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OAS accession Detail for 0277903
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| accessions_id: | 0277903 | archive |
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| Title: | Symbiodinium cultures isolated from the octocoral Antillogorgia bipinnata in the Florida Keys and processed at Coffroth lab at the University at Buffalo in 2008, 2013 and 2016 (Host Symbiont Temp Response project) (NCEI Accession 0277903) |
| Abstract: | This dataset contains biological and survey - biological data collected at SUNY-Buffalo during deployment Coffroth_2008-16 from 2008-01-01 to 2016-12-31. These data include species, stage, and taxon. The instruments used to collect these data include Automated DNA Sequencer and Centrifuge. These data were collected by Casey terHorst of California State University Northridge and Mary Alice Coffroth of State University of New York at Buffalo as part of the "RUI: Collaborative Research: Genetic variation as a driver of host and symbiont response to increased temperature on coral reefs (Host Symbiont Temp Response)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-10-02. The following is the text of the dataset description provided by BCO-DMO: Symbiodinium cultures isolated from adult colonies of the octocoral Antillogorgia bipinnata in 2008, 2013 and 2016, grown at either 26 or 30 degrees C Dataset Description: Symbiodinium cultures isolated from adult colonies of the octocoral Antillogorgia bipinnata in 2008, 2013 and 2016, grown at either 26 or 30 degrees C. Cultures initially isolated from Antillogorgia bipinnata colonies collected in the lower keys in the vicinity of Looe Key (24 32.973'N 81 22.849'W) in 2008, the middle keys near Tennessee Reef (24 45.150'N 81 45.275'W) in 2013 and the upper keys at Pickles Reef (24 59.016'N 80 24.832'W) and Elbow Reef (25 07.956'N 80 15.810'W and 25 07.925'N 80 15.717'W). Culture have been maintained in the Coffroth lab, University at Buffalo. |
| Date received: | 20191002 |
| Start date: | 20080101 |
| End date: | 20161231 |
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| West boundary: | -81.75458 |
| East boundary: | -80.26195 |
| North boundary: | 25.1326 |
| South boundary: | 24.54955 |
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| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
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| Supplementary information: | Acquisition Description: Symbionts were isolated from adult colonies of Antillogorgia bipinnata following the protocol outline in Santos et al 2001. Briefly, a small piece (1-2 cm) of the branch was ground in a glass tissue homogenizer with 2 ml of filtered seawater (FSW) and poured through a series of meshes (250 µm on top, then 120 µm, then 70 µm mesh) into a 15 ml tube. The mesh was washed with 1 ml FSW several times for a final volume between 3 and 10 ml. This slurry was spun for 5 min at 500-800 rpm on a Beckman J6-HC centrifuge to pellet symbiont cells, the supernatant removed and resuspended in 10 ml of FSW. This step was repeated again and then the pellet was resuspended in 1.0 ml of F/2 (Gulliard and Ryther 1962). Cultures were started by using 20-50 µl of the resuspended pellets to inoculate 30ml of F/2 and incubated at the appropriate temperature. Clade identity and Cp-type were determined following the protocols outline in Santos et al (2003). Briefly, DNA was amplified using the primers HYPERUP and HYPERDN on and MJ96 or BioRad thermocyclers. PCR products were visualized and scored using size standards on a LI-COR 4200 NEN® Global IR2 DNA sequencing system as specified in Santos et al (2003). Putative species identity was based on sequence analysis of B7SYM15 flanking region (LaJeunesse et al. 2012, Parkinson et al. 2015). Briefly, the B7SYM15 flanking region was amplified and directly sequenced in 5’ and 3’ directions on a 3730XL DNA Analyzer (High Throughput Genomics Center, University of Washington). Sequences were compared to known species within the GenBank database using BLAST- Basic Local Alignment Search Tool ( https://blast.ncbi.nlm.nih.gov/Blast.cgi ). |
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| Metadata version: | 1 |
| Keydate: | 2023-05-05 04:33:28+00 |
| Editdate: | 2023-05-05 04:33:49+00 |