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OAS accession Detail for 0291731
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| accessions_id: | 0291731 | archive |
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| Title: | Volume-dependent offsets in NO3- N and O isotope ratios of reference materials (Biological Nitrogen Isotope Fractionation project) from 2019-02-20 to 2019-03-21 (NCEI Accession 0291731) |
| Abstract: | This dataset contains biological, chemical, and survey - biological data collected from 2019-02-20 to 2019-03-21. These data include Nitrate, Nitrous Oxide, d15N, species, and stable oxygen ration 18. The instruments used to collect these data include Gas Chromatograph Mass Spectrometer. These data were collected by Julie Granger of University of Connecticut as part of the "CAREER: The biological nitrogen isotope systematics of ammonium consumption and production (Biological Nitrogen Isotope Fractionation)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2022-01-14. The following is the text of the dataset description provided by BCO-DMO: Volume-dependent offsets in NO3- N and O isotope ratios of reference materials Dataset Description: Acquisition Description: Sampling and analytical procedures: 1. Analysis of NO 3 - N and O isotope ratios with the denitrifier method The denitrifying bacteria strains Pseudomonas chlororaphis f. sp. aureofaciens (ATCC 13985, Manassas, VA, USA) and Pseudomonas. chlororaphis (ATCC 43928, Manassas, VA, USA) were used. Cultures were inoculated from cryo-preserved aliquots (Weigand et al., 2011) into sterile growth media prepared as originally described (Sigman et al., 2001; Casciotti et al., 2002) in 700 mL glass bottles containing 600 mL of medium, then sealed with gas-tight lids. Cells were cultured for 7-10 days at 20˚C on a rotary shaker table. Cultures were harvested by centrifugation and resuspended into 220 mL of fresh medium without potassium nitrate addition, achieving ca. 3-fold concentration of the bacteria. Two mL of the cell concentrates were added to respective 20-mL headspace glass vials, capped with pre-rinsed butyl rubber septa and crimp-seals (McIlvin and Casciotti, 2011). Vials were sparged with a water-scrubbed N 2 gas stream for 6 hours to remove any N 2 O produced from the residual NO 3 - in the medium. NO 3 - samples were then injected into each vial to achieve a final sample size of 10 nmoles of N. Vials were incubated inverted in order to prevent potential N 2 O leakage. Following overnight incubation in the dark, ca. 0.1 ml of 10 mol L -1 NaOH was injected into each vial to kill the cultures and sequester CO 2 into carbonate species. The N 2 O gas in the vials was extracted, purified and analyzed with a Delta V Advantage continuous flow gas chromatograph isotope ratio mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) interfaced with a modified Thermo Fisher Scientific Gas Bench sample preparation device fronted by dual cold traps (Casciotti et al., 2002) and a GC Pal autosampler (CTC Analytics, Zwingen, Switzerland). Samples were referenced to pure N 2 O injections from a common reference gas cylinder. 2. Demonstration of volume effects in analyzes of NO 3 - reference materials The reference solutions were prepared from salts into primary stocks at 200 µmol L -1 in deionized water (DIW) from a Milli‐Q TM water purification system (EMD Millipore, Burlington, MA, USA), and stored frozen. Primary stocks of NO 3 - reference materials (IAEA-NO3 and USGS-34) were diluted in NO 3 - -deplete surface Sargasso seawater or in aged DIW to concentrations of 1, 5 and 20 µmol L -1 , corresponding to respective injection volumes of 10, 2 and 0.5 mL, in order to aliquot 10 nmoles of N analyte. The NO 3 - aliquots were injected into the sparged bacterial concentrates of either P. chlororaphis or P. aureofaciens . Following bacterial conversion, the resulting N 2 O in the reaction vials was extracted, purified and analyzed on the isotope ratio mass spectrometer. |
| Date received: | 20220114 |
| Start date: | 20190220 |
| End date: | 20190321 |
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| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
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| Metadata version: | 2 |
| Keydate: | 2024-04-23 13:51:45+00 |
| Editdate: | 2025-01-27 16:35:22+00 |