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OAS accession Detail for 0243465
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Title: Effects of ocean acidification on the survival and growth of young-of-the-year golden king crab (Lithodes aequispinus) observed in laboratory experiments for the Effects of Ocean Acidification on Federally Managed Crab Species in Alaska project from 2014-04-14 to 2014-08-19 (NCEI Accession 0243465)
Abstract: In this study, we examined how CO2-driven acidification affected the growth and survival of juvenile golden king crab (Lithodes aequispinus), an important fishery species in Alaska. Juveniles were reared from larvae in surface ambient pH seawater at the Kodiak Laboratory. Newly molted early benthic instar crabs were randomly assigned to one of three pH treatments: (1) surface ambient pH ~ 8.2, (2) likely in situ ambient pH 7.8, and (3) pH 7.5. Thirty crabs were held in individual inserts in each treatment for 127 days and checked daily for molting or death. The complete methods, which should be read and understood prior to using this data, are published as: Long, W. C., Swiney, K. M., & Foy, R. J. (2021). Effects of ocean acidification on young of the year golden king crab (Lithodes aequispinus) survival and growth. Marine Biology, 168(8), 126. https://doi.org/10.1007/s00227-021-03930-y.
Date received: 20211118
Start date: 20140414
End date: 20140819
Seanames: Gulf of Alaska
West boundary: -152.39642
East boundary: -152.39642
North boundary: 57.781447
South boundary: 57.781447
Observation types: biological, chemical, laboratory experiments, physical
Instrument types: calipers, camera, coulometer for DIC measurement, microscope, photograph, scale, temperature probe
Datatypes: ANIMALS - INDIVIDUAL - MASS, biological data, CRUSTACEANS, DISSOLVED INORGANIC CARBON (DIC), pH, SALINITY, total alkalinity, WATER TEMPERATURE
Submitter: Long, W. Christopher
Submitting institution: US DOC; NOAA; NMFS; Alaska Fisheries Science Center
Collecting institutions: US DOC; NOAA; NMFS; Alaska Fisheries Science Center
Contributing projects: OAP, OCADS
Platforms:
Number of observations:
Supplementary information: This data package (Submission ID: BFPZ7TWGZ) was acquired by NCEI from the Scientific Data Integration System (SDIS) at the NOAA Pacific Marine Environmental Laboratory (PMEL) in accordance with the archival submission agreement between NCEI and PMEL.

Instruments:

Temperature was measured using the Durafet III probe (same as pH). Salinity was measured with a Mettler Toledo Seven Multi probe.

Crab Measurements: Each crab was checked daily for molting or mortality. When a crab molted, the exuvia was carefully removed and photographed under a stereo-microscope using a digital camera. Dead crabs were also removed and similarly photographed. Morphometric measurements, including carapace width, carapace length, rostrum base width, orbital spine width, and the first spine length, were made on the images of the crabs using Image-Pro Plus v. 7.0.1.658 (Media Cybernetics, Inc., Bethesda, Maryland, USA). The microscope was calibrated using a micrometer each time pictures were taken. At the end of the experiment all crabs were photographed for morphometric analysis as above.

Alkalinity: VINDTA 3S (Marianda, Kiel, Germany)
DIC: VINDTA 3C coupled to a 5012 Coulometer (UIC Inc., Joliet, IL)
Availability date:
Metadata version: 15
Keydate: 2021-11-23 18:38:13+00
Editdate: 2022-11-01 21:30:19+00