Proteomic Signatures of Corals from Thermodynamic Reefs (NCEI Accession 0216077)
In this work, I took a protein profiling approach known as iTRAQ approach to characterize and quantify the proteins synthesized by 12 experimental coral samples. Specifically, corals were collected from an upwelling site in the Luzon Strait ("Houbihu"; 21°56’18.01’’N, 120°44’45.54’’E) and a non-upwelling "control" site in the nearby Taiwan Strait ("Houwan"; 22°01'23.30"N, 120°41'18.29"E) and exposed to either highly variable (upwelling-simulating) or stable temperatures in the laboratory for seven days prior to sacrificing for protein analysis (the "Seriatopora hystrix variable temperature study" [SHVTS]). In this submission, I have uploaded the proofs of the manuscript describing the proteomic data (Mayfield in press Microorganisms), two RAW files (SHVTS-A & SHVTS-B for iTRAQ batches A & B, respectively), two MZML (open-access mass spectrometry data) files (SHVTS-A.mzml & SHVTS-B.mzml), two MZID files (SHVTS-A.mzid & SHVTS-B.mzid), and a tab-delimited file that describes which samples were labeled with which iTRAQ tag ("Mayfield online supplemental data file"). I have also uploaded the supplemental material that accompanies the Mayfield (in press) article since that is what readers interested in the specifics of the proteomic analysis pipeline should consult. The peak data were queried against a conceptually translated S. hystrix-Cladocopium dinoflagellate transcriptome described in Mayfield et al. (2016 Mol Ecol) and found here: http://symbiont.iis.sinica.edu.tw/s_hystrix/static/html/#stat. Please note that all proteomic data represent a mix of multiple organisms (namely the anthozoan host and the dinoflagellate endosymbionts that inhabit their gastrodermal cells). All data analysis was conducted with Proteome Discoverer (Thermo-Fisher Scientific).
Dataset Citation
- Cite as: Mayfield, Anderson B.; Cooperative Institute for Marine and Atmospheric Studies, University of Miami; Atlantic Oceanographic and Meteorological Laboratory (AOML) (2020). Proteomic Signatures of Corals from Thermodynamic Reefs (NCEI Accession 0216077). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0216077. Accessed [date].
Dataset Identifiers
ISO 19115-2 Metadata
gov.noaa.nodc:0216077
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Ordering Instructions | Contact NCEI for other distribution options and instructions. |
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NOAA National Centers for Environmental Information +1-301-713-3277 ncei.info@noaa.gov |
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NOAA National Centers for Environmental Information ncei.info@noaa.gov |
Time Period | 2010-05-14 to 2010-06-15 |
Spatial Bounding Box Coordinates |
West: 120.68841
East: 120.74598
South: 21.93834
North: 22.02314
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Dataset Progress Status | Complete - production of the data has been completed Historical archive - data has been stored in an offline storage facility |
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Purpose | These data were collected to better understand the cellular biology of thermo-tolerant corals (i.e., those that can tolerate wide fluctuations in temperature). For more details, please see the articles referenced in the data package and on my personal website: coralreefdiagnostics.com. |
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Last Modified: 2025-02-08T19:52:24Z
For questions about the information on this page, please email: ncei.info@noaa.gov
For questions about the information on this page, please email: ncei.info@noaa.gov