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OAS accession Detail for 0112521, meta_version: 2. Current meta_version is: 11
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Title: CHLOROPHYLL A and BACTERIA - BACTERIAL DENSITY profile data collected in the South Pacific Ocean on the LAURENCE M. GOULD cruises LMG0104 and LMG0106 as part of the Southern Ocean GLOBEC project from 2001-04-29 to 2001-08-20 (NODC Accession 0112521)
Abstract: NODC Accession 0112521 includes profile and biological data collected aboard the LAURENCE M. GOULD during cruises LMG0104 and LMG0106 in the South Pacific Ocean from 2001-04-29 to 2001-08-20. These data include CHLOROPHYLL A and BACTERIA - BACTERIAL DENSITY. The instruments used to collect these data include bottle. These data were collected by Christian H. Fritsen of University and Community as part of Southern Ocean GLOBEC. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NODC on 2013-07-23.

The following is the text of the abstract provided by BCO-DMO:

Bacteria Abundance, Biomass and Chlorophyll a
Water Column Samples

Contributor:

Dr. Christian Fritsen
University and Community College System of Nevada
Desert Research Institute
Div. of Earth and Ecosystem Sciences
2215 Raggio Parkway
Reno, NV 89512

Office: 775/673-7487

BG 235 - Methods used for chlorophyll a (chla) analysis and bacteria biomass determination
Core Sampling techniques:
Sampling methods for recovery of chlorophyll a and bacteria from sea ice cores follows those described in:
Garrison, D.L. and K.R. Buck(1986), Organism losses during ice melting: a serious bias in sea ice community studies. Polar Biol., 6:237-239.
Recommendations for reporting were used as outlined by:
Horner, R. et al.,(1992), Ecology of Sea Ice Biota. I: Habitat, Terminology and Methodology. Polar Biol. 12:417-427
Analytic Techniques:
Chla (mg m-3):

determined fluorometrically (Turner Designs 10AU Fluorometer) following extraction in 90% acetone (Parsons et al., 1984)
ice core chla corrected to account for chla in filtered sea water (FSW) added to core sections during melting

Bacteria cell abundance (cells m-3) and biomass (mg C m-3):
LMG 0106

preserved (0.5% glutaraldehyde) samples stained with 4',6-diamidino-2-phenylindole (DAPI; 0.1 to 0.3% final concentration), filtered through 0.2 mm black, polycarbonate membrane filters, and mounted onto glass microscope slides on the ship (within 24 hours following collection)
bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus
bacteria biomass determined using cell abundance, cell biovolume (BV; mm3; as determined from mean length and width measurements), and an allometric conversion factor for bacterial carbon per volume specific for DAPI-stained bacteria (cellular carbon = 218 X BV0.86; Loferer-Kr��bacher et al., 1998).
ice core samples corrected for FSW dilution

NBP 0104

preserved (0.5% glutaraldehyde) samples stained with Sybr� Gold (0.01% final concentration), filtered through 0.2 mm Anodisc filters (Whatman�), and mounted onto glass microscope slides at home institution (~1-2 months following collection)
bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus
bacteria biomass determined using cell abundance, cell biovolume (BV; mm3), and an allometric conversion factor for bacterial carbon per volume specific for Acridine Orange-stained bacteria (cellular carbon = 89.9 X BV0.59; Simon and Azam, 1989). Note: an AO-specific carbon per volume conversion factor was used in calculating biomass in Sybr� Gold-stained samples because both AO and Sybr� Gold stain bacteria cells similarly relative to DAPI (unpublished data).
ice core samples corrected for FSW dilution

Loferer-Kr��bacher, M., Klima, J., and R. Psenner. 1998. Determination of bacterial cell dry mass by transmission electron microscopy and densitometric image analysis. Applied and Environmental Microbiology. 64:688-694.
Parsons,T.R., Maita, Y., and C.M. Lalli. 1984. A manual of chemical and biological methods for seawater analysis. Pergamon Press. Elmsford, New York.
Simon, M., and F. Azam. 1989. Protein content and protein synthesis rates of planktonic marine bacteria. Marine Ecology Progress Series. 51, 201-213.
Data from LMG0106 (July-August, 2001) added in June 2002.
Updated: April 21, 2006
Date received: 20130723
Start date: 20010429
End date: 20010820
Seanames:
West boundary: -72.427
East boundary: -67.049
North boundary: -65.965
South boundary: -69.888
Observation types:
Instrument types:
Datatypes:
Submitter: Chandler, Cyndy
Submitting institution: Biological and Chemical Oceanography Data Management Office
Collecting institutions:
Contributing projects:
Platforms:
Number of observations:
Supplementary information: The Data Originator(s)/Principal Investigator(s) for these data:
Christian H. Fritsen, University and Community

The data were collected during the following cruise(s):
LMG0104
LMG0106

The collection of the data was supported by:
NSF Division of Antarctic Sciences

In this accession, NODC has archived multiple versions of these data. The latest (and best) version of these data has the largest version number.
Availability date:
Metadata version: 2
Keydate: 2013-08-27 13:45:04+00
Editdate: 2013-08-29 13:45:20+00