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OAS accession Detail for 0299439
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| accessions_id: | 0299439 | archive |
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| Title: | Post-diapause Neocalanus flemingeri females morphometric measurements and calculations of lipid fullness and lipid volume taken from the R/V Sikuliaq and the R/V Tiglax in the Northern Gulf of Alaska from 2019-06-30 to 2019-09-13 (NCEI Accession 0299439) |
| Abstract: | This dataset contains biological and survey - biological data collected on R/V Sikuliaq and R/V Tiglax during cruises SKQ201915S and TXF19 in the Gulf of Alaska from 2019-06-30 to 2019-09-13. These data include sex and species. The instruments used to collect these data include Camera, Microscope - Optical, and MultiNet. These data were collected by Daniel K. Hartline, Kira J. Monell, and Petra H. Lenz of University of Hawaii at Manoa as part of the "Collaborative Proposal: Optimizing Recruitment of Neocalanus copepods through Strategic Timing of Reproduction and Growth in the Gulf of Alaska (Neocalanus Gulf of Alaska)" and "Collaborative Research: Molecular profiling of the ecophysiology of dormancy induction in calanid copepods of the Northern Gulf of Alaska LTER site (Diapause preparation)" projects. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2024-07-10. The following is the text of the dataset description provided by BCO-DMO: Methods and Sampling: Sample collection and sorting Copepods were collected in Prince William Sound, Alaska in the summer and fall of 2019 during the Northern Gulf of Alaska Long Term Ecological Research (NGA LTER) cruises (). Females “PWS2/June” were collected on June 30th, 2019 at the sampling site PWS2 (Latitude 60° 32.1’N; Longitude 147° 48.2’W) (R/V Sikuliaq, cruise number: SKQ201915S), and the “Pleiades/September” females were collected on September 12th and 13th, 2019 at PWS2 and near the Pleiades Islands (Latitude 60° 16.7’N; Longitude 147° 59.2’W) (M/V Tiglax, cruise number: TGX201909). Copepods were collected with a Midi MultiNet (0.25 m2 mouth area; 150 μm mesh nets) towed vertically from near the bottom to the 163 surface at 0.5 m/sec (PWS2: 798 m; KIP2: 588 m). Upon retrieval, net samples were immediately diluted using filtered seawater collected from depth and kept between 4-6°C to minimize thermal stress. All females selected for the experiments were sorted under a dissecting microscope. Females were placed in groups of three into 750 mL Falcon tissue-culture flasks and incubated under dim light in an incubator for up to 4.5 weeks. Experimental emperatures were at or below deep-water temperatures in Prince William Sound (temperature settings: 4°C for June and 6°C for September). A subset of females was used in the DNA replication experiments; the remaining females were imaged for measurements of prosome length and lipid sac area (see the related dataset ). Experimental design For the timeline, sets of females were removed from the incubation flasks for imaging and/or incubations in low concentrations of 5-Ethynyl-2’-deoxyuridine (EdU). Individuals that were imaged as well as incubated in EdU are identified in the dataset and provide identifications used in the related dataset. |
| Date received: | 20240710 |
| Start date: | 20190630 |
| End date: | 20190913 |
| Seanames: | Gulf of Alaska |
| West boundary: | -147.987 |
| East boundary: | -147.803 |
| North boundary: | 60.535 |
| South boundary: | 60.278 |
| Observation types: | biological, survey - biological |
| Instrument types: | camera, microscope, net - plankton net |
| Datatypes: | SPECIES IDENTIFICATION, SPECIES IDENTIFICATION - SEX |
| Submitter: | |
| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
| Collecting institutions: | University of Hawai'i at Mānoa |
| Contributing projects: | |
| Platforms: | Sikuliaq (33BI), Tiglax (33A0) |
| Number of observations: | |
| Supplementary information: | |
| Availability date: | |
| Metadata version: | 1 |
| Keydate: | 2024-11-29 16:03:14+00 |
| Editdate: | 2024-11-29 16:03:49+00 |