined fluorometrically (Turner Designs 10AU Fluorometer) following extraction in 90% acetone (Parsons et al., 1984). ice core chla corrected to account for chla in filtered sea water (FSW) added to core sections during melting Bacteria cell abundance (cells m-3) and biomass (mg C m-3): LMG 0106. preserved (0.5% glutaraldehyde) samples stained with 4',6-diamidino-2-phenylindole (DAPI; 0.1 to 0.3% final concentration), filtered through 0.2 mm black, polycarbonate membrane filters, and mounted onto glass microscope slides on the ship (within 24 hours following collection) bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus bacteria biomass determined using cell abundance, cell biovolume (BV; mm3; as determined from mean length and width measurements), and an allometric conversion factor for bacterial carbon per volume specific for DAPI-stained bacteria (cellular carbon = 218 X BV0.86; Loferer-Kribacher et al., 1998). ice core samples corrected for FSW dilution NBP 0104 preserved (0.5% glutaraldehyde) samples stained with Sybri Gold (0.01% final concentration), filtered through 0.2 mm Anodisc filters (Whatman), and mounted onto glass microscope slides at home institution (~1-2 months following collection) bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus bacteria biomass determined using cell abundance, cell biovolume (BV; mm3), and an allometric conversion factor for bacterial carbon per volume specific for Acridine Orange-stained bacteria (cellular carbon = 89.9 X BV0.59; Simon and Azam, 1989). Note: an AO-specific carbon per volume conversion factor was used in calculating biomass in Sybri Gold-stained samples because both AO and Sybri Gold stain bacteria cells similarly relative to DAPI (unpublished data). ice core samples corrected for FSW dilution Loferer-Kribacher, M., Klima, J., and R. Psenner. 1998. Determination of bacterial cell dry mass by transmission electron microscopy and densitometric image analysis. Applied and Environmental Microbiology. 64:688-694. Parsons,T.R., Maita, Y., and C.M. Lalli. 1984. A manual of chemical and biological methods for seawater analysis. Pergamon Press. Elmsford, New York. Simon, M., and F. Azam. 1989. Protein content and protein synthesis rates of planktonic marine bacteria. Marine Ecology Progress Series. 51, 201-213. updated: April 20, 2006 |
| Date received: | 20130723 |
| Start date: | 20010728 |
| End date: | 20020911 |
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| West boundary: | -76.781 |
| East boundary: | -65.61 |
| North boundary: | -65.62 |
| South boundary: | -69.25 |
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| Submitter: | DuBois, David L. |
| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
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| Supplementary information: | The Data Originator(s)/Principal Investigator(s) for these data: Christian H. Fritsen, University and Community The data were collected during the following cruise(s): LMG0106 LMG0205 NBP0104 NBP0204 The collection of the data was supported by: NSF Division of Antarctic Sciences |
| Availability date: | |
| Metadata version: | 8 |
| Keydate: | 2013-08-23 13:45:05+00 |
| Editdate: | 2023-01-27 02:46:49+00 |