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OAS accession Detail for 0278843, meta_version: 1. Current meta_version is: 1
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accessions_id: | 0278843 | archive |
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Title: | Otolith microstructure of young-of-year Atlantic silversides (Menidia menidia) from Mumford Cove during 2015 (NCEI Accession 0278843) |
Abstract: | This dataset contains biological, physical, and survey - biological data collected at lab Avery_Point during deployment Mumford_Cove from 2015-05-28 to 2015-12-18. These data include growth, sex, species, and water temperature. The instruments used to collect these data include Camera, Microscope-Optical, and Water Quality Multiprobe. These data were collected by Hannes Baumann of University of Connecticut as part of the "Collaborative research: Understanding the effects of acidification and hypoxia within and across generations in a coastal marine fish (HYPOA)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-11-20. The following is the text of the dataset description provided by BCO-DMO: Otolith microstructure of young-of-year Atlantic silversides from Mumford Cove 2015 Dataset Description: Back-calculated age, hatch date, length-at-age, growth rate, and experienced temperature of young-of-year Atlantic silversides (Menidia menidia) collected during 3 successive collections in Mumford Cove, CT in fall 2015. |
Date received: | 20191120 |
Start date: | 20150528 |
End date: | 20151218 |
Seanames: | |
West boundary: | -72.02 |
East boundary: | -72.02 |
North boundary: | 41.32 |
South boundary: | 41.32 |
Observation types: | biological, physical, survey - biological |
Instrument types: | camera, microscope |
Datatypes: | growth rate, SPECIES IDENTIFICATION, SPECIES IDENTIFICATION - SEX, WATER TEMPERATURE |
Submitter: | |
Submitting institution: | Biological and Chemical Oceanography Data Management Office |
Collecting institutions: | University of Connecticut |
Contributing projects: | |
Platforms: | |
Number of observations: | |
Supplementary information: | Acquisition Description: Young-of-year (YoY) Atlantic silversides were collected from Mumford Cove (41.32N, 72.02W), a small, salt-marsh dominated embayment in eastern Long Island Sound (Connecticut USA, 0.5 km², 1-3 m depth). From a biweekly beach seine survey (30×2m seine with 3mm mesh), three samples on 23 October, 20 November, and 18 December 2015 were used. On each date, samples were pooled from two independent seine casts. All silversides were euthanized, enumerated, measured for total length (TL) to the lower 5 mm and preserved frozen (-20°C). From each collection, ~100 individuals were selected for otolith microstructure analysis using a TL-stratified random sampling design. For each individual, we again measured TL (nearest 0.1 mm) and determined its sex via visual inspection of gonads, before extracting both sagittal otoliths. Sagittae were mounted on microscope slides using CrystalBond 509 thermoplastic cement. If equally suitable, the left or right sagitta was randomly chosen for analysis. Otoliths were hand-polished using 9 µm then 3 µm lapping films (3M) until daily increments along the entire reading axis were clearly visible under 400x magnification (Nikon Eclipse E400 compound microscope). Otoliths were measured and read across the sagittal plane from the nucleus to the dorsal or ventral otolith edge, because the more conventional core to post-rostrum axis proved to be too curved to be reliably interpretable. Increments were enumerated and measured using Image Pro Premier (V9.1) connected to a Luminera Infinity2-2 digital camera. For each otolith section, multiple focal planes were captured and merged into multi-layer images to aid the reader interpreting the otolith microstructure. The radius of the hatch-check (µm) was measured. The last growth increment was presumed to be incomplete and thus excluded from growth analyses. Increment number was assumed to correspond to an individuals’ age in days post hatch (dph). Hatch date was calculated by subtracting age from the date of collection, while the formation date of each increment was calculated by adding the increment number to the hatch date. To estimate the thermal history of October YoY we used continuous temperature data recorded by a Manta Sub2 probe (Eureka© Water Probes) at our study site. Three small gaps in the record ( |
Availability date: | |
Metadata version: | 1 |
Keydate: | 2023-05-28 04:19:26+00 |
Editdate: | 2023-05-28 04:19:47+00 |