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OAS accession Detail for 0288337
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| accessions_id: | 0288337 | archive |
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| Title: | Changes in biovolume in three herbivorous protists measured across a temperature gradient ranging from 0 to 22 degrees Celsius for 30 days from 2017-04-01 to 2017-06-30 (NCEI Accession 0288337) |
| Abstract: | This dataset contains biological and survey - biological data collected from 2017-04-01 to 2017-06-30. These data include species. The instruments used to collect these data include Camera, In-situ incubator, and Microscope - Optical. These data were collected by Gayantonia Franzè and Susanne Menden-Deuer of University of Rhode Island as part of the "Quantifying Temperature Dependence In Growth & Grazing Rates of Planktonic Herbivores (Planktonic Herbivore Temp Dependence)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2021-08-24. The following is the text of the dataset description provided by BCO-DMO: Temperature dependent changes in biovolume of three herbivorous protists Dataset Description: Acquisition Description: See complete methodology in Franzè and Menden-Deuer, 2020. Clonal cultures of Oxyrrhis marina (CCMP 3375), Gyrodinium dominans (SPMC 103), and Protoperidinium bipes were established from single-cell isolation. Herbivorous protists were acclimated to each target temperature before growth rates were measured. Due to the wide temperature range tested (0−22°C), cultures were moved from the initial temperature of 15°C through gradual transitions limited to at most 3°C. The experimental Day 0 (D0) was defined as the first day on which acclimated growth rates were measured. To determine predator and prey abundance, samples were preserved in acid Lugol at a 2% final concentration (Menden-Deuer et al. 2001) and enumerated using a Sedgwick-Rafter slide (1 ml volume) and a Nikon Eclipse E800 light microscope equipped with phase contrast. Herbivore biovolume was calculated based on linear dimensions obtained from ≥35 cells measured at each time point and temperature treatment using an image analysis system consisting of a high-resolution digital camera (Allied Vision, Stingray F45) and ImageJ software (version 1.5i). Growth rates of the 3 herbivorous species were determined at 7 incubation temperatures: 0, 2, 5, 10, 15, 18, and 22°C. Changes in O. marina, G. dominans, and P. bipes abundance and biomass were determined over 24 h incubations of the triplicate 150 ml bottles and used to calculate growth rates (μ d−1) assuming exponential growth: μ = ln(Nt/N0)/t, where: N0 and Nt are the initial and final cell abundance (or biomass) respectively, and t is the experiment duration in days. Measurements were made at Day 0 and again after 10 and 30 days. |
| Date received: | 20210824 |
| Start date: | 20170401 |
| End date: | 20170630 |
| Seanames: | |
| West boundary: | -71.422211 |
| East boundary: | -71.422211 |
| North boundary: | 41.492779 |
| South boundary: | 41.492779 |
| Observation types: | biological, survey - biological |
| Instrument types: | camera, microscope |
| Datatypes: | SPECIES IDENTIFICATION |
| Submitter: | |
| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
| Collecting institutions: | The University of Rhode Island |
| Contributing projects: | |
| Platforms: | |
| Number of observations: | |
| Supplementary information: | |
| Availability date: | |
| Metadata version: | 2 |
| Keydate: | 2024-02-02 15:42:53+00 |
| Editdate: | 2025-01-27 22:06:03+00 |