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OAS accession Detail for 0291950
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| accessions_id: | 0291950 | archive |
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| Title: | Microsatellite genotypes of Breviolum sp. symbionts within Orbicella faveolata recruits outplanted to reefs in the Florida Keys from 2009-2011 (SymBioSys project) (NCEI Accession 0291950) |
| Abstract: | This dataset contains data collected from 2009-01-01 to 2011-12-31. These data include depth. These data were collected by David Sheets of Canisius College, Margaret W Miller of NOAA Southeast Fisheries Science Center, and Mary Alice Coffroth of University at Buffalo as part of the "Ontogenic change in Cnidarian-algal symbioses: A genomic and ecologic perspective (SymBioSys)" project. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-05-24. The following is the text of the dataset description provided by BCO-DMO: Acquisition Description: Sampling and Analytical Methodology: Egg-sperm bundles were collected from adult colonies of O. faveolata following the techniques described by Miller (2014). Larvae were reared at a shore-based lab and settled onto terracotta tiles . Once the larvae had metamorphosed and attached to the tiles, they were outplanted onto the reef by attachment vertically to a PVC rack approximately 0.2 m above the substrate to allow uptake of algal symbionts. Tiles with recruits were sampled one to three months after outplanting, recruits removed from the tile and preserved individually in 95% ethanol for subsequent molecular analysis. DNA extraction followed Coffroth et al. (1992). Genotypes of the algal symbionts within the genus Breviolum were characterized using three polymorphic microsatellite loci, B7Sym34, B7Sym36 and CA6.38, which were adapted for use with O. faveolata (Thornhill et al. 2009). Tissue samples were collected from the top, middle and bottom portions of adult Orbicella faveolata colonies using the syringe method as described in Correa et al. (2009) and DNA extracted following Coffroth et al. (1992). Cp-23S genotypes of the algal symbionts within the genus Breviolum were characterized following the protocol of Santos et al. (2003). Locations in the Florida Keys with Abbreviations Alligator (AR) Coral Garden (CG) Cheeca Rocks (CR) East Turtle (ET) Grecian Rocks (GR) Looe Key (LK) Sand Island (SI) Tennessee (TR) |
| Date received: | 20230524 |
| Start date: | 20090101 |
| End date: | 20111231 |
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| West boundary: | -81.4094 |
| East boundary: | -80.2833 |
| North boundary: | 25.1167 |
| South boundary: | 24.5449 |
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| Submitting institution: | Biological and Chemical Oceanography Data Management Office |
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| Metadata version: | 1 |
| Keydate: | 2024-04-27 17:37:31+00 |
| Editdate: | 2024-04-27 17:37:49+00 |