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OAS accession Detail for 0112521
| << previous | |revision: 11 |
| accessions_id: | 0112521 | archive |
|---|---|
| Title: | Water column bacteria data from ARSV Laurence M. Gould cruises LMG0104 and LMG0106 in the Southern Ocean in 2001 (SOGLOBEC project; Sea Ice Microbes project) (NCEI Accession 0112521) |
| Abstract: | This dataset contains biological data collected on ARSV Laurence M. Gould during cruises LMG0104 and LMG0106 in the South Pacific Ocean and Southern Ocean from 2001-04-29 to 2001-08-20. These data include bacterial abundance, bacterial carbon biomass, chl_a_ugm, and depth. The instruments used to collect these data include Niskin bottle. These data were collected by Dr Chris H. Fritsen of Desert Research Institute as part of the "GLOBEC: Sea Ice Microbial Communities (Sea Ice Microbes)" and "U.S. GLOBEC Southern Ocean (SOGLOBEC)" projects and "U.S. GLOBal ocean ECosystems dynamics (U.S. GLOBEC)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-01-23. The following is the text of the dataset description provided by BCO-DMO: Water column bacteria data Dataset Description: Bacteria Abundance, Biomass and Chlorophyll: Water Column Samples a BG 235 - Methods used for chlorophyll a (chla) analysis and bacteria biomass determination Core Sampling techniques: Sampling methods for recovery of chlorophyll a and bacteria from sea ice cores follows those described in Garrison and Buck (1986) Recommendations for reporting were used as outlined by: Horner, R. et al.,(1992) Analytic Techniques: Chla (mg m -3 ): determined fluorometrically (Turner Designs 10AU Fluorometer) following extraction in 90% acetone (Parsons et al ., 1984) ice core chla corrected to account for chla in filtered sea water (FSW) added to core sections during melting Bacteria cell abundance (cells m -3 ) and biomass (mg C m -3 ): LMG 0106 preserved (0.5% glutaraldehyde) samples stained with 4',6-diamidino-2-phenylindole (DAPI; 0.1 to 0.3% final concentration), filtered through 0.2 mm black, polycarbonate membrane filters, and mounted onto glass microscope slides on the ship (within 24 hours following collection) bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus bacteria biomass determined using cell abundance, cell biovolume (BV; mm 3 ; as determined from mean length and width measurements), and an allometric conversion factor for bacterial carbon per volume specific for DAPI-stained bacteria (cellular carbon = 218 X BV 0.86 ; Loferer-Krossbacher et al. , 1998). ice core samples corrected for FSW dilution NBP 0104 preserved (0.5% glutaraldehyde) samples stained with SYBR Gold (0.01% final concentration), filtered through 0.2 mm Anodisc filters (Whatman), and mounted onto glass microscope slides at home institution (~1-2 months following collection) bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus bacteria biomass determined using cell abundance, cell biovolume (BV; mm 3 ), and an allometric conversion factor for bacterial carbon per volume specific for Acridine Orange-stained bacteria (cellular carbon = 89.9 X BV 0.59 ; Simon and Azam, 1989). Note: an AO-specific carbon per volume conversion factor was used in calculating biomass in SYBR Gold-stained samples because both AO and SYBR Gold stain bacteria cells similarly relative to DAPI (unpublished data). ice core samples corrected for FSW dilution Data from LMG0106 (July-August, 2001) added in June 2002. Updated: April 21, 2006 |
| Date received: | 20230123 |
| Start date: | 20010429 |
| End date: | 20010820 |
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| West boundary: | -72.427 |
| East boundary: | -67.049 |
| North boundary: | -65.965 |
| South boundary: | -69.888 |
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| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
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| Supplementary information: | Acquisition Description: # Bacteria data from Southern Ocean GLOBEC # C. Fritsen and F. Stewart # * BactAbun = bacterial abundance = cells m-3 # * BactBio = bacterial biomass = mg C m-3 = ug C l-1 # * chla = mg chla m-3 = ug chla l-1 In this accession, NCEI has archived multiple versions of these data. The latest (and best) version of these data has the largest version number. |
| Availability date: | |
| Metadata version: | 11 |
| Keydate: | 2013-08-27 13:45:04+00 |
| Editdate: | 2025-01-18 16:05:54+00 |