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OAS accession Detail for 0292216
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Title: PUA (polyunsaturated aldehydes) experiments: Experimental Conditions, Virginia Coastal Bays and Bay of Napoli, Mar-July 2015 (NCEI Accession 0292216)
Abstract: This dataset contains biological and physical data collected in the Mediterranean Sea, North Atlantic Ocean, and Tyrrhenian Sea from 2015-03-23 to 2015-07-02. These data include chlorophyll a, salinity calculated from CTD primary sensors, taxon, and water temperature. The instruments used to collect these data include Water Temperature Sensor. These data were collected by Dr Peter Lavrentyev of University of Akron and Dr Diane Stoecker and Dr James J. Pierson of University of Maryland Center for Environmental Science as part of the "The effects of diatom-produced polyunsaturated aldehydes on the microbial food web in temperate and polar waters (DiatomAldehydes)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2019-10-09.

The following is the text of the dataset description provided by BCO-DMO:

PUA experimental design

Dataset Description:
This dataset describes the experimental conditions of copepods, bacteria, dinoflagellates, and ciliates under varying environmental conditions and concentrations of particulate and dissolved polyunsaturated aldehydes (PUA).
Date received: 20191009
Start date: 20150323
End date: 20150702
Seanames: Mediterranean Sea, North Atlantic Ocean, Tyrrhenian Sea
West boundary: -75.987
East boundary: 14.25
North boundary: 40.808
South boundary: 37.166
Observation types: biological, physical
Instrument types: temperature sensor
Datatypes: CHLOROPHYLL A, SALINITY, TAXONOMIC CODE, WATER TEMPERATURE
Submitter:
Submitting institution: Biological and Chemical Oceanography Data Management Office
Collecting institutions: University of Maryland Center for Environmental Science
Contributing projects:
Platforms:
Number of observations:
Supplementary information: Acquisition Description:
Data for experimental conditions were collected from the station locations where water and zooplankton were collected. Temperature and salinity were determined using a YSI ProDO2020.

Laboratory experiments were conducted by collecting raw seawater, filtering it through 200µm mesh sieves into 20L carboys, and then dispensing it into experimental jars. Triplicates bottles were used for each treatment. Treatments included whole seawater (control), whole seawater plus copepods (Zooplankton), and the same treatments plus polyunsaturated aldehydes (PUA) additions (Heptadienal, Octadienal, Decadienal, and Mixed PUA). PUA were dissolved in methanol and added to experimental bottles for a final concentration of 21 nM; for the mixed PUA treatment this was 7nM of each type of PUA. In addition, nutrients were added to all experimental bottles in the form of phytoplankton growth media, to ensure that autotroph growth was not limited by available nutrients in the experimental bottles.

Samples for PUA analysis were collected and processed according to the procedures described in Lavrentyev et al. 2015 & Franzé et al. 2018. These samples were then frozen and shipped to Texas for analysis.

All data were processed in Microsoft Excel.
Availability date:
Metadata version: 1
Keydate: 2024-05-02 13:08:04+00
Editdate: 2024-05-02 13:08:57+00